Abstract:
:Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlactone functional groups. Optimization of hydrophilic/hydrophobic properties of the monolith affords a support that does not shrink in water and leads to immobilized enzyme that shows high activity in the hydrolysis of both low and high molecular weight substrates such as L-benzoyl arginine ethyl ester and casein. The catalytic activity of the monolithic reactor is maintained even at a flow velocity of 180 cm/min, which substantially exceeds those reported in the literature for packed bed reactors.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Xie S,Svec F,Fréchet JMdoi
10.1002/(sici)1097-0290(19990105)62:1<30::aid-bit4subject
Has Abstractpub_date
1999-01-05 00:00:00pages
30-5issue
1eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(19990105)62:1<30::AID-BIT4journal_volume
62pub_type
杂志文章abstract::The purpose of this study was to understand bacteria profile modification and its applications in subsurface biological operations such as biobarrier formation, in situ bioremediation, and microbial-enhanced oil recovery. Biomass accumulation and evolution in porous media were investigated both experimentally and theo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000705)69:1<47::aid-bit6
更新日期:2000-07-05 00:00:00
abstract::In vivo kinetics of Saccharomyces cerevisiae are studied, in a time window of 150 s, by analyzing the response of O(2) and CO(2) in the fermentor off-gas after perturbation of chemostat cultures by metabolite pulses. Here, a new mathematical method is presented for the estimation of the in vivo oxygen uptake rate (OUR...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10480
更新日期:2003-02-20 00:00:00
abstract::Lipases are versatile biocatalists showing multiple applications in a wide range of biotechnological processes. The gene lipA coding for Lipase A from Bacillus subtilis was isolated by PCR amplification, cloned and expressed in Escherichia coli, Saccharomyces cerevisiae and Bacillus subtilis strains, using pBR322, YEp...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10201
更新日期:2002-05-05 00:00:00
abstract::For direct and on-line study of the physiological states of cell cultures, a robust flow injection system has been designed and interfaced with flow cytometry (FI-FCM). The core of the flow injection system includes a microchamber designed for sample processing. The design of this microchamber allows not only an accur...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990305)62:5<609::aid-bit
更新日期:1999-03-05 00:00:00
abstract::Nitrifying bacteria, cyanobacteria, and algae are important microorganisms in open pond wastewater treatment systems. Nitrification involving the sequential oxidation of ammonia to nitrite and nitrate, mainly due to autotrophic nitrifying bacteria, is essential to biological nitrogen removal in wastewater and global n...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22860
更新日期:2010-12-15 00:00:00
abstract::This study describes the development and application of a bioaffinity chromatographic system for the one-step purification of an NADP(+)-dependent secondary alcohol dehydrogenase from the obligate anaerobe, Thermoanaerobacter brockii (TBADH, EC 1.1.1.2). The general approach is based upon improving the selectivity of ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10079
更新日期:2002-03-05 00:00:00
abstract::The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and rem...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260480508
更新日期:1995-12-05 00:00:00
abstract::This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970720)55:2<317::AID-BIT
更新日期:1997-07-20 00:00:00
abstract::The Ff filamentous bacteriophages show potential as a new class of therapeutics, displaying utility in materials science as well as pharmaceutical applications. These phages are produced by the infection of E. coli, a Gram-negative bacterium which unavoidably sheds endotoxins into the extracellular space during growth...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25571
更新日期:2015-08-01 00:00:00
abstract::High throughput pretreatment (HTPH) and enzymatic hydrolysis systems are now vital for screening large numbers of biomass samples to investigate biomass recalcitrance over various pretreatment and enzymatic hydrolysis conditions. Although hydrothermal pretreatment is currently being employed in most high throughput ap...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24951
更新日期:2013-11-01 00:00:00
abstract::Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260350309
更新日期:1990-02-05 00:00:00
abstract::A computer-aided methodology is developed for on-line monitoring and control of cell growth in fed-batch penicillin fermentation using a semidefined medium containing low corn steep liquor concentration (5.7 g/L). Cell growth is monitored and controlled with the use of experimental correlation and carbon-balancing equ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250118
更新日期:1983-01-01 00:00:00
abstract::Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21095
更新日期:2007-02-01 00:00:00
