Abstract:
:The adsorption of Thiobacillus ferrooxidans to coal surfaces has been studied. Adsorption experiments were conducted on coal samples from eight different Eastern coal fields. In all cases the adsorption process was at least 90% complete within the first two minutes following inoculation. The results of these experiments were used to test the validity of two proposed adsorption models. The first model assumes that bacterial adsorption follows second-order irreversible kinetics of the second kind with respect to the concentration of bacteria and substratum surface area in the system. The second model allows for the contribution of reversible adsorption detected in desorption experiments. It was found that the combined reversible-irreversible model more accurately describes the initial stages of adsorption. Rate constants in both models were calculated for each coal sample. The relation of each of these constants to the pyrite concentration in coal is presented and the significance of these relations is discussed. Scanning electron micrographs of inoculated coal samples show that Thiobacillus ferrooxidans selectively adsorb to exposed pyrite phases dispersed throughout the organic coal matrix. Preferential attachment was also observed along topographical faults in the coal surface. Mercury contact angle measurements on coal indicate that the selective adsorption of Thiobacillus ferrooxidans may be attributable to the lower surface free energy of pyrite relative to the organic coal matrix.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Bagdigian RM,Myerson ASdoi
10.1002/bit.260280402subject
Has Abstractpub_date
1986-04-01 00:00:00pages
467-79issue
4eissn
0006-3592issn
1097-0290journal_volume
28pub_type
杂志文章abstract::Multivalent cations have been known to be important components of activated sludge floc structure due to their bridging ability of the negatively charged sites on the biopolymer network. Recently in batch systems it was found that excess concentration of monovalent cations led to the deterioration in settleability, de...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21755
更新日期:2008-06-01 00:00:00
abstract::Accurate measurement of enzymatic cellulose digestibility (X) is important in evaluating the efficiency of lignocellulose pretreatment technologies, assessing the performance of reconstituted cellulase mixtures, and conducting economic analysis for biorefinery processes. We analyzed the effect of sugars contained in e...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21178
更新日期:2007-01-01 00:00:00
abstract::Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19961220)52:6<713::AID-BIT
更新日期:1996-12-20 00:00:00
abstract::Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating electrochemical eff...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980820)59:4<461::aid-bit
更新日期:1998-08-20 00:00:00
abstract::Understanding the regulatory networks which control specific macrophage phenotypes is essential in identifying novel targets to correct macrophage mediated clinical disorders, often accompanied by inflammatory events. Since mesenchymal stromal cells (MSCs) have been shown to play key roles in regulating immune functio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25282
更新日期:2014-11-01 00:00:00
abstract::Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24853
更新日期:2013-07-01 00:00:00
abstract::The marine diatom Cyclotella cryptica was grown over a period of 13 months in a 48-m(2) shallow outdoor flume. The use of foil arrays at intervals of 1.2 m to effect systematic vertical mixing in the flume was found to significantly enhance microalgal production (p = 0.006). Average photosynthetic efficiencies (based ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320204
更新日期:1988-07-05 00:00:00
abstract::Liver tissue engineering aims to create transplantable liver grafts that can serve as substitutes for donor's livers. One major challenge in creating a fully functional liver tissue has been to recreate the biliary drainage in an engineered liver construct through integration of bile canaliculi (BC) with the biliary d...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27546
更新日期:2021-01-01 00:00:00
abstract::An in-situ, mid-infrared sensor was used to monitor the major analyte concentrations involved in the cultivation of Gluconacetobacter xylinus and the production of gluconacetan, a food-grade exopolysaccharide. To predict the analyte concentrations, three different sets of standard spectra were used to develop calibrat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10618
更新日期:2003-06-20 00:00:00
abstract::For all bacteria, nearly every antimicrobial fails since a subpopulation of the bacteria enter a dormant state known as persistence, in which the antimicrobials are rendered ineffective due to the lack of metabolism. This tolerance to antibiotics makes microbial infections the leading cause of death worldwide and make...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25963
更新日期:2016-09-01 00:00:00
abstract::In our preceding article, we demonstrated a procedure based upon enzymic flow microcalorimetry using an enzyme thermistor (ET) to characterize the microkinetic properties of an immobilized enzyme (IME) and its further application in the screening of IMEs. To consider the ET method (single ET unit, ET system 1) as stan...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960105)49:1<26::AID-BIT4
更新日期:1996-01-05 00:00:00
abstract::Physical damage of animal cells in suspension culture, due to stirring and sparging, is coupled with complex metabolic responses. Nylon microcapsules, therefore, were used as a physical model to study the mechanisms of damage in a stirred bioreactor and in a bubble column. Microcapsule breaskage folowed first-order ki...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260401018
更新日期:1992-12-05 00:00:00
abstract::The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-01-20 00:00:00
abstract::A comprehensive study of the application of continuous zone electrophoresis to preparative separation of proteins in free solution is presented. First, the influence of electric field strength, buffer residence time in the chamber, sample flow rate, and sample concentration on separation resolution and throughput were...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420707
更新日期:1993-09-20 00:00:00
abstract::CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10176
更新日期:2002-02-15 00:00:00
abstract::To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260470610
更新日期:1995-09-20 00:00:00
abstract::Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glyco...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26330
更新日期:2017-09-01 00:00:00
abstract::A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10793
更新日期:2003-11-20 00:00:00
abstract::We have identified an error in the published integral form of the modified Michaelis-Menten equation that accounts for endogenous substrate production. The correct solution is presented and the error in both the substrate concentration, S, and the kinetic parameters Vm , Km , and R resulting from the incorrect solutio...
