Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.

Abstract:

:Metabolically active resting (i.e., nongrowing) bacterial cells have a high potential in cofactor-dependent redox biotransformations. Where growing cells require carbon and energy for biomass production, resting cells can potentially exploit their metabolism more efficiently for redox biocatalysis allowing higher specific activities and product yields on energy source. Here, the potential of resting recombinant E. coli containing the styrene monooxygenase StyAB was investigated for enantioselective styrene epoxidation in a two-liquid phase setup. Resting cells indeed showed twofold higher specific activities as compared to growing cells in a similar setup. However, product formation rates decreased steadily resulting in lower final product concentrations. The low intrinsic stability of the reductase component StyB was found to limit overall biocatalyst stability. Such limitation by enzyme stability was overcome by increasing intracellular StyB levels. Beyond that, product inhibition was identified as a limiting factor, whereas complete toxification of the bacterial cells, as it was observed with growing cells, and deactivation of the multicomponent enzyme system did not occur. The resting cell setup allowed high product yields on glucose of more than 5 mol mol(glucose)(-1), which makes the use of resting cells a promising approach for ecologically as well as economically sustainable oxygenase-based whole-cell biocatalysis.

journal_name

Biotechnol Bioeng

authors

Julsing MK,Kuhn D,Schmid A,Bühler B

doi

10.1002/bit.24404

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

1109-19

issue

5

eissn

0006-3592

issn

1097-0290

journal_volume

109

pub_type

杂志文章
  • Application of high throughput pretreatment and co-hydrolysis system to thermochemical pretreatment. Part 2: Dilute alkali.

    abstract::High throughput pretreatment (HTPH) and enzymatic hydrolysis systems are now vital for screening large numbers of biomass samples to investigate biomass recalcitrance over various pretreatment and enzymatic hydrolysis conditions. Although hydrothermal pretreatment is currently being employed in most high throughput ap...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.24951

    authors: Li H,Gao X,Demartini JD,Kumar R,Wyman CE

    更新日期:2013-11-01 00:00:00

  • Pulsed addition of limiting-carbon during Aspergillus oryzae fermentation leads to improved productivity of a recombinant enzyme.

    abstract::Fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. The objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10548

    authors: Bhargava S,Wenger KS,Marten MR

    更新日期:2003-04-05 00:00:00

  • Metabolic engineering of Ralstonia eutropha for the production of polyhydroxyalkanoates from sucrose.

    abstract::A sucrose utilization pathway was established in Ralstonia eutropha NCIMB11599 and R. eutropha 437-540 by introducing the Mannheimia succiniciproducens MBEL55E sacC gene that encodes β-fructofuranosidase. These engineered strains were examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.25469

    authors: Park SJ,Jang YA,Noh W,Oh YH,Lee H,David Y,Baylon MG,Shin J,Yang JE,Choi SY,Lee SH,Lee SY

    更新日期:2015-03-01 00:00:00

  • Covalent immobilization of redox enzyme on electrospun nonwoven poly(acrylonitrile-co-acrylic acid) nanofiber mesh filled with carbon nanotubes: a comprehensive study.

    abstract::In this work, novel conductive composite nanofiber mesh possessing reactive groups was electrospun from solutions containing poly(acrylonitrile-co-acrylic acid) (PANCAA) and multi-walled carbon nanotubes (MWCNTs) for redoxase immobilization, assuming that the incorporated MWCNTs could behave as electrons transferor du...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21280

    authors: Wang ZG,Ke BB,Xu ZK

    更新日期:2007-07-01 00:00:00

  • Engineering the pentose phosphate pathway to improve hydrogen yield in recombinant Escherichia coli.

    abstract::Among various routes for the biological hydrogen production, the NAD(P)H-dependent pentose phosphate (PP) pathway is the most efficient for the dark fermentation. Few studies, however, have focused on the glucose-6-phosphate 1-dehydrogenase, encoded by zwf, as a key enzyme activating the PP pathway. Although the gluco...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.23259

    authors: Kim YM,Cho HS,Jung GY,Park JM

    更新日期:2011-12-01 00:00:00

  • Importance of spore mutants for fed-batch and continuous fermentation of Bacillus subtilis.

    abstract::To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260470610

    authors: Oh MK,Kim BG,Park SH

    更新日期:1995-09-20 00:00:00

  • Stimulating in-soil rhamnolipid production in a bioslurry reactor by limiting nitrogen.

