Engineering of baker's yeasts, E. coli and Bacillus hosts for the production of Bacillus subtilis Lipase A.

abstract：:Kluyveromyces marxianus is a promising nonconventional yeast for biobased chemical production due to its rapid growth rate, high TCA cycle flux, and tolerance to low pH and high temperature. Unlike Saccharomyces cerevisiae, K. marxianus grows on low-cost substrates to cell densities that equal or surpass densities in ...

journal_title：Biotechnology and bioengineering

authors： McTaggart TL,Bever D,Bassett S,Da Silva NA

更新日期：2019-07-01 00:00:00

abstract：:Tissue engineering devices as in vitro cell culture systems in scaffolds has encountered the bottleneck due to their much lower cell functions than real tissues/organs in vivo. Such situation has been improved in some extent by mimicking the cell microenvironments in vivo from either chemical or physical ways. However...

journal_title：Biotechnology and bioengineering

authors： Shen C,Meng Q,Zhang G

更新日期：2013-08-01 00:00:00

abstract：:The sec-alcohol dehydrogenase activity of whole cells of Rhodococcus ruber DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH...

journal_title：Biotechnology and bioengineering

authors： Stampfer W,Kosjek B,Kroutil W,Faber K

更新日期：2003-03-30 00:00:00

abstract：:Metabolic carbon labelling experiments enable a large amount of extracellular fluxes and intracellular carbon isotope enrichments to be measured. Since the relation between the measured quantities and the unknown intracellular metabolic fluxes is given by bilinear balance equations, flux determination from this data s...

journal_title：Biotechnology and bioengineering

authors： Wiechert W,Siefke C,de Graaf AA,Marx A

更新日期：1997-07-05 00:00:00

abstract：:Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-...

journal_title：Biotechnology and bioengineering

authors： Tang Q,Piard C,Lin J,Nan K,Guo T,Caccamese J,Fisher J,Chen Y

更新日期：2018-01-01 00:00:00

abstract：:The goal of this work was to measure and model the effect of thermal exposure on the fluorescence emission of R-phycoerythrin (R-PE). The long-term objective of our work is to assess the feasibility of encapsulating R-PE for use as the critical component of a time-temperature integrator (TTI) for ascertaining the degr...

journal_title：Biotechnology and bioengineering

authors： Vaidya S,Orta-Ramirez A,Smith DM,Ofoli RY

更新日期：2003-08-20 00:00:00

abstract：:The effect of formate and hydrogen on isomerization and syntrophic degradation of butyrate and isobutyrate was investigated using a defined methanogenic culture, consisting of syntrophic isobutyrate-butyrate degrader strain IB, Methanobacterium formicicum strain T1N, and Methanosarcina mazeii strain T18. Formate and h...

journal_title：Biotechnology and bioengineering

authors： Wu WM,Jain MK,Hickey RF,Zeikus JG

更新日期：1996-11-05 00:00:00

abstract：:Although microalgae are considered as a promising feedstock for biofuels, the energy efficiency of the production process needs to be significantly improved. Due to their small size and low concentration in the culture medium, cost-efficient harvesting of microalgae is a major challenge. In this study, the use of elec...

journal_title：Biotechnology and bioengineering

authors： Vandamme D,Pontes SC,Goiris K,Foubert I,Pinoy LJ,Muylaert K

更新日期：2011-10-01 00:00:00

abstract：:In this article, an autonomous four-compartment model that describes the endogenous respiration in an aerobic biodegradation process is proposed and analyzed theoretically. First, the multi-time scale of the system's behavior, to be taken into account in subsequent analyses, is emphasized. Then, an identifiability and...

journal_title：Biotechnology and bioengineering

authors： Keesman KJ,Spanjers H,van Straten G

更新日期：1998-01-20 00:00:00

abstract：:The starting point for the present investigations was the finding that increasing influent concentrations from 10 to 380 mmol/L glucose decreased the attainable growth rate of an acidogenic population in continuous culture from 0.52 to 0.05 h(-1) To account for this phenomenon, a new kinetic model is developed that co...

journal_title：Biotechnology and bioengineering

authors： Van Den Heuvel JC,Beeftink HH

更新日期：1988-05-01 00:00:00

abstract：:A method for purifying alpha-amylase inhibitor from wheat meal based on immobilized metal affinity with a thermosensitive copolymer is developed. The studies represent the thermoprecipitation properties of the copolymers of N-isopropylacrylamide (NIPAM) with iminodiacetic acid (IDA) and 1-vinylimidazole (VI), respecti...

journal_title：Biotechnology and bioengineering

authors： Kumar A,Galaev IY,Mattiasson B

更新日期：1998-09-20 00:00:00

abstract：:We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 +/...

journal_title：Biotechnology and bioengineering

authors： Taira S,Du YZ,Hiratsuka Y,Uyeda TQ,Yumoto N,Kodaka M

更新日期：2008-02-15 00:00:00

abstract：:A recombinant strain of Salmonella enterica serovar Typhimurium (mutant in propionate-activation activity) was metabolically engineered to control the composition of poly(3-hydroxybutyrate-co-3-hydroxy- valerate) (PHBV), a polyhydroxyalkanoate copolymer with commercially desirable properties. A gene (prpE) encoding pr...

journal_title：Biotechnology and bioengineering

authors： Aldor I,Keasling JD

更新日期：2001-09-01 00:00:00

abstract：:To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make...

journal_title：Biotechnology and bioengineering

authors： Oh MK,Kim BG,Park SH

更新日期：1995-09-20 00:00:00

abstract：:The effect of hydrogen and carbon dioxide partial pressure on the growth of the extremely thermophilic archaebacterium Pyrodictium brockii at 98 degrees C was investigated. Previous work with this bacterium has been done using an 80:20 hydrogen-carbon dioxide gas phase with a total pressure of 4 atm; no attempt has be...

