Cellular reporoduction and extracellular polymer formation by Pseudomonas aeruginosa in continuous culture.

Abstract:

:The kinetics of cellular reproduction and the rate and extent of synthesis of extracellular polymeric substances (EPS) were investigated for P. aeruginosa growing in glucose-limited chemostats. mu(max) and K(s) estimates of 0.4 h(-1) and 2 mg glucose C/L, respectively, at 25 degrees C were obtained for this bacterium. The extent of EPS formation was inversely related to the growth rate of P. aeruginosa. The rate of EPS formation had both growth- and non-growth-associated components. The growth-associated polymer formation rate coefficient (k) was 0.3 mg polymer C/mg cellular C and the non-growth-associated polymer formation rate coefficient (k') was 0.04 mg polymer C/mg cellular C/h. The values for k and k' must be regarded as provisional since the product formation data were quite variable at low dilution rates. Estimates of the cellular (Y(x/s)) and polymer (Y(p/s)) yield coefficients were 0.3 mg cellular C/mg glucose C and 0.6 mg polymer C/mg glucose C, respectively. Most of the non-growth-associated consumption of glucose detected was due to exopolymer formation.

journal_name

Biotechnol Bioeng

authors

Robinson JA,Trulear MG,Characklis WG

doi

10.1002/bit.260261203

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

1409-17

issue

12

eissn

0006-3592

issn

1097-0290

journal_volume

26

pub_type

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