Design of reactive porous polymer supports for high throughput bioreactors: poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide- co-ethylene dimethacrylate) monoliths.

Abstract:

:Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlactone functional groups. Optimization of hydrophilic/hydrophobic properties of the monolith affords a support that does not shrink in water and leads to immobilized enzyme that shows high activity in the hydrolysis of both low and high molecular weight substrates such as L-benzoyl arginine ethyl ester and casein. The catalytic activity of the monolithic reactor is maintained even at a flow velocity of 180 cm/min, which substantially exceeds those reported in the literature for packed bed reactors.

journal_name

Biotechnol Bioeng

authors

Xie S,Svec F,Fréchet JM

doi

10.1002/(sici)1097-0290(19990105)62:1<30::aid-bit4

subject

Has Abstract

pub_date

1999-01-05 00:00:00

pages

30-5

issue

1

eissn

0006-3592

issn

1097-0290

pii

10.1002/(SICI)1097-0290(19990105)62:1<30::AID-BIT4

journal_volume

62

pub_type

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