Abstract:
:A refolding strategy was described for on-column refolding of recombinant human interferon-gamma (rhIFN-gamma) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-gamma protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded bed by gradually decreasing the concentration of urea in the buffer and the refolded rhIFN-gamma protein was recovered by the elution in packed bed mode. It was demonstrated that the denatured rhIFN-gamma protein could be efficiently refolded by this method with high yield. Under appropriate experimental conditions, the protein yield and specific activity of rhIFN-gamma was up to 52.7% and 8.18 x 10(6) IU/mg, respectively.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Jin T,Guan YX,Yao SJ,Lin DQ,Cho MGdoi
10.1002/bit.20763subject
Has Abstractpub_date
2006-03-05 00:00:00pages
755-60issue
4eissn
0006-3592issn
1097-0290journal_volume
93pub_type
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