Physical modeling of animal cell damage by hydrodynamic forces in suspension cultures.

Abstract:

:Physical damage of animal cells in suspension culture, due to stirring and sparging, is coupled with complex metabolic responses. Nylon microcapsules, therefore, were used as a physical model to study the mechanisms of damage in a stirred bioreactor and in a bubble column. Microcapsule breaskage folowed first-order kinetices in all experiments Entrainment of bubbles into the liquid phase in the stirred bioreactor gave more microcapsule breakage. In the bubble column, the bubble bursting zone at gas-liquid interface was primarily responsible for microcapsule breakage. The forces on the microcapsules were equivalent to an external pressure of approximately 4 x 10(4) N. m(-2), based on the critical microcapsule diameter for survival of 190 microm. A stable foam layer, however, was found to be effective in protecting microcapsules from damage. The microcapsule transport to the gas-liquid interface and entrainment into the foam phase was consistent with flotation by air bubbles. This result implies that additives and operation of bioreactors should be selected to minimize flotation of cells.

journal_name

Biotechnol Bioeng

authors

Lu GZ,Gray MR,Thompson BG

doi

10.1002/bit.260401018

subject

Has Abstract

pub_date

1992-12-05 00:00:00

pages

1277-81

issue

10

eissn

0006-3592

issn

1097-0290

journal_volume

40

pub_type

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