Abstract:
:As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) in mesenchymal stem cells (MSC) derived from bone marrow of rats. For this purpose, rat MSCs transduced with TGM2_v2 were grown and differentiated on three-dimensional polybutylene succinate (PBSu) and poly-l-lactide (PLLA) blend scaffolds. The transduced cells could not only successfully express the short form transglutaminase-2, but also deposited the protein onto the scaffolds. In addition, they could spontaneously produce cartilage-specific proteins without any chondrogenic induction, suggesting that TGM2_v2 expression provided the cells the ability of chondrogenic differentiation. PBSu:PLLA scaffolds loaded with TGM2_v2 expressing MSCs could be used in repair of articular cartilage defects.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Calikoglu Koyuncu AC,Nayman AH,Telci D,Torun Kose Gdoi
10.1002/bit.27311subject
Has Abstractpub_date
2020-06-01 00:00:00pages
1839-1852issue
6eissn
0006-3592issn
1097-0290journal_volume
117pub_type
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