    abstract::A soil with aged contamination from lubricating oil (LO) and polychlorinated biphenyls (PCBs) was treated in a bioslurry reactor to investigate in-soil biosurfactant production by Pseudomonas aeruginosa, the most abundant indigenous, culturable, hydrocarbon-degrading microorganism. After 2 days of growth on LO, a depl...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20295

    authors: Hudak AJ,Cassidy DP

    更新日期:2004-12-30 00:00:00

  • Biofilm thickness variability investigated with a laser triangulation sensor.

    abstract::Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the mo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/1097-0290(20001220)70:6<619::aid-bit3>3.0.

    authors: Okkerse WJ,Ottengraf SP,Osinga-Kuipers B

    更新日期:2000-12-20 00:00:00

  • Biological destruction of CCl(4): I. Experimental design and data.

    abstract::A denitrifying consortium capable of transforming carbon tetrachloride (CCl(4)) was cultured from aquifer sediment from the U.S. Department of Energy's Hanford Site in southeastern Washington State. To understand the kinetics of the biological destruction of CCl(4) by these microbes, a set of experiments, the conditio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260430613

    authors: Petersen JN,Skeen RS,Amos KM,Hooker BS

    更新日期:1994-03-15 00:00:00

  • Engineering of molecular and cellular biocatalysts: selected contributions by James E. Bailey.

    abstract::James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...

    journal_title:Biotechnology and bioengineering

    pub_type: 传,历史文章,杂志文章

    doi:10.1002/bit.10404

    authors: Dordick JS,Khosla C

    更新日期:2002-09-05 00:00:00

  • Effects of humic substances and soya lecithin on the aerobic bioremediation of a soil historically contaminated by polycyclic aromatic hydrocarbons (PAHs).

    abstract::The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) strongly reduces their bioavailability in aged contaminated soils, thus limiting their bioremediation. The biodegradation of PAHs in soils can be enhanced by employing surface-active agents. However, chemical surfactants are often recalcitrant and exer...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20225

    authors: Fava F,Berselli S,Conte P,Piccolo A,Marchetti L

    更新日期:2004-10-20 00:00:00

  • Use of the abiotic proton balance for determining linear relations among net conversion rates of primary metabolites in fermentation processes.

    abstract::Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19961220)52:6<713::AID-BIT

    authors: Siano SA

    更新日期:1996-12-20 00:00:00

  • Role of some whey components on mass transfer in ultrafiltration.

    abstract::Ultrafiltration through Carbosep M(4) mineral membrane of protein solutions of decreasing complexity (whey before and after centrifugation or clarification, beta-lactoglobulin) was studied. Mathematical models were used to explain variations in flux with time. Taking into account variations in protein retention and hy...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260340205

    authors: Taddei C,Daufin G,Aimar P,Sanchez V

    更新日期:1989-06-20 00:00:00

  • Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture.

    abstract::Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10397

    authors: Kim Y,Arp DJ,Semprini L

    更新日期:2002-12-05 00:00:00

  • A new nodavirus-negative Trichoplusia ni cell line for baculovirus-mediated protein production.

    abstract::Cell lines derived from Trichoplusia ni (Tn) are widely used as hosts in the baculovirus-insect cell system (BICS). One advantage of Tn cell lines is they can produce recombinant proteins at higher levels than cell lines derived from other insects. However, Tn cell lines are persistently infected with an alphanodaviru...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.27494

    authors: Maghodia AB,Geisler C,Jarvis DL

    更新日期:2020-07-14 00:00:00

  • Optimizing normoxic conditions in liver devices using enhanced gel matrices.

    abstract::For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of the liver, they must at least successfully support hepatocytes, the parenchymal cell of the liver. In vivo, the liver is a major consumer of oxygen. Hence it is unsurprising that the limited transport distanc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21681

    authors: Niu M,Clemens MG,Coger RN

    更新日期:2008-04-15 00:00:00

  • Conversion and toxicity characteristics of formaldehyde in acetoclastic methanogenic sludge.

    abstract::An unadapted mixed methanogenic sludge transformed formaldehyde into methanol and formate. The methanol to formate ratio obtained was 1:1. Formaldehyde conversion proceeded without any lag phase, suggesting the constitutive character of the formaldehyde conversion enzymes involved. Because the rate of formaldehyde con...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10294

    authors: Gonzalez-Gil G,Kleerebezem R,Lettinga G

    更新日期:2002-08-05 00:00:00

  • Transcriptome dynamics of transgene amplification in Chinese hamster ovary cells.