abstract::DNA/polyethylenimine (PEI) polyplexes are an important class of nonviral vectors. Although the conventional preparation method, bulk mixing, is straightforward, the formation of the DNA/PEI polyplexes is not well controlled. This work explores coaxial electrohydrodynamic spraying (EHDS) as a novel, alternative method ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22583
更新日期:2010-03-01 00:00:00
abstract::A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26249
更新日期:2017-07-01 00:00:00
abstract::The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocataly...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20885
更新日期:2006-07-05 00:00:00
abstract::The enzymatic synthesis of polyesters from activated diesters and diols has been investigated. Differences between enzymatic synthesis and traditional chemical condensation processes are discussed. The disappearance of monomers during the initial phase of reaction indicates that enzyme has a higher specificity for tra...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970705)55:1<227::AID-BIT
更新日期:1997-07-05 00:00:00
abstract::This study investigates the scaling of photobioreactor productivity based on the growth of Nannochloropsis salina incorporating the effects of direct and diffuse light. The scaling and optimization of photobioreactor geometry was analyzed by determining the growth response of a small-scale system designed to represent...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23324
更新日期:2012-02-01 00:00:00
abstract::A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB(-)) to the cells. This method can also be used for the analysis of a complex population ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(2000)71:3<208::aid-bit1010>3.0.c
更新日期:2000-01-01 00:00:00
abstract::The purpose of this article is to provide a few concrete examples of the potential to acceptably reduce the scope of validation and qualification testing based on scientific justification for the specific area of microbial fermentation. The key areas explored include: autoclave operational qualification (OQ) testing, ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1094
更新日期:2001-07-05 00:00:00
abstract::Reduced downstream costs, together with high purity recovery of polyhydroxyalkanoate (PHA), will accelerate the commercialization of high quality PHA-based products. In this work, a process was designed for effective recovery of the copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) (P(HB-co-HHx)) containing high lev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24713
更新日期:2013-02-01 00:00:00
abstract::Neural stem cells (NSCs) are undifferentiated, primitive cells with important potential applications including the replacement of neural tissue lost due to neurodegenerative diseases, including Parkinson's disease, as well as brain and spinal cord injuries, including stroke. We have developed methods to rapidly expand...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20246
更新日期:2004-11-05 00:00:00
abstract::This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24483
更新日期:2012-08-01 00:00:00
abstract::There are a series of examples in which phenols appear as contaminants in process streams and their selective removal is required for waste minimization. For the selective removal of a phenol from a mixture, we are exploiting the substrate specificity of the enzyme tyrosinase to convert phenols into reactive o-quinone...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960705)51:1<79::AID-BIT9
更新日期:1996-07-05 00:00:00
abstract::The effects of growing Clostridium acetobutylicum NCIB 8052 in a chemostat under conditions of glucose, NH(4) (+), PO(4) (3-), Mg(2+), and Fe(2+) limitation were examined. It was noted that limitation of any major nutrient resulted in the same fermentation pattern. Conditions where minor nutrient levels were reduced a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290315
更新日期:1987-02-01 00:00:00
abstract::Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a co...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20555
更新日期:2005-09-30 00:00:00
abstract::Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed)...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10099
更新日期:2001-12-01 00:00:00
abstract::In this study it is reported the operation of a horizontal-flow anaerobic immobilized biomass (HAIB) reactor under sulfate-reducing condition which was also exposed to different amounts of ethanol and toluene. The system was inoculated with sludge taken from up-flow anaerobic sludge blanket (UASB) reactors treating re...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20509
更新日期:2005-07-20 00:00:00
abstract::Cost-effective production of biopharmaceuticals on a large scale can be carried out by perfusion cultures of mammalian cells. One problem with this mode of operation for submerged free-cell cultures is the requirement for an efficient cell separation device located in the effluent stream. The present work investigates...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970505)54:3<239::AID-BIT
更新日期:1997-05-05 00:00:00
abstract::The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21013
更新日期:2006-08-20 00:00:00