Abstract:
:The design of new generation bioprocessing plants is increasingly dependent on the design of process-compatible microorganisms. The latter, whether through genetic or physiological manipulations, can be greatly assisted by metabolic engineering. An emerging powerful tool in metabolic engineering research is computer-assisted cell design using mathematical programming. In this work, the problem of optimizing cellular metabolic networks has been formulated as a Mixed Integer Nonlinear Programming (MINLP) model. The model can assist genetic engineers to identify which cellular enzymes should be modified, and the new levels of activity required to produce an optimal network. Results are presented from the tricarboxylic acid cycle in Dictyostelium discoideum. Copyright 1998 John Wiley & Sons, Inc.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Dean JP,Dervakos GAdoi
10.1002/(sici)1097-0290(19980420)58:2/3<267::aid-bsubject
Has Abstractpub_date
1998-04-05 00:00:00pages
267-71issue
2-3eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(19980420)58:2/3<267::AID-Bjournal_volume
58pub_type
杂志文章abstract::Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370207
更新日期:1991-01-20 00:00:00
abstract::The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::A refolding strategy was described for on-column refolding of recombinant human interferon-gamma (rhIFN-gamma) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-gamma protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded bed ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20763
更新日期:2006-03-05 00:00:00
abstract::The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single myceliu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260470513
更新日期:1995-09-05 00:00:00
abstract::A packed granular activated carbon (GAC) biobed, inoculated with the ethane-degrading strain Mycobacterium E3, was used to study ethene removal from a synthetic waste gas. Ethene, for which the dimensionless partition coefficient for an air-water system at 20 degrees C is about 7.6, was used as a model compound for po...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260440511
更新日期:1994-08-20 00:00:00
abstract::Propionic acid (PA) and its salts are widely used in the food, pharmaceutical, and chemical industries. Microbial production of PA by propionibacteria is a typical product-inhibited process, and acid resistance is crucial in the improvement of PA titers and productivity. We previously identified two key acid resistanc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25902
更新日期:2016-06-01 00:00:00
abstract::The G-H loop of foot-and-mouth disease virus is a disordered protrusion of the VP1 protein exposed on the virion surface. This short stretch includes an arginine-glycine-aspartic acid tripeptide, a recognized integrin-binding motif, which is responsible for cell attachment and infection. Eight copies of a peptide repr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980805)59:3<294::aid-bit
更新日期:1998-08-05 00:00:00
abstract::To initiate an infection human immunodeficiency virus type 1 (HIV-1) particles must first bind to receptors on the surface of their host cells, a process that eventually leads to fusion of viral and cellular membranes and release of the viral genome into the cytoplasm. Understanding the molecular mechanisms of these p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20680
更新日期:2006-02-05 00:00:00
abstract::Numerous studies on poly γ-d-glutamicacid (γ-PGA) production have investigated terrestrial renewable sources for reducing production costs, but there are no studies using waste marine resources so far. We aimed to develop a cost-effective production method of γ-d-PGA by Bacillus sp. SJ-10 using green macroalgae (Ulva ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26966
更新日期:2019-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10487
更新日期:2003-03-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24351
更新日期:2012-03-01 00:00:00
abstract::Intracellular metabolite concentration and enzyme activity measurements were made to explain the new metabolic and growth phenomena seen in the micro-aerobic, continuous yeast cultures described in Part I. The results of these assays suggested mechanisms for the observed maximum in the specific ethanol productivity as...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260361007
更新日期:1990-12-05 00:00:00
abstract::Surface modified bioactive glass with surface properties akin to those of the bone mineral phase is an attractive candidate for use as a microcarrier material for 3-D growth of bone-like tissue in rotating wall vessel bioreactors (RWVs). The critical surface properties of this material are the result of reaction in so...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10023
更新日期:2001-11-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27656
更新日期:2020-12-15 00:00:00
abstract::Biomass autoflocculation in outdoor algal cultures was found to be associated with increases of culture pH levels, due to CO(2) consumption by the algal photosynthetic activity. Under these alkaline conditions, some medium chemical ions precipitated together with the algal biomass. The chemical substances involved wit...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260206
更新日期:1984-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10788
更新日期:2004-01-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260191007
更新日期:1977-10-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270407
更新日期:1985-04-01 00:00:00
abstract::Autotrophic or mixotrophic use of one-carbon (C1) compounds is gaining importance for sustainable bioproduction. In an effort to integrate the reductive glycine pathway (rGP) as a highly promising pathway for the assimilation of CO2 and formate, genes coding for glycine synthase system from Gottschalkia acidurici were...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27658
更新日期:2020-12-17 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20546
更新日期:2005-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21386
更新日期:2007-06-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260401008
更新日期:1992-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24874
更新日期:2013-08-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370914
更新日期:1991-04-15 00:00:00
abstract::Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990805)64:3<333::aid-bit
更新日期:1999-08-05 00:00:00
abstract::Continuous propionic acid fermentations of lactate by Propionibacterium acidipropionici were studied in spiral wound fibrous bed bioreactors. Cells were imobilized by natural attachment to fiber surfaces and entrapment in the void volume within the fibrous matrix. A high cell density of approximately 37 g/L was attain...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400404
更新日期:1992-08-05 00:00:00
abstract::The development of renewable alternatives to diesel and jet fuels is highly desirable for the heavy transportation sector, and would offer benefits over the production and use of short-chain alcohols for personal transportation. Here, we report the development of a metabolically engineered strain of Escherichia coli t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22660
更新日期:2010-06-01 00:00:00
abstract::Natural oxygen gradients occur in tissues of biological organisms and also in the context of three-dimensional (3D) in vitro cultivation. Oxygen diffusion limitation and metabolic oxygen consumption by embedded cells produce areas of hypoxia in the tissue/matrix. However, reliable systems to detect oxygen gradients an...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27503
更新日期:2020-07-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260371009
更新日期:1991-04-25 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22254
更新日期:2009-04-01 00:00:00