Abstract:
:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of axial mixing of fluid from the deoxygenated end chamber. The bioreactor was used to supply air to a hybridoma mammalian cell line, and the calculated oxygen uptake showed that high-density cultures could be maintained in a 20 mL, single-dimpled cultures could be maintained in a 20 mL, single-dimpled membrane system. Indirect aeration of a 2 L continuous stirred tank reactor, by a double-membrane system, showed that air could be supplied to mammalian cells at cell densities of approximately 4 x 10(6) /mL.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJdoi
10.1002/bit.260381016subject
Has Abstractpub_date
1991-12-05 00:00:00pages
1233-8issue
10eissn
0006-3592issn
1097-0290journal_volume
38pub_type
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