Abstract:
:A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs. Leakage of protein from the CGAs and the activity of the adsorbed lipase decreased with increasing enzyme loading; this indicates that multilayers of lipase may be adsorbing onto the CGAs. The CGA-immobilised lipase displayed normal Michaelis-Menten dependence on substrate concentration and also exhibited greater activity than the free enzyme.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
O'Connell PJ,Varley Jdoi
10.1002/bit.1116subject
Has Abstractpub_date
2001-08-05 00:00:00pages
264-9issue
3eissn
0006-3592issn
1097-0290pii
10.1002/bit.1116journal_volume
74pub_type
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