Cellular reporoduction and extracellular polymer formation by Pseudomonas aeruginosa in continuous culture.


:The kinetics of cellular reproduction and the rate and extent of synthesis of extracellular polymeric substances (EPS) were investigated for P. aeruginosa growing in glucose-limited chemostats. mu(max) and K(s) estimates of 0.4 h(-1) and 2 mg glucose C/L, respectively, at 25 degrees C were obtained for this bacterium. The extent of EPS formation was inversely related to the growth rate of P. aeruginosa. The rate of EPS formation had both growth- and non-growth-associated components. The growth-associated polymer formation rate coefficient (k) was 0.3 mg polymer C/mg cellular C and the non-growth-associated polymer formation rate coefficient (k') was 0.04 mg polymer C/mg cellular C/h. The values for k and k' must be regarded as provisional since the product formation data were quite variable at low dilution rates. Estimates of the cellular (Y(x/s)) and polymer (Y(p/s)) yield coefficients were 0.3 mg cellular C/mg glucose C and 0.6 mg polymer C/mg glucose C, respectively. Most of the non-growth-associated consumption of glucose detected was due to exopolymer formation.


Biotechnol Bioeng


Robinson JA,Trulear MG,Characklis WG




Has Abstract


1984-12-01 00:00:00












  • Operational stability of copolymerized enzymes at elevated temperatures.

    abstract::The copolymerization method of immobilization was used to obtain preparations of enzymes covalently incorporated in polyacrylamide gel. They possess properties making them suitable for practical use. First, the preparations are hundreds of times more stable against irreversible thermoinactivation than native enzymes. ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mozhaev VV,Siksnis VA,Torchilin VP,Martinek K

    更新日期:1983-08-01 00:00:00

  • Modeling and analysis of radial flow mammalian cell culture.

    abstract::A model for radial flow mammalian cell culture has been developed. Two situations, one with permeable hollow fibers and the other with a porous matrix in the annulus, were considered. The hollow fibers were modeled as continuously distributed sinks. Numerical solutions are presented for the complete model as well as l...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tharakan JP,Chau PC

    更新日期:1987-04-01 00:00:00

  • Fractional precipitation of plasmid DNA from lysate by CTAB.

    abstract::Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lander RJ,Winters MA,Meacle FJ,Buckland BC,Lee AL

    更新日期:2002-09-30 00:00:00

  • Co-culturing Chlorella minutissima with Escherichia coli can increase neutral lipid production and improve biodiesel quality.

    abstract::Lipid productivity and fatty acid composition are important metrics for the production of high quality biodiesel from algae. Our previous results showed that co-culturing the green alga Chlorella minutissima with Escherichia coli under high-substrate mixotrophic conditions enhanced both culture growth and crude lipid ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Higgins BT,Labavitch JM,VanderGheynst JS

    更新日期:2015-09-01 00:00:00

  • Monitoring of dissolved oxygen and cellular bioenergetics within a pancreatic substitute.

    abstract::This work investigated the use of nuclear magnetic resonance (NMR) spectroscopy in combination with a mathematical model of an encapsulated cell system as a method for rapidly assessing the status of a pancreatic substitute. To validate this method, an in vitro experiment was performed in which the encapsulated cells ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gross JD,Long RC Jr,Constantinidis I,Sambanis A

    更新日期:2007-09-01 00:00:00

  • Elucidation of enzyme control mechanisms using macroscopic measurements in a mixed substrate fermentation system.

    abstract::The objective of this work was to relate macroscopically measurable on-line fermentation parameters such as dissolved oxygen, off-gas oxygen and carbon dioxide, and cell mass, to the controlled production of key intracellular enzymes under carbon limited conditions. Both batch and perturbed batch aerobic fermentations...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pih N,De Bernardez E,Dhurjati P

    更新日期:1988-03-01 00:00:00

  • Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture.

    abstract::Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim Y,Arp DJ,Semprini L

    更新日期:2002-12-05 00:00:00

  • Enzymatic coupling of phenol vapors onto chitosan.

    abstract::Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed)...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wu LQ,Chen T,Wallace KK,Vazquez-Duhalt R,Payne GF

    更新日期:2001-12-01 00:00:00

  • Model-based derivation, analysis and control of unstable microaerobic steady-states--considering Rhodospirillum rubrum as an example.

