Novel non-viral vectors for gene delivery: synthesis of a second-generation library of mono-functionalized poly-(guanidinium)amines and their introduction into cationic lipids.

Abstract:

:The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthesized a secondary library of mono-functionalized poly-(guanidinium)amines generated from a library of mono-functionalized polyamines applying the concept of "libraries from libraries." The method allows a quick and easy access to mono-functionalized geometrically varied poly-(guanidinium)amines. The new building blocks were introduced into cationic lipids to obtain novel poly-(guanidinium)amine lipids, which are potential DNA vectors for gene delivery.

journal_name

Biotechnol Bioeng

authors

Byk G,Soto J,Mattler C,Frederic M,Scherman D

subject

Has Abstract

pub_date

1998-04-01 00:00:00

pages

81-7

issue

2

eissn

0006-3592

issn

1097-0290

pii

10.1002/(SICI)1097-0290(199821)61:2<81::AID-BIT1>3

journal_volume

61

pub_type

杂志文章
  • Acetoclastic methanogenic activity measurement by a titration bioassay.

    abstract::A titration bioassay, designed to accurately determine the activity of acetoclastic methanogens, is described that also allows evaluation of inhibition due to potential toxicants on the active biomass. The instrument is made of a pH-stat connected to an anaerobic batch reactor. Acetate is blended and mixed with anaero...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10088

    authors: Rozzi A,Castellazzi L,Speece RE

    更新日期:2002-01-05 00:00:00

  • Kinetics and response of a Pseudomonas fuorescens HK44 biosensor.

    abstract::The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. Th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19970605)54:5<491::AID-BIT

    authors: Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GS

    更新日期:1997-06-05 00:00:00

  • Chemical inhibition of nitrification in activated sludge.

    abstract::Conventional aerobic nitrification was adversely affected by single pulse inputs of six different classes of industrially relevant chemical toxins: an electrophilic solvent (1-chloro-2,4-dinitrobenzene, CDNB), a heavy metal (cadmium), a hydrophobic chemical (1-octanol), an uncoupling agent (2,4-dinitrophenol, DNP), al...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20015

    authors: Kelly RT 2nd,Henriques ID,Love NG

    更新日期:2004-03-20 00:00:00

  • Surface display of MPH on Pseudomonas putida JS444 using ice nucleation protein and its application in detoxification of organophosphates.

    abstract::Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21535

    authors: Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

    更新日期:2008-01-01 00:00:00

  • Microbial reduction of technetium by Escherichia coli and Desulfovibrio desulfuricans: enhancement via the use of high-activity strains and effect of process parameters.

    abstract::Escherichia coli and Desulfovibrio desulfuricans reduce Tc(VII) (TcO(4)(-)) with formate or hydrogen as electron donors. The reaction is catalyzed by the hydrogenase component of the formate hydrogenlyase complex (FHL) of E. coli and is associated with a periplasmic hydrogenase activity in D. desulfuricans. Tc(VII) re...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Lloyd JR,Thomas GH,Finlay JA,Cole JA,Macaskie LE

    更新日期:1999-01-01 00:00:00

  • Cascading effect in bioprocessing-The impact of mild hypothermia on CHO cell behavior and host cell protein composition.

    abstract::A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.26437

    authors: Goey CH,Tsang JMH,Bell D,Kontoravdi C

    更新日期:2017-12-01 00:00:00

  • Comparison of glucose fermentation by suspended and gel-entrapped yeast cells: An in vivo nuclear magnetic resonance study.

    abstract::Phosphorus-31 nuclear magnetic resonance ((31)P NMR) was used to compare the anaerobic metabolism of glucose by suspended and gel-entrapped Saccharomyces bayanus cells. The fermentation of glucose was carried out in a reaction system with continuous circulation through the NMR sample tube. The intracellular pH and the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260410607

    authors: Taipa MA,Cabral JM,Santos H

    更新日期:1993-03-15 00:00:00

  • Comparison of microbial community composition and activity in sulfate-reducing batch systems remediating mine drainage.

    abstract::Five microbial inocula were evaluated in batch tests for the ability to remediate mine drainage (MD). Dairy manure (DM), anaerobic digester sludge, substrate from the Luttrell (LUTR) and Peerless Jenny King (PJK) sulfate-reducing permeable reactive zones (SR-PRZs) and material from an MD-treatment column that had been...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21930

    authors: Pereyra LP,Hiibel SR,Pruden A,Reardon KF

    更新日期:2008-11-01 00:00:00

  • Hollow fiber bioartificial liver utilizing collagen-entrapped porcine hepatocyte spheroids.

    abstract::A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in pr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19961005)52:1<34::AID-BIT4

    authors: Wu FJ,Friend JR,Lazar A,Mann HJ,Remmel RP,Cerra FB,Hu WS

    更新日期:1996-10-05 00:00:00

  • The relationship between hydrogen gas and butanol production by Clostridium saccharoperbutylacetonicum.

