Model-based analysis of the role of biological, hydrological and geochemical factors affecting uranium bioremediation.


:Uranium contamination is a serious concern at several sites motivating the development of novel treatment strategies such as the Geobacter-mediated reductive immobilization of uranium. However, this bioremediation strategy has not yet been optimized for the sustained uranium removal. While several reactive-transport models have been developed to represent Geobacter-mediated bioremediation of uranium, these models often lack the detailed quantitative description of the microbial process (e.g., biomass build-up in both groundwater and sediments, electron transport system, etc.) and the interaction between biogeochemical and hydrological process. In this study, a novel multi-scale model was developed by integrating our recent model on electron capacitance of Geobacter (Zhao et al., 2010) with a comprehensive simulator of coupled fluid flow, hydrologic transport, heat transfer, and biogeochemical reactions. This mechanistic reactive-transport model accurately reproduces the experimental data for the bioremediation of uranium with acetate amendment. We subsequently performed global sensitivity analysis with the reactive-transport model in order to identify the main sources of prediction uncertainty caused by synergistic effects of biological, geochemical, and hydrological processes. The proposed approach successfully captured significant contributing factors across time and space, thereby improving the structure and parameterization of the comprehensive reactive-transport model. The global sensitivity analysis also provides a potentially useful tool to evaluate uranium bioremediation strategy. The simulations suggest that under difficult environments (e.g., highly contaminated with U(VI) at a high migration rate of solutes), the efficiency of uranium removal can be improved by adding Geobacter species to the contaminated site (bioaugmentation) in conjunction with the addition of electron donor (biostimulation). The simulations also highlight the interactive effect of initial cell concentration and flow rate on U(VI) reduction.


Biotechnol Bioeng


Zhao J,Scheibe TD,Mahadevan R




Has Abstract


2011-07-01 00:00:00












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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Aldarf M,Fourcade F,Amrane A,Prigent Y

    更新日期:2004-07-05 00:00:00

  • Simulations of host-plasmid interactions in Escherichia coli: Copy number, promoter strength, and ribosome binding site strength effects on metabolic activity and plasmid gene expression.

    abstract::A mechanistically detailed single-cell model E. coli B/r-A was adapted to simulate the effects of vector presence on cell metabolism. Competition for RNA polymerase between chromosome and plasmid DNA is explicitly included. Distribution of active ribosomes among chromosome- and plasmid-derived messenger RNA, another k...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Peretti SW,Bailey JE

    更新日期:1987-02-01 00:00:00

  • Estimation of the true growth yield and maintenance coefficient for yoghurt cultures.

    abstract::Point and 95% confidence interval estimates of the true growth yield and maintenance coefficient are reported for four pure cultures andtwo mixed cultures of Lactobacillus bulgaricus and Streptococcus thermophilus grown anaerobically on 3% nonfat dry milk using batch followed by fed batch culture. An improved method o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Oner MD,Erickson LE,Yang SS

    更新日期:1986-06-01 00:00:00

  • Production of recombinant proteins using multiple-copy gene integration in high-cell-density fermentations of Ralstonia eutropha.

    abstract::We have previously reported the development of a novel protein expression system based on Ralstonia eutropha. In this study we report on the influence of gene copynumber on recombinant protein expression in R. eutropha. We compare recombinant gene stability and expression levels of chromosomal integration with a plasm...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Srinivasan S,Barnard GC,Gerngross TU

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  • Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment.

    abstract::Targeted gene integration into site-specific loci can be achieved in Chinese hamster ovary (CHO) cells via CRISPR/Cas9 genome editing technology and the homology-directed repair (HDR) pathway. The low efficiency of HDR often requires antibiotic selection, which limits targeted integration of multiple genes at multiple...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lee JS,Grav LM,Pedersen LE,Lee GM,Kildegaard HF

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: de Marañón IM,Gervais P,Molin P

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    abstract::On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tsai PS,Rao G,Bailey JE

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    abstract::Although sophorolipids (SLs) produced by S. bombicola are a real showcase for the industrialization of microbial biosurfactants, some important drawbacks are associated with this efficient biological process, e.g., the simultaneous production of acidic and lactonic SLs. Depending on the application, there is a require...

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    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ladero M,Perez MT,Santos A,Garcia-Ochoa F

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  • Unified kinetic treatment for growth on dual nutrients.

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Haas CN

    更新日期:1994-06-20 00:00:00

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    authors: Barminko JA,Nativ NI,Schloss R,Yarmush ML

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    authors: Lavenson DM,Tozzi EJ,McCarthy MJ,Powell RL

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tholudur A,Ramirez WF,McMillan JD

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  • Physiologic mechanisms of sequential products synthesis in 1,3-propanediol fed-batch fermentation by Klebsiella pneumoniae.

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zheng ZM,Xu YZ,Liu HJ,Guo NN,Cai ZZ,Liu DH

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  • Bioprocess considerations for expanded-bed chromatography of crude canola extract: sample preparation and adsorbent reuse.

    abstract::Compared to the conventional microbial and mammalian systems, transgenic plants produce proteins in a different matrix. This provides opportunities and challenges for downstream processing. In the context of the plant host Brassica napus (canola), this work addresses the bioprocessing challenges of solid fractionation...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bai Y,Glatz CE

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    authors: Laluce C,Palmieri MC,Lopes da Cruz RC

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    authors: Liu N,Soong YV,Mirzaee I,Olsen A,Yu P,Wong HW,Xie D

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    authors: Larroche C,Besson I,Gros JB

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    pub_type: 杂志文章


    authors: Betenbaugh MJ,Dhurjati P

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Brühlmann F,Chen W

    更新日期:1999-06-05 00:00:00