    abstract::Microaerobic (oxygen-limited) conditions are critical for inducing many important microbial processes in industrial or environmental applications. At very low oxygen concentrations, however, the process performance often suffers from technical limitations. Available dissolved oxygen measurement techniques are not sens...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Carius L,Rumschinski P,Faulwasser T,Flockerzi D,Grammel H,Findeisen R

    更新日期:2014-04-01 00:00:00

  • Characterization of free and alginate-polylysine-alginate microencapsulated Erwinia herbicola for the conversion of ammonia, pyruvate, and phenol into L-tyrosine.

    abstract::The whole cell tyrosine phenol-lyase (TPL, E.C. activity of Erwinia herbicola (ATCC 21434) was microen-capsulated. We studied the use of this for the conversion of ammonia and pyruvate along with phenol or catechol, respectively, into L-tyrosine or dihydroxyphenyl-L-alanine (L-dopa). The reactions are releva...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lloyd-George I,Chang TM

    更新日期:1995-12-20 00:00:00

  • NADH and flavin fluorescence responses of starved yeast cultures to substrate additions.

    abstract::Model experiments were performed with starved yeast (Saccharomyces cerevisiae) cultures in a batch reactor in order to develop a better understanding of NAD(P)H and flavin culture fluorescence. Fluorescence was monitored during aerobic-anaerobic-aerobic transitions and ethanol and glucose substrate addition experiment...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Siano SA,Mutharasan R

    更新日期:1989-08-20 00:00:00

  • Alginate microparticles as novel carrier for oral insulin delivery.

    abstract::Alginate microparticles produced by emulsification/internal gelation were investigated as a promising carrier for insulin delivery. The procedure involves the dispersion of alginate solution containing insulin protein, into a water immiscible phase. Gelation is triggered in situ by instantaneous release of ionic calci...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Reis CP,Ribeiro AJ,Neufeld RJ,Veiga F

    更新日期:2007-04-01 00:00:00

  • Progression of continuous downstream processing of monoclonal antibodies: Current trends and challenges.

    abstract::Rapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and flexibility, reduce footprint and cost of goods, and improve product...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Somasundaram B,Pleitt K,Shave E,Baker K,Lua LHL

    更新日期:2018-12-01 00:00:00

  • Selection and initial characterization of novel peptide ligands that bind specifically to human blood outgrowth endothelial cells.

    abstract::Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Veleva AN,Cooper SL,Patterson C

    更新日期:2007-09-01 00:00:00

  • Photo inactivation of virus particles in microfluidic capillary systems.

    abstract::It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ren Y,Crump CM,Mackley MM,Li Puma G,Reis NM

    更新日期:2016-07-01 00:00:00

  • Optimization of microalgal production in a shallow outdoor flume.

    abstract::The marine diatom Cyclotella cryptica was grown over a period of 13 months in a 48-m(2) shallow outdoor flume. The use of foil arrays at intervals of 1.2 m to effect systematic vertical mixing in the flume was found to significantly enhance microalgal production (p = 0.006). Average photosynthetic efficiencies (based ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Laws EA,Taguchi S,Hirata J,Pang L

    更新日期:1988-07-05 00:00:00

  • A novel carbonochloridate for activation of supports containing hydroxyl groups.

    abstract::The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5-norbornene-2.3-dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Büttner W,Becker M,Rupprich C,Boeden HF,Henklein P,Loth F,Dautzenberg H

    更新日期:1989-01-05 00:00:00

  • Non-invasive mass transfer measurements in complex biofilm-coated structures.

    abstract::We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displac...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Graf von der Schulenburg DA,Akpa BS,Gladden LF,Johns ML

    更新日期:2008-10-15 00:00:00

  • Growth of Leuconostoc mesenteroides NRRL-B523 in an alkaline medium: suboptimal pH growth inhibition of a lactic acid bacterium.

    abstract::Bacterial profile modification (BPM), a form of tertiary oil recovery, diverts water from the water-flooded high-permeability zone into the oil-bearing low-permeability zone. During field use, exopolymer-producing bacteria plug the high-permeability zone only in the immediate vicinity of the injection point (the near-...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wolf BF,Fogler HS

    更新日期:2005-01-05 00:00:00

  • Salt-induced changes in plasmid DNA transmission through ultrafiltration membranes.

    abstract::Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Latulippe DR,Zydney AL

