Abstract:
:Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of approximately 500 microm in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of < or =42 microm in diameter led to the dispersion of the C. fumago mycelia up to the level of single hyphae. Under these conditions the maximum specific productivity of CPO formation was enhanced about fivefold and an accumulated CPO activity in the culture supernatant of more than 1,000 U mL(-1) was achieved after 10-12 days of cultivation. In addition, the novel cultivation method also showed a positive effect on growth characteristics of other filamentous microorganisms proven by the stimulation of single hyphae/cell formation.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Kaup BA,Ehrich K,Pescheck M,Schrader Jdoi
10.1002/bit.21713subject
Has Abstractpub_date
2008-02-15 00:00:00pages
491-8issue
3eissn
0006-3592issn
1097-0290journal_volume
99pub_type
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