    abstract::Dihydrofolate reductase (DHFR) system is used to amplify the product gene to multiple copies in Chinese Hamster Ovary (CHO) cells for generating cell lines which produce the recombinant protein at high levels. The physiological changes accompanying the transformation of the non-protein secreting host cells to a high p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.25117

    authors: Vishwanathan N,Le H,Jacob NM,Tsao YS,Ng SW,Loo B,Liu Z,Kantardjieff A,Hu WS

    更新日期:2014-03-01 00:00:00

  • Hollow fiber supported gas membrane for in situ removal of ammonium during an antibiotic fermentation.

    abstract::To study the influence of ammonium on an antibiotic cultivation, mass transfer measurements of ammonium through microporous hydrophobic membranes using different stripping methods were carried out and compared. The higher overall mass transfer coefficients for ammonium were obtained with an acid stripping solution com...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260351012

    authors: Hecht V,Bischoff L,Gerth K

    更新日期:1990-04-25 00:00:00

  • Live cell imaging compatible immobilization of Chlamydomonas reinhardtii in microfluidic platform for biodiesel research.

    abstract::This paper describes a novel surface immobilization method for live-cell imaging of Chlamydomonas reinhardtii for continuous monitoring of lipid droplet accumulation. Microfluidics allows high-throughput manipulation and analysis of single cells in precisely controlled microenvironment. Fluorescence imaging based quan...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.25453

    authors: Park JW,Na SC,Nguyen TQ,Paik SM,Kang M,Hong D,Choi IS,Lee JH,Jeon NL

    更新日期:2015-03-01 00:00:00

  • Countercurrent gradient chromatography: A continuous focusing technique.

    abstract::The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and rem...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260480508

    authors: Evans LL,Burns MA

    更新日期:1995-12-05 00:00:00

  • Models for high cell density bioreactors must consider biomass volume fraction: Cell recycle example.

    abstract::Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260290906

    authors: Monbouquette HG

    更新日期:1987-06-01 00:00:00

  • Influence of reaction and diffusion on spatial organization of mitochondria and effectiveness factors in skeletal muscle cell design.

    abstract::A mathematical model is developed to analyze the influence of chemical reaction and diffusion processes on the intracellular organization of mitochondria in skeletal muscle cells. The mathematical modeling approach uses a reaction-diffusion analysis of oxygen, ATP, and ADP involved in energy metabolism and mitochondri...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.23112

    authors: Pathi B,Kinsey ST,Locke BR

    更新日期:2011-08-01 00:00:00

  • In vitro and in vivo enzyme studies of polyhemoglobin-tyrosinase.

    abstract::Melanoma is now the fifth most common type of cancer in North America. At present, there is no optimal treatment for this cancer. However, the lowering of the tyrosine level can inhibit the growth of melanoma. Unfortunately, this diet restriction cannot be humanly tolerated and causes vomiting, nausea, and severe body...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20084

    authors: Yu B,Chang TM

    更新日期:2004-06-30 00:00:00

  • Cell growth and differentiation on feeder layers is predicted to be influenced by bioreactor geometry.

    abstract::Tissue function is comprised of a complex interplay between biological and physicochemical rate processes. The design of bioreactors for tissue engineering must account for these processes simultaneously in order to obtain a bioreactor that provides a uniform environment for tissue growth and development. In the prese...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19960605)50:5<479::AID-BIT

    authors: Peng CA,Palsson BØ

    更新日期:1996-06-05 00:00:00

  • Plasmid stability in budding yeast populations: Steady-state growth with selection pressure.

    abstract::Plasmid gene product accumulation in a cell population depends on the fraction of plasmid-containing cells and the distribution of single-cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single-cell leve...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260260519

    authors: Hjortso MA,Bailey JE

    更新日期:1984-05-01 00:00:00

  • Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells.

    abstract::In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260400125

    authors: van der Pol L,Bakker WA,Tramper J

    更新日期:1992-06-05 00:00:00

  • Quantification of cell culture factors affecting recombinant protein yields in baculovirus-infected insect cells.

    abstract::An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recomb...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260390605

    authors: Lindsay DA,Betenbaugh MJ

    更新日期:1992-03-15 00:00:00

  • Image analysis to quantify and measure UASB digester granules.

    abstract::Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260420303

    authors: Dudley BT,Howgrave-Graham AR,Bruton AG,Wallis FM

    更新日期:1993-07-01 00:00:00

  • Gut-liver on a chip toward an in vitro model of hepatic steatosis.

    abstract::Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. This model does not reca...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.26793

    authors: Lee SY,Sung JH

    更新日期:2018-11-01 00:00:00