journal_title：Biotechnology and bioengineering

authors： Parameswaran AK,Schicho RN,Soisson JP,Kelly RM

更新日期：1988-08-05 00:00:00

abstract：:For the commercial exploitation of lipase biocatalysis to be successful, it is essential that effective supports are selected for lipase immobilization. In this study hydrophobic controlled-pore glasses have been used as model systems for the immobilization of Rhizomucor miehei lipase. The effect of pore diameter and ...

journal_title：Biotechnology and bioengineering

authors： Bosley JA,Clayton JC

更新日期：1994-04-25 00:00:00

abstract：:Acidic Golgi pH plays an important role in protein glycosylation, one of the critical quality attributes of therapeutic proteins. To determine the intracellular Golgi pH during culture, stable Chinese hamster ovary (CHO) cell clones expressing pHluorin2, a ratiometric pH-sensitive fluorescent protein (FP), in the cis-...

journal_title：Biotechnology and bioengineering

authors： Lee JH,Kim J,Park JH,Heo WD,Lee GM

更新日期：2019-05-01 00:00:00

abstract：:Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

journal_title：Biotechnology and bioengineering

authors： Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

更新日期：2006-02-20 00:00:00

abstract：:We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell d...

journal_title：Biotechnology and bioengineering

authors： Echtenkamp PL,Wilson DB,Shuler ML

更新日期：2009-02-15 00:00:00

abstract：:A kinetic model for the agglomeration of milk micelles following kappa-casein hydrolysis is described. The key features of the model are: (1) the surface potential of casein micelles is sufficient to explain the colloidal stability of the milk system; (2) the reduction in surface potential following kappa-casein hydro...

journal_title：Biotechnology and bioengineering

authors： Carlson A,Hill CG Jr,Olson NF

更新日期：1987-04-01 00:00:00

abstract：:Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liv...

journal_title：Biotechnology and bioengineering

authors： Skardal A,Devarasetty M,Forsythe S,Atala A,Soker S

更新日期：2016-09-01 00:00:00

abstract：:An experimental device was constructed to allow nearly simultaneous measurements to be made on temperature and gas composition at different depths in a solid-substrate fermentation bed. The time-dependent values of temperature, mol % O(2) and mol % CO(2) were measured at five positions in beds 6.35 cm (2.5 in.) deep. ...

journal_title：Biotechnology and bioengineering

authors： Rathbun BL,Shuler ML

更新日期：1983-04-01 00:00:00

abstract：:Expanded bed adsorption is a new downstream processing technique forcapture of proteins directly from unclarified feedstocks. Expanded bed adsorption reduces the number of operations in purification processes by combining clarification, concentration, and capture into one operation. It is based on stable fluidization ...

journal_title：Biotechnology and bioengineering

authors： Barnfield Frej AK,Hjorth R,Hammarström A

更新日期：1994-10-01 00:00:00

abstract：:Gene expression microarrays are a relatively new technology, dating back just a few years, yet they have already become a very widely used tool in biology, and have evolved to a wide range of applications well beyond their original design intent. However, while the use of microarrays has expanded, and the issues of pe...

journal_title：Biotechnology and bioengineering

authors： Grant GR,Manduchi E,Pizarro A,Stoeckert CJ Jr

更新日期：2003-12-30 00:00:00

abstract：:The specific molecular interactions of alpha-helical peptide, human glucagon (i.e., intermolecular self-association and specific receptor-binding affinity) provided a rationale for using the glucagon as the fusion expression partner to achieve high productivity of foreign proteins both in vivo (in bacterial fusion-exp...

journal_title：Biotechnology and bioengineering

authors： Kim DY,Lee J,Saraswat V,Park YH

更新日期：2000-08-20 00:00:00

abstract：:An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured...

journal_title：Biotechnology and bioengineering

authors： Xu M,Banc A,Woodruff TK,Shea LD

更新日期：2009-06-01 00:00:00

abstract：:The majority of influenza vaccines are manufactured using embryonated hens' eggs. The potential occurrence of a pandemic outbreak of avian influenza might reduce or even eliminate the supply of eggs, leaving the human population at risk. Also, the egg-based production technology is intrinsically cumbersome and not eas...

journal_title：Biotechnology and bioengineering

authors： George M,Farooq M,Dang T,Cortes B,Liu J,Maranga L

更新日期：2010-08-15 00:00:00

abstract：:The production of beta-galactosidase from the E. coli phoA-lacZ fusion gene was studied to compare the gene expression behavior of two cloning methods: insertion to multicopy plasmids and integration into host cell's chromosome. The chromosome-integrating strain showed more tight control of fusion gene expression leve...

journal_title：Biotechnology and bioengineering

authors： Shin PK,Seo JH

更新日期：1990-12-20 00:00:00

abstract：:A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...

journal_title：Biotechnology and bioengineering

authors： Gerigk M,Bujnicki R,Ganpo-Nkwenkwa E,Bongaerts J,Sprenger G,Takors R

更新日期：2002-12-30 00:00:00

abstract：:Incremental truncation is a method for constructing libraries of every one base pair truncation of a segment of DNA. Incremental truncation libraries can be created using a time-dependent nuclease method or through the incorporation of alpha-phosphothioate dNTPs by PCR or by primer extension (THIO(pcr) truncation and ...

journal_title：Biotechnology and bioengineering

authors： Ostermeier M

更新日期：2003-06-05 00:00:00