Cellular reporoduction and extracellular polymer formation by Pseudomonas aeruginosa in continuous culture.

abstract：:A key point of protein stability engineering is to identify specific target residues whose mutations can stabilize the protein structure without negatively affecting the function or activity of the protein. Here, we propose a method called RiSLnet (Rapid identification of Smart mutant Library using residue network) to...

journal_title：Biotechnology and bioengineering

authors： Upadhyay R,Kim JY,Hong EY,Lee SG,Seo JH,Kim BG

更新日期：2019-02-01 00:00:00

abstract：:The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...

journal_title：Biotechnology and bioengineering

authors： Shimizu H,Araki K,Shioya S,Suga K

更新日期：1991-06-20 00:00:00

abstract：:It is a common practice in biotherapeutic manufacturing to define a fixed-volume feed strategy for nutrient feeds, based on historical cell demand. However, once the feed volumes are defined, they are inflexible to batch-to-batch variations in cell growth and physiology and can lead to inconsistent productivity and pr...

journal_title：Biotechnology and bioengineering

authors： Zhang A,Tsang VL,Moore B,Shen V,Huang YM,Kshirsagar R,Ryll T

更新日期：2015-12-01 00:00:00

abstract：:Mixed-culture fermentation is a key central process to enable next generation biofuels and biocommodity production due to economic and process advantages over application of pure cultures. However, a key limitation to the application of mixed-culture fermentation is predicting culture product response, related to meta...

journal_title：Biotechnology and bioengineering

authors： Hoelzle RD,Virdis B,Batstone DJ

更新日期：2014-11-01 00:00:00

abstract：:The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. Th...

journal_title：Biotechnology and bioengineering

authors： Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GS

更新日期：1997-06-05 00:00:00

abstract：:Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed)...

journal_title：Biotechnology and bioengineering

authors： Wu LQ,Chen T,Wallace KK,Vazquez-Duhalt R,Payne GF

更新日期：2001-12-01 00:00:00

abstract：:Hydroxycinnamic acids are known to inhibit microbial growth during fermentation of lignocellulosic biomass hydrolysates, and the ability to diminish hydroxycinnamic acid toxicity would allow for more effective biological conversion of biomass to fuels and other value-added products. In this work, we provide a proof-of...

journal_title：Biotechnology and bioengineering

authors： Tomek KJ,Saldarriaga CR,Velasquez FP,Liu T,Hodge DB,Whitehead TA

更新日期：2015-03-01 00:00:00

abstract：:The production of dry-grind corn ethanol results in the generation of intermediate products, thin and whole stillage, which require energy-intensive downstream processing for conversion into commercial animal feed products. Hydrothermal carbonization of thin and whole stillage coupled with anaerobic digestion was inve...

journal_title：Biotechnology and bioengineering

authors： Wood BM,Jader LR,Schendel FJ,Hahn NJ,Valentas KJ,McNamara PJ,Novak PM,Heilmann SM

更新日期：2013-10-01 00:00:00

abstract：:Application of the well-characterized Streptococcus pyogenes CRISPR-Cas9 system in actinomycetes streptomycetes has enabled high-efficiency multiplex genome editing and CRISPRi-mediated transcriptional regulation in these prolific bioactive metabolite producers. Nonetheless, SpCas9 has its limitations and can be ineff...

journal_title：Biotechnology and bioengineering

authors： Yeo WL,Heng E,Tan LL,Lim YW,Lim YH,Hoon S,Zhao H,Zhang MM,Wong FT

更新日期：2019-09-01 00:00:00

abstract：:Rate and yield expressions relating to biomass and xanthan formation and to nitrogen, glucose, and oxygen consumption were established for Xanthomonas campestris batch fermentations in a bubble column. Microbial growth was described by the logistic rate equation, characterized by a maximum specific growth rate mu(M) =...