    abstract::Two simultaneous fermentations were performed at 26 degrees C with simultaneous inocula using Clostridium saccharoperbutylacetonicum. Fermentation 1 prevented the gas formed by the biomass from escaping the fermentor while 2 allowed the gas formed to escape. Fermentor 1 provided for the production of butanol, acetone,...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260280302

    authors: Brosseau JD,Yan JY,Lo KV

    更新日期:1986-03-01 00:00:00

  • Functional phenotyping of human plasma using a 361-fluorogenic substrate biosensing microarray.

    abstract::A microarray presenting glycerol nanodroplets of fluorogenic peptide substrates was used as a biosensor for the detection of multiple enzyme activities within human plasma. Using 10 different plasma proteases (kallikrein, factor XIIa, factor XIa, factor IXa, factor VIIa, factor Xa, thrombin, activated protein C, uPA a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20927

    authors: Gosalia DN,Denney WS,Salisbury CM,Ellman JA,Diamond SL

    更新日期:2006-08-20 00:00:00

  • Cellulase immobilized on poly-L-glutamic acid.

    abstract::Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260290203

    authors: Takeuchi T,Makino K

    更新日期:1987-02-01 00:00:00

  • Partitioning of low molecular combination peptides in aqueous two-phase systems of poly(ethylene glycol) and dextran in the presence of small amounts of K2HPO4/KH2PO4 buffer at 293 K: experimental results and predictions.

    abstract::Buffered aqueous two-phase systems are effective extraction systems for separating amphoteric hydrocarbons like, for example, polypeptides from aqueous phases. The design and basic engineering of such processes requires the knowledge of the liquid-liquid equilibrium. The study presented here aims to contribute to the ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Grossmann C,Tintinger R,Zhu J,Maurer G

    更新日期:1998-12-20 00:00:00

  • Hydrodynamic modulation of embryonic stem cell differentiation by rotary orbital suspension culture.

    abstract::Embryonic stem cells (ESCs) can differentiate into all somatic cell types, but the development of effective strategies to direct ESC fate is dependent upon defining environmental parameters capable of influencing cell phenotype. ESCs are commonly differentiated via cell aggregates referred to as embryoid bodies (EBs),...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.22578

    authors: Sargent CY,Berguig GY,Kinney MA,Hiatt LA,Carpenedo RL,Berson RE,McDevitt TC

    更新日期:2010-02-15 00:00:00

  • Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae.

    abstract::Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.27226

    authors: Kiefer R,Jurisic M,Dahlem C,Koch M,Schmitt MJ,Kiemer AK,Schneider M,Breinig F

    更新日期:2020-03-01 00:00:00

  • Production of lovastatin examined by an integrated approach based on chemometrics and DOSY-NMR.

    abstract::Microbial secondary metabolites are one of the sources of therapeutic molecules in the pharmaceutical industry. Product quality and high yields of secondary metabolites are the main goals for the commercial success of a fermentation process. Our novel approach was based on the decision-tree algorithm to determine the ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10418

    authors: Bradamante S,Barenghi L,Beretta G,Bonfa' M,Rollini M,Manzoni M

    更新日期:2002-12-05 00:00:00

  • Dual substrate biodegradation of a nonionic surfactant and pentachlorophenol by Sphingomonas chlorophenolica RA2.

    abstract::The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biode...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20365

    authors: Bielefeldt AR,Cort T

    更新日期:2005-03-20 00:00:00

  • Improved virus purification processes for vaccines and gene therapy.

    abstract::The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral dow...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审

    doi:10.1002/bit.25545

    authors: Nestola P,Peixoto C,Silva RR,Alves PM,Mota JP,Carrondo MJ

    更新日期:2015-05-01 00:00:00

  • Development of a novel ER stress based selection system for the isolation of highly productive clones.

    abstract::Most biotherapeutic drugs are recombinant monoclonal antibodies which are mostly produced in monoclonal cell lines derived from Chinese hamster ovary (CHO) cells. Various clones expressing a monoclonal recombinant antibody were analyzed and a correlation of the antibody concentration and the relative mRNA level of cal...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.24527

    authors: Kober L,Zehe C,Bode J

    更新日期:2012-10-01 00:00:00

  • Surface transformation of bioactive glass in bioreactors simulating microgravity conditions. Part I: experimental study.

    abstract::Surface modified bioactive glass with surface properties akin to those of the bone mineral phase is an attractive candidate for use as a microcarrier material for 3-D growth of bone-like tissue in rotating wall vessel bioreactors (RWVs). The critical surface properties of this material are the result of reaction in so...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10023

    authors: Radin S,Ducheyne P,Ayyaswamy PS,Gao H

    更新日期:2001-11-05 00:00:00

  • A Kalman filter algorithm and monitoring apparatus for at-line control of fractional protein precipitation.

