Abstract:
:Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed) onto chitosan films. First, phenol-trapping studies indicated that p-cresol can be removed from vapors if the vapors are contacted with tyrosinase-coated chitosan films. Second, the ultraviolet absorbance of tyrosinase-coated chitosan films changes dramatically when they are contacted with cresol-containing vapors, whereas control films are unaffected by contacting with cresol vapors. Third, pressure measurements indicate that tyrosinase-coated chitosan films only react with cresol vapors if the oxygen cosubstrate is present. Additional studies demonstrate the potential of tyrosinase-coated chitosan films/membranes for the detection and removal of phenol vapors.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Wu LQ,Chen T,Wallace KK,Vazquez-Duhalt R,Payne GFdoi
10.1002/bit.10099subject
Has Abstractpub_date
2001-12-01 00:00:00pages
325-32issue
4eissn
0006-3592issn
1097-0290pii
10.1002/bit.10099journal_volume
76pub_type
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