journal_title：Biotechnology and bioengineering

authors： Pons A,Dussap CG,Gros JB

更新日期：1989-01-20 00:00:00

abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...

journal_title：Biotechnology and bioengineering

authors： Cremonesi P,Carrea G,Ferrara L,Antonini E

更新日期：1975-08-01 00:00:00

abstract：:A bioremediation process to remove Co(2+) from aqueous solution is investigated in this study using a magnetic resonance imaging (MRI) protocol to rapidly obtain multiple 2D spatially resolved Co(2+) ion concentration maps. The MRI technique is described in detail and its ability to determine the evolution in both axi...

journal_title：Biotechnology and bioengineering

authors： Graf von der Schulenburg DA,Holland DJ,Paterson-Beedle M,Macaskie LE,Gladden LF,Johns ML

更新日期：2008-03-01 00:00:00

abstract：:Several experimental data on continuous cultures of hybridoma cells show that monoclonal antibody productivity is a decreasing function of dilution rate. It has been suggested that this unusual behavior may be due to the arrest of a fraction of cycling cells at a critical point of Phase G(1). Although this hypothesis ...

journal_title：Biotechnology and bioengineering

authors： Cazzador L,Mariani L

更新日期：1993-12-01 00:00:00

abstract：:Protein dynamic binding capacities on ion exchange resins are typically expected to decrease with increasing conductivity and decreasing protein charge. There are, however, conditions where capacity increases with increasing conductivity and decreasing protein charge. Capacity measurements on two different commercial ...

journal_title：Biotechnology and bioengineering

authors： Harinarayan C,Mueller J,Ljunglöf A,Fahrner R,Van Alstine J,van Reis R

更新日期：2006-12-05 00:00:00

abstract：:Surface modified bioactive glass with surface properties akin to those of the bone mineral phase is an attractive candidate for use as a microcarrier material for 3-D growth of bone-like tissue in rotating wall vessel bioreactors (RWVs). The critical surface properties of this material are the result of reaction in so...

journal_title：Biotechnology and bioengineering

authors： Radin S,Ducheyne P,Ayyaswamy PS,Gao H

更新日期：2001-11-05 00:00:00

abstract：:Soluble steel factor (SF) is a potent stimulator of hematopoietic progenitor cell proliferation in vitro, and cytokine combinations that include SF can support extensive expansions of hematopoietic cells. Recently, we showed that very primitive progenitor cells from normal human bone marrow require exposure to very hi...

journal_title：Biotechnology and bioengineering

authors： Zandstra PW,Jervis E,Haynes CA,Kilburn DG,Eaves CJ,Piret JM

更新日期：1999-05-20 00:00:00

abstract：:Experimental and theoretical studies are conducted to understand the dynamic behavior of a continuous-flow fixed-bed reactor in which an esterification is catalyzed by an immobilized enzyme in an organic solvent medium. The experimental system consists of a commercial immobilized lipase preparation known as Lipozyme a...

journal_title：Biotechnology and bioengineering

authors： Mensah P,Gainer JL,Carta G

更新日期：1998-11-20 00:00:00

abstract：:Insulin is an important biotherapeutic protein, and it is also a model protein used to study amyloid diseases, such as Alzheimer's and Parkinson's. The preparation of the protein can lead to small amounts of aggregate in the solution, which in turn may lead to irreproducible in vitro results. Using several pre-treatme...

journal_title：Biotechnology and bioengineering

authors： Heldt CL,Sorci M,Posada D,Hirsa A,Belfort G

更新日期：2011-01-01 00:00:00

abstract：:Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...

journal_title：Biotechnology and bioengineering

authors： Bohle K,Ross A

更新日期：2011-09-01 00:00:00

abstract：:The release kinetics of beta-galactosidase protein have been determined during small-scale ultrasonication of E. coli cells. Among several studied parameters, ionic strength and cell concentration have the least influence on the rate of protein recovery, whereas sample volume and acoustic power dramatically affect the...

