Abstract:
:A process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow-fiber ultrafiltration technology under conditions of large-scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5-fold during concentration. Thermal inactivation of BaEV was retarded by the addition of lactose glutamate to the harvested tissue culture fluid. After concentration, at least 91% of the virus RNA-directed DNA polymerase is recovered with a concomitant increase in infectious virus. Materials needed for modifying described systems may be obtained from commercial sources.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Weiss SAdoi
10.1002/bit.260220103subject
Has Abstractpub_date
1980-01-01 00:00:00pages
19-31issue
1eissn
0006-3592issn
1097-0290journal_volume
22pub_type
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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