journal_title:Biotechnology and bioengineering
pub_type: 信件
doi:10.1002/bit.23181
更新日期:2011-10-01 00:00:00
abstract::Trypsin inhibitor was converted to hydrophobic states by covalently combining cholesteryl groups using an acylation reaction, and was immobilized in reverse micelles composed of a nonionic surfactant. Using this reverse micellar phase containing trypsin inhibitor as an affinity ligand, trypsin was selectively separate...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970220)53:4<406::AID-BIT
更新日期:1997-02-20 00:00:00
abstract::This paper describes batch and semicontinuous acetic acid fermentations for wine vinegar production carried out with Acetobacter pasteurianus, and an industrial strain using a noncommercial 100-L bubble column reactor equipped with a novel type of gas-liquid dynamic sparger. Results showed acetification rates with thi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990420)63:2<141::aid-bit
更新日期:1999-04-20 00:00:00
abstract::Metabolite accumulation has pleiotropic, toxic, or beneficial effects on cell physiology, but such effects are not well understood at the molecular level. Cells respond and adapt to metabolite stress by mechanisms largely unexplored, especially in the context of multiple and simultaneous stresses. Solventogenic and re...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22628
更新日期:2010-04-15 00:00:00
abstract::This paper describes a novel surface immobilization method for live-cell imaging of Chlamydomonas reinhardtii for continuous monitoring of lipid droplet accumulation. Microfluidics allows high-throughput manipulation and analysis of single cells in precisely controlled microenvironment. Fluorescence imaging based quan...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25453
更新日期:2015-03-01 00:00:00
abstract::The kinetics of cellular reproduction and the rate and extent of synthesis of extracellular polymeric substances (EPS) were investigated for P. aeruginosa growing in glucose-limited chemostats. mu(max) and K(s) estimates of 0.4 h(-1) and 2 mg glucose C/L, respectively, at 25 degrees C were obtained for this bacterium....
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260261203
更新日期:1984-12-01 00:00:00
abstract::Screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that Coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. Treatment of the same effluent with this fungus, immobilized in beads...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250814
更新日期:1983-08-01 00:00:00
abstract::Lipase (EC 3.1.1.3., from Pseudomonas sp.) was entrapped in collagen membrane containing liquid crystal (4-methoxybenzilidene-4'-n-butylaniline). The activity of the lipase-liquid crystal membrane at an applied voltage of 4 V was 3.4 compared to a membrane tested without imposition of an external electric field. A lin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260191007
更新日期:1977-10-01 00:00:00
abstract::Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22409
更新日期:2009-10-15 00:00:00
abstract::The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970520)54:4<333::AID-BIT
更新日期:1997-05-20 00:00:00
abstract::Mucopolysaccharidosis (MPS) Type II is caused by mutations in the gene encoding the lysosomal enzyme, iduronate 2-sulfatase (IDS). The majority of MPSII cases affect the brain. However, enzyme replacement therapy with recombinant IDS does not treat the brain, because IDS is a large molecule drug that does not cross th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25289
更新日期:2014-11-01 00:00:00
abstract::We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displac...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21913
更新日期:2008-10-15 00:00:00