Abstract:
:Catalytic membranes, obtained by immobilizing thermophilic beta-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature gradients affect enzyme activity. The results showed that the thermophilic enzyme behaved as the mesophilic beta-galactosidase, exhibiting an activity increase which was linearly proportional to the transmembrane temperature difference. The efficiency of the system proposed was determined by calculating two constants, alpha and beta, which represent respectively the percentage increase of enzyme activity when a temperature difference of 1 degrees C or a temperature gradient of 1 degrees C cm-1 were applied across the catalytic membrane. The increase of enzyme activity in nonisothermal bioreactors entailed a proportional reduction of production times. The advantages in using thermophilic enzymes immobilized in nonisothermal bioreactors are also discussed.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Febbraio F,Portaccio M,Stellato S,Rossi S,Bencivenga U,Nucci R,Rossi M,Gaeta FS,Mita DGsubject
Has Abstractpub_date
1998-07-05 00:00:00pages
108-15issue
1eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(19980705)59:1<108::AID-BITjournal_volume
59pub_type
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