    更新日期:2008-02-01 00:00:00

  • Strategy for selection of methods for separation of bioparticles from particle mixtures.

    abstract::The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocataly...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: van Hee P,Hoeben MA,van der Lans RG,van der Wielen LA

    更新日期:2006-07-05 00:00:00

  • Metabolite stress and tolerance in the production of biofuels and chemicals: gene-expression-based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum.

    abstract::Metabolite accumulation has pleiotropic, toxic, or beneficial effects on cell physiology, but such effects are not well understood at the molecular level. Cells respond and adapt to metabolite stress by mechanisms largely unexplored, especially in the context of multiple and simultaneous stresses. Solventogenic and re...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Alsaker KV,Paredes C,Papoutsakis ET

    更新日期:2010-04-15 00:00:00

  • Culture of insect cells in helical ribbon impeller bioreactor.

    abstract::An 11-L helical ribbon impeller (HRI) bioreactor was tested for the culture of Spodoptera frugiperda (Sf-9) cells. This impeller and surface baffling ensured homogeneous mixing and high oxygen transfer through surface aeration and surface-induced babble generation. Serum-supplemented and serum-free cultures, using TNM...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kamen AA,Tom RL,Caron AW,Chavarie C,Massie B,Archambault J

    更新日期:1991-09-01 00:00:00

  • Kinetic analysis of bacterial bioluminescence.

    abstract::Bioluminescence from the lux-based bacterial reporter Pseudomonas fluorescens HK44 was experimentally investigated under growth substrate-rich and limiting conditions in batch, continuous stirred tank (CSTR), and turbidostat reactors. A mechanistically based, mathematical model was developed to describe bioluminescenc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kelly CJ,Hsiung CJ,Lajoie CA

    更新日期:2003-02-05 00:00:00

  • Effect of activation energy microheterogeneity on first-order enzyme deactivation.

    abstract::The influence of microheterogeneity on enzyme inactivation kinetics is examined. A continuous normal distribution of the thermal activation energy is assumed, and using this, a simple mathematical model is developed to find the activity-time trajectories for a microheterogeneous enzyme. Using an example, the model is ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Malhotra A,Sadana A

    更新日期:1987-07-01 00:00:00

  • A general kinetic model for biological nutrient removal activated sludge systems: model development.

    abstract::In this article, a kinetic model is developed and presented for biological nutrient removal (BNR) activated sludge (BNRAS) systems in general, but for external nitrification (EN) BNRAS (ENBNRAS) systems in particular. The model is based on the UCTPHO model, but includes some significant modifications, such as anoxic P...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hu ZR,Wentzel MC,Ekama GA

    更新日期:2007-12-15 00:00:00

  • Combination drug delivery via multilamellar vesicles enables targeting of tumor cells and tumor vasculature.

    abstract::Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Liu Y,Kim YJ,Siriwon N,Rohrs JA,Yu Z,Wanga P

    更新日期:2018-06-01 00:00:00

  • Recovery of insect cells using hollow fiber microfiltration.

    abstract::An efficient method was developed for media separation and cell collection for eukaryotic cells growing in suspension. The method is based on tangential flow microfiltration using an open channel arrangement in a hollow fiber configuration. Best results (highest processing flux rate) for polysulfone hollow fibers were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Trinh L,Shiloach J

    更新日期:1995-11-20 00:00:00

  • A novel recycle batch immobilized cell bioreactor for propionate production from whey lactose.

    abstract::Recycle batch fermentations using immobilized cells of Propionibacterium acidipropionici were studied for propionate production from whey permeate, de-lactose whey permeate, and acid whey. Cells were immobilized in a spirally wound fibrous sheet packed in a 0.5-L column reactor, which was connected to a 5-L stirred ta...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yang ST,Huang Y,Hong G

    更新日期:1995-03-05 00:00:00

  • Characterization of a three bacteria mixed culture in a chemostat: evaluation and application of a quantitative terminal-restriction fragment length polymorphism (T-RFLP) analysis for absolute and species specific cell enumeration.

    abstract::Growth dynamics of Pseudomonas aeruginosa, Burkholderia cepacia, and Staphylococcus aureus in a batch and chemostat, were investigated as a laboratory model system for persistent infections in cystic fibrosis. Most species-specific enumeration methods for mixed cultures are laborious or only qualitative, and therefore...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schmidt JK,König B,Reichl U

    更新日期:2007-03-01 00:00:00