Abstract:
:The encapsulin nanocompartment from Rhodococcus erythropolis N771 (Reencapsulin) was expressed and purified in wild-type and C-terminally His-tagged forms. Negative-stained transmission electron microscopy, field-flow fractionation combined with multi-angle light scattering and dynamic light scattering analyses showed that 60 Reencapsulin monomers were assembled as a spherical particle with a diameter of 28 nm. Heterogeneous guest proteins such as EGFP and firefly luciferase were packaged into the internal cavity of the Reencapsulin nanocompartment by fusing the C-terminal 37-amino-acid sequence of the R. erythropolis N771 DypB peroxidase to the C-terminus. Reencapsulin has the potential to package target proteins in its internal cavity and/or display them on its external surface, making it a feasible carrier for nanotechnology applications.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Tamura A,Fukutani Y,Takami T,Fujii M,Nakaguchi Y,Murakami Y,Noguchi K,Yohda M,Odaka Mdoi
10.1002/bit.25322subject
Has Abstractpub_date
2015-01-01 00:00:00pages
13-20issue
1eissn
0006-3592issn
1097-0290journal_volume
112pub_type
杂志文章abstract::The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity (q(Ab)) of transf...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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abstract::Murine hybridoma cells were grown in a bubble column that was inclined up to 45 degrees from vertical. Inclining the column by a few degrees separated the rising bubbles against the upper surface, leaving the bulk of the liquid bubble free. The liquid was circulated well by the rising bubbles, but collection of cells ...
journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.260420506
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journal_title:Biotechnology and bioengineering
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abstract::Membrane chromatography (MC) systems are finding increasing use in downstream processing trains for therapeutic proteins due to the unique mass-transfer characteristics they provide. As a result, there is increased need for model-based methods to scale-up MC units using data collected on a scaled-down unit. Here, a st...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25217
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260281110
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260401018
更新日期:1992-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270711
更新日期:1985-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000220)67:4<451::aid-bit
更新日期:2000-02-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21575
更新日期:2008-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260200709
更新日期:1978-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260241010
更新日期:1982-10-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22098
更新日期:2009-02-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000405)68:1<44::aid-bit5
更新日期:2000-04-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10111
更新日期:2002-01-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1997-01-20 00:00:00
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pub_type: 杂志文章
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更新日期:2003-07-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2003-10-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2019-11-01 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2007-02-15 00:00:00
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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