Abstract:
:The sec-alcohol dehydrogenase activity of whole cells of Rhodococcus ruber DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH/NAD(+) can be omitted. The operational half-life time of the redox system is 29 hours in 20% v/v acetone and 37 hours in 30% v/v 2-propanol. The Redox system allows the enantioselective oxidation of sec-alcohols and the asymmetric reduction of ketones to furnish (S)-configurated alcohols in high optical purity. The stability of the cells towards further organic solvents was investigated. In addition, the system displays thermostability of up to 60 degrees C and pH stability of up to pH 11. The system represents a simple to handle tool for environmentally benign redox reactions.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Stampfer W,Kosjek B,Kroutil W,Faber Kdoi
10.1002/bit.10540subject
Has Abstractpub_date
2003-03-30 00:00:00pages
865-9issue
7eissn
0006-3592issn
1097-0290journal_volume
81pub_type
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