The inhibition of Escherichia coli lac operon gene expression by antigene oligonucleotides-mathematical modeling.

abstract：:10-Deacetylabaccatin III (10 DAB), an important precursor for paclitaxel semisynthesis, is enhanced in yew extracts using C10-deacetylase and C13-deacylase enzymes.(4) C10-deacetylase is an intracellular enzyme produced by the fermentation of a soil microorganism, Nocardioides luteus (SC 13912). During the fermentatio...

journal_title：Biotechnology and bioengineering

authors： Nanduri VB,Hanson RL,Laporte TL,Ko RY,Patel RN,Szarka LJ

更新日期：1995-12-05 00:00:00

abstract：:Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human superoxide dismutase. Induction with IPTG lead to an increase in protein content and cell size and a termination of cell div...

journal_title：Biotechnology and bioengineering

authors： Soriano E,Borth N,Katinger H,Mattanovich D

更新日期：2002-10-05 00:00:00

abstract：:Uranium competitive uptake experiments by Rhizopus arrhizus were carried out at three different solution pH levels and in the presence of different concentrations of competing aluminum ions in order to examine the competing ion effect. The coion effect became more pronounced as the coion concentration in solution and ...

journal_title：Biotechnology and bioengineering

authors： Tsezos M,Georgousis Z,Remoudaki E

更新日期：1997-07-05 00:00:00

abstract：:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...

journal_title：Biotechnology and bioengineering

authors： Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

更新日期：2008-08-01 00:00:00

abstract：:Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

journal_title：Biotechnology and bioengineering

authors： Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

更新日期：2012-01-01 00:00:00

abstract：:A rapid method is developed to analyze the kinetics of thermal inactivation of enzymes that exhibit a nonlinear biphasic log(activity)-time relationship. Thermal destruction experiments on alcohol dehydrogenase from baker's yeast demonstrate the applicability of the method. The method is based on physical consideratio...

journal_title：Biotechnology and bioengineering

authors： Nath S

更新日期：1996-01-05 00:00:00

abstract：:Numerous biotechnological production processes are based on the submerse cultivation of filamentous fungi. Process design, however, is often hampered by the complex growth pattern of these organisms. In the morphologic development of coagulating filamentous fungi, like Aspergillus niger, conidial aggregation is the fi...

journal_title：Biotechnology and bioengineering

authors： Lin PJ,Grimm LH,Wulkow M,Hempel DC,Krull R

更新日期：2008-02-01 00:00:00

abstract：:A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...

journal_title：Biotechnology and bioengineering

authors： Niranjan SC,San KY

更新日期：1989-08-05 00:00:00

abstract：:Generating stable, high-producing cell lines for recombinant protein production requires an understanding of the potential limitations in the cellular machinery for protein expression. In order to increase our understanding of what makes a stable high producer, we have generated a panel of 17 recombinant monoclonal an...

journal_title：Biotechnology and bioengineering

authors： Chusainow J,Yang YS,Yeo JH,Toh PC,Asvadi P,Wong NS,Yap MG

更新日期：2009-03-01 00:00:00

abstract：:Buddleja davidii is a unique biomass that has many attractive agroenergy features, especially its wide range of growth habitat. The anatomical characteristics of B. davidii were investigated before and after ethanol organosolv pretreatment (one of the leading pretreatment technologies) in order to further understand t...

journal_title：Biotechnology and bioengineering

authors： Hallac BB,Ray M,Murphy RJ,Ragauskas AJ

更新日期：2010-12-01 00:00:00

abstract：:Two shock loads of a commercial detergent (I-150 mg chemical oxygen demand (COD)/L, fed for 56 h; II-300 mg COD/L fed for 222 h) were applied in a lab-scale Expanded Granular Sludge Blanket (EGSB) reactor, fed with 1,500 mg COD/L of ethanol. The impact of the surfactant was assessed in terms of granular sludge morphol...

journal_title：Biotechnology and bioengineering

authors： Costa JC,Abreu AA,Ferreira EC,Alves MM

更新日期：2007-09-01 00:00:00

abstract：:Saccharomyces cerevisiae is a widely used host organism for the production of heterologous proteins, often cultivated in glucose-based fed-batch processes. This production system however has many factors limiting the productivity, mainly towards the end of the fermentation. For the optimised production of a Camelid an...

journal_title：Biotechnology and bioengineering

authors： van de Laar T,Visser C,Holster M,López CG,Kreuning D,Sierkstra L,Lindner N,Verrips T

更新日期：2007-02-15 00:00:00

abstract：:A starvation-based dissolved oxygen (DO) transient controller was developed to supply growth-limiting substrate to high cell density fed-batch cultures of recombinant Escherichia coli. The algorithm adjusted a preexisting feed rate in proportion to the culture's oxygen demand, which was estimated from transients in th...

journal_title：Biotechnology and bioengineering

authors： Whiffin VS,Cooney MJ,Cord-Ruwisch R

更新日期：2004-02-20 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...

journal_title：Biotechnology and bioengineering

authors： Zhang HF,Ma L,Wang ZH,Chen GQ

更新日期：2009-10-15 00:00:00

abstract：:A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...

journal_title：Biotechnology and bioengineering

authors： Sadana A,Henley JP

更新日期：1987-10-20 00:00:00

abstract：:The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...