    abstract::Downstream processing operations are often carried out blind in the process timescale since product monitoring on-line is not common. Knowledge of the location and concentration of the product and key contaminants is complementary to other process information for process development and, if available on-line in conjun...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19970105)53:1<58::AID-BIT9

    authors: Holwill IJ,Chard SJ,Flanagan MT,Hoare M

    更新日期:1997-01-05 00:00:00

  • Measuring enzyme motility in organic media using novel H-D exchange methodology.

    abstract::A novel deuterium ((2)H) NMR technique as developed for measuring the total number of deuterons exchanged by lyophilised protein samples following hydrogen-deuterium (H-D) exchange. Using this methodology differences in the H-D exchange behaviour of the proteolytic enzyme subtilisin Carlsberg hydrated either in air or...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Hutcheon GA,Parker MC,Moore BD

    更新日期:2000-11-05 00:00:00

  • Yield and maintenance relations of yeast growth in the chemostat at superoptimal temperatures.

    abstract::A model is proposed that accounts for the decreases in yield which occur in chemostat cultures of mesophilic yeasts at superoptimal growth temperatures. Two yield depressing effects were identified, one due to increased maintenance requirements by the viable fraction of the population, the other due to energy substrat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260180603

    authors: Van Uden N,Madeira-Lopes A

    更新日期:1976-06-01 00:00:00

  • Production of savinase and population viability of Bacillus clausii during high-cell-density fed-batch cultivations.

    abstract::The growth and product formation of a Savinase-producing Bacillus clausii were investigated in high-cell-density fed-batch cultivations with both linear and exponential feed profiles. The highest specific productivity of Savinase was observed shortly after the end of the initial batch phase for all feed profiles appli...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10675

    authors: Christiansen T,Michaelsen S,Wümpelmann M,Nielsen J

    更新日期:2003-08-05 00:00:00

  • Introduction of glycine synthase enables uptake of exogenous formate and strongly impacts the metabolism in Clostridium pasteurianum.

    abstract::Autotrophic or mixotrophic use of one-carbon (C1) compounds is gaining importance for sustainable bioproduction. In an effort to integrate the reductive glycine pathway (rGP) as a highly promising pathway for the assimilation of CO2 and formate, genes coding for glycine synthase system from Gottschalkia acidurici were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.27658

    authors: Hong Y,Arbter P,Wang W,Rojas LN,Zeng AP

    更新日期:2020-12-17 00:00:00

  • C-terminal lysine variants in fully human monoclonal antibodies: investigation of test methods and possible causes.

    abstract::The C-terminal lysine variation is commonly observed in biopharmaceutical monoclonal antibodies. This modification can be important since it is found to be sensitive to the production process. The methods commonly used to probe this charge variation, including IEF, cIEF, ion-exchange chromatography, and LC-MS, were ev...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21855

    authors: Dick LW Jr,Qiu D,Mahon D,Adamo M,Cheng KC

    更新日期:2008-08-15 00:00:00

  • Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition.

    abstract::Retroviral-derived biopharmaceuticals (RV) target numerous therapeutic applications, from gene therapy to virus-like particle (rVLP)-based vaccines. During particle formation, beside the pseudotyped envelope proteins, RV can incorporate proteins derived from the virus producer cells (VPC). This may be detrimental by r...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.23231

    authors: Rodrigues AF,Guerreiro MR,Santiago VM,Dalba C,Klatzmann D,Alves PM,Carrondo MJ,Coroadinha AS

    更新日期:2011-11-01 00:00:00

  • An algorithm for operating a fed-batch fermentor at optimum specific-growth rate.

    abstract::An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260330115

    authors: Agrawal P,Koshy G,Ramseier M

    更新日期:1989-01-05 00:00:00

  • Improvement of the production of GFPuv-beta1,3-N-acetylglucosaminyltransferase 2 fusion protein using a molecular chaperone-assisted insect-cell-based expression system.

    abstract::A stable Tn-5B1-4 insect cell line co-expressing the recombinant GFPuv-beta1,3-N-acetylglucosaminyltransferase 2 (GFPuv-beta3GnT2) protein fused to a melittin signal sequence with a lectin-like molecular chaperone, human calnexin (hCNX) or human calreticulin (hCRT), was constructed. The expression of either of these m...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20362

    authors: Kato T,Murata T,Usui T,Park EY

    更新日期:2005-02-20 00:00:00

  • Modeling thrombus formation and growth.

    abstract::The paper reviews the state-of-the-art in computational modeling of thrombus formation and growth and related phenomena including platelet margination, activation, adhesion, and embolization. Presently, there is a high degree of empiricism in the modeling of thrombus formation. Based on the experimentally observed phy...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审

    doi:10.1002/bit.26343

    authors: Hosseinzadegan H,Tafti DK

    更新日期:2017-10-01 00:00:00