journal_title：Biotechnology and bioengineering

authors： Feliu JX,Cubarsi R,Villaverde A

更新日期：1998-06-05 00:00:00

abstract：:Sugarcane bagasse is an abundant and sustainable resource, generated as a by-product of sugarcane milling. The cellulosic material within bagasse can be broken down into glucose molecules and fermented to produce ethanol, making it a promising feedstock for biofuel production. Mild acid pretreatment hydrolyses the hem...

journal_title：Biotechnology and bioengineering

authors： Greenwood AA,Farrell TW,O'Hara IM

更新日期：2013-12-01 00:00:00

abstract：:Product inhibition of biological production systems is a widely observed phenomenon with prominent implications for the design and ultimately the success of biotechnological processes. In order to investigate whether such effects could limit the maximal concentrations in the production of the beta-lactamase inhibitor ...

journal_title：Biotechnology and bioengineering

authors： Brethauer S,Held M,Panke S

更新日期：2008-06-15 00:00:00

abstract：:A novel feeding strategy that prolongs rapid growth rates for Escherichia coli fermentations to moderately high cell density is presented. High-density fermentations are a common and successful means of producing biological products. However, acetate accumulation can be a substantial problem in these procedures. To av...

journal_title：Biotechnology and bioengineering

authors： Zawada J,Swartz J

更新日期：2005-02-20 00:00:00

abstract：:Cultures with immobilized hybridoma cells were performed in fixed bed systems. "Steady state" values for volume-specific substrate uptake and metabolite production rates were determined at various perfusion rates and superficial flow velocities of the medium within the carrier matrix. Data from fixed bed volumes betwe...

journal_title：Biotechnology and bioengineering

authors： Pörtner R,Rössing S,Koop M,Lüdemann I

更新日期：1997-08-05 00:00:00

abstract：:A model was developed to evaluate the effects of cells and surfactants on oxygen transfer in surface-aerated bioreactors. The model assumed the presence of serial layers of adsorbed surfactants and microorganisms directly adjacent to the gas-liquid interface due to their surface activities, followed by a stagnant liqu...

journal_title：Biotechnology and bioengineering

authors： Ju LK,Sundarajan A

更新日期：1992-12-20 00:00:00

abstract：:Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to re...

journal_title：Biotechnology and bioengineering

authors： Wu-Fienberg Y,Moore AM,Marquardt LM,Newton P,Johnson PJ,Mackinnon SE,Sakiyama-Elbert SE,Wood MD

更新日期：2014-09-01 00:00:00

abstract：:A second generation flow contraction device was developed and modeled which allows cells to be subjected to well-defined hydrodynamic forces. Studies were conducted with this system on wild-type Chinese Hamster Ovary cells (CHO-K1) and a strain of CHO cells which expresses the human Bcl-2 triangle gene (CHO-bcl-2). In...

journal_title：Biotechnology and bioengineering

authors： Mollet M,Godoy-Silva R,Berdugo C,Chalmers JJ

更新日期：2007-11-01 00:00:00

abstract：:The application of the unscented Kalman filter to control starvation-induced programmed cell death-apoptosis-in Chinese hamster ovary cells was investigated. Neural network-based sensitivity analysis identified glutamine and asparagine as two major amino acids that play a key role in the suppression of apoptosis. Dyna...

journal_title：Biotechnology and bioengineering

authors： Simon L,Karim MN

更新日期：2002-06-20 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:Recycle batch fermentations using immobilized cells of Propionibacterium acidipropionici were studied for propionate production from whey permeate, de-lactose whey permeate, and acid whey. Cells were immobilized in a spirally wound fibrous sheet packed in a 0.5-L column reactor, which was connected to a 5-L stirred ta...

journal_title：Biotechnology and bioengineering

authors： Yang ST,Huang Y,Hong G

更新日期：1995-03-05 00:00:00