journal_title：Biotechnology and bioengineering

authors： Stoodley P,Yang S,Lappin-Scott H,Lewandowski Z

更新日期：1997-12-20 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:The cell cycle dependency of foreign gene expression in recombinant mouse L cells was investigated. Two different recombinant mouse L cell lines having the glucocorticoid receptor-encoding gene and the lacZ reporter gene were used in this study. The lacZ gene expression was controlled by the glucocorticoid-inducible m...

journal_title：Biotechnology and bioengineering

authors： Gu MB,Todd P,Kompala DS

更新日期：1996-05-05 00:00:00

abstract：:Isothermal microcalorimeters (IMC) are highly sensitive instruments that allow the measurement of heat flow in the microwatt range. Due to their detection of minute thermal heat, IMC techniques have been used in numerous biological applications, including the study of fermentation processes, pharmaceutical development...

journal_title：Biotechnology and bioengineering

authors： Santoro R,Braissant O,Müller B,Wirz D,Daniels AU,Martin I,Wendt D

更新日期：2011-12-01 00:00:00

abstract：:We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...

journal_title：Biotechnology and bioengineering

authors： Fu J,VanDusen WJ,Kolodin DG,O'Keefe DO,Herber WK,George HA

更新日期：1996-03-05 00:00:00

abstract：:Uranium contamination is a serious concern at several sites motivating the development of novel treatment strategies such as the Geobacter-mediated reductive immobilization of uranium. However, this bioremediation strategy has not yet been optimized for the sustained uranium removal. While several reactive-transport m...

journal_title：Biotechnology and bioengineering

authors： Zhao J,Scheibe TD,Mahadevan R

更新日期：2011-07-01 00:00:00

abstract：:Lentiviral vectors (LVs) enveloped with an engineered Sindbis virus glycoprotein can specifically bind to dendritic cells (DCs) through the surface receptor DC-SIGN and induce antigen expression, thus providing an efficient method for delivering DC-directed vaccines. In this study, we constructed a stable producer lin...

journal_title：Biotechnology and bioengineering

authors： Lee CL,Chou M,Dai B,Xiao L,Wang P

更新日期：2012-06-01 00:00:00

abstract：:An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Koshy G,Ramseier M

更新日期：1989-01-05 00:00:00

abstract：:An innovative type of biofilm model is derived by combining an individual description of microbial particles with a continuum representation of the biofilm matrix. This hybrid model retains the advantages of each approach, while providing a more realistic description of the temporal development of biofilm structure in...

journal_title：Biotechnology and bioengineering

authors： Alpkvist E,Picioreanu C,van Loosdrecht MC,Heyden A

更新日期：2006-08-05 00:00:00

abstract：:A novel method has been developed for the separation of bioproducts from yeast cells. The method uses a combination of physical, chemical, and biological agents such as lytic enzymes, osmotic supports, and spheroplast stabilizers. Using this technique, products (proteins and enzymes) can be released from specific cell...

journal_title：Biotechnology and bioengineering

authors： Huang RB,Andrews BA,Asenjo JA

更新日期：1991-11-01 00:00:00

abstract：:The biological molecule responsible for the suppression of pyritic sulfur in fine coal simulated froth flotation treated with bacteria was identified. Protein was found to be the most effective agent in pyrite suppression of the three cell components (protein, lipid, and carbohydrate) assayed. Coal recovery and ash re...

journal_title：Biotechnology and bioengineering

authors： Stainthorpe AC

更新日期：1989-02-05 00:00:00

abstract：:Trichoderma reesei (QM 9414) produced cellulase in continuous culture, on media containing xylose (1%) supplemented with sorbose (0.3%) to induce cellulase production. Maximum cell mass of 4.54 kg/m(3) occurred at pH 4.0 and a dilution rate of 0.0391 h(-1) where residual substrate was 0.43 kg/m(3), but no cellulase wa...

journal_title：Biotechnology and bioengineering

authors： Schafner DW,Toledo RT

更新日期：1992-04-05 00:00:00

abstract：:Synthetic mixtures of predominant lignocellulosic hexose sugars were supplemented with separate aliquots of three inhibitory compounds (furfural, hydroxymethylfurfural (HMF), and acetic acid) in a series of concentrations and fermented by the spent sulfite liquor (SSL)-adapted yeast strain Tembec T1 and the natural is...

journal_title：Biotechnology and bioengineering

authors： Keating JD,Panganiban C,Mansfield SD

更新日期：2006-04-20 00:00:00

abstract：:Mucopolysaccharidosis (MPS) Type II is caused by mutations in the gene encoding the lysosomal enzyme, iduronate 2-sulfatase (IDS). The majority of MPSII cases affect the brain. However, enzyme replacement therapy with recombinant IDS does not treat the brain, because IDS is a large molecule drug that does not cross th...

journal_title：Biotechnology and bioengineering

authors： Boado RJ,Ka-Wai Hui E,Zhiqiang Lu J,Pardridge WM

更新日期：2014-11-01 00:00:00

abstract：:In this report, alpha-Amylase originating from Bacillus subtilis (liquefying type) was immobilized on partially imidoesterized polyacrylonitrile (PAN) by covalent bonding. For the preparation of immobilized alpha-amylase, which has a high activity and high stability to repeated use, the optimum conditions for the prep...

journal_title：Biotechnology and bioengineering

authors： Handa T,Hirose A,Akino T,Watanabe K,Tsuchiya H

更新日期：1983-12-01 00:00:00