New reaction system for hydrocarbon oxidation by chloroperoxidase.


:A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfosuccinate. The emulsion system attenuated not only the destabilizing effects of the substrate and product on the enzyme by emulsifying the compounds, but also oxidant toxicity (oxidative stress) by increasing substrate availability. As a result, CPO exhibited total turnover numbers (TTNs, defined as the amount of product produced over the catalytic lifetime of the enzyme) of ca. 20,000 mol product/mol enzyme for the oxidation of styrene, toluene, and o-, m-, p-xylenes. The TTNs are over 10-fold higher than those previously reported for the oxidation of benzylic hydrocarbons by CPO. This study represents a significant step toward the development of CPO as a practical catalyst for large-scale organic syntheses.


Biotechnol Bioeng


Park JB,Clark DS




Has Abstract


2006-05-05 00:00:00












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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dols M,Remaud-Simeon M,Willemot RM,Demuth B,Jordening HJ,Buchholz K,Monsan P

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sivakumar G,Liu C,Towler MJ,Weathers PJ

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhou K,Zou R,Zhang C,Stephanopoulos G,Too HP

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chen Y,Zhou S,Cadman J,Li Q

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

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    journal_title:Biotechnology and bioengineering

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Cheah LT,Fritsch I,Haswell SJ,Greenman J

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    更新日期:2021-01-01 00:00:00