Uptake of cadmium and zinc by the alga Chlorella vulgaris: II. Multi-ion situation.

abstract：:The cometabolic transformation of 2,4,6-trinitrotoluene (TNT) by an immobilized Phanerochaete chrysosporium culture was investigated under different TNT and/or glycerol feeding conditions in a 5-L reactor. In the fed-batch feeding mode, as a result of four spiking events at an average feeding rate of 20 mg TNT L(-1) d...

journal_title：Biotechnology and bioengineering

authors： Rho D,Hodgson J,Thiboutot S,Ampleman G,Hawari J

更新日期：2001-05-20 00:00:00

abstract：:Efficient cofactor regeneration and reuse are highly desired for many important biotransformation applications. Here we show for the first time that cofactor NAD(H) covalently attached to micro particles, which can be easily recovered and reused, effectively mediated multistep reactions catalyzed by enzymes that were ...

journal_title：Biotechnology and bioengineering

authors： El-Zahab B,Donnelly D,Wang P

更新日期：2008-02-15 00:00:00

abstract：:The stability of immobilized preparations of xanthine oxidase and urate oxidase was studied, and optimized, because of the potential joint use of both enzymes in clinical analysis. Xanthine oxidase was immobilized on cellulose, Sepharose, hornblende, Enzacryl-TIO, and porous glass. Thehalf-lives of these preparations ...

journal_title：Biotechnology and bioengineering

authors： Johnson DB,Coughlan MP

更新日期：1978-07-01 00:00:00

abstract：:The first genome scale model (GSM) for Streptomyces leeuwenhoekii C34 was developed to study the biosynthesis pathways of specialized metabolites and to find metabolic engineering targets for enhancing their production. The model, iVR1007, consists of 1,722 reactions, 1,463 metabolites, and 1,007 genes, it includes th...

journal_title：Biotechnology and bioengineering

authors： Razmilic V,Castro JF,Andrews B,Asenjo JA

更新日期：2018-07-01 00:00:00

abstract：:Phage Φ80 can infect Escherichia coli in a stealthy manner and persist by forming lysogens. Such Φ80 lysogens are fairly common and often go undetected unless the host is grown at temperatures below 37°C. Since low growth temperatures are required for growing temperature-sensitive mutants and often preferred for large...

journal_title：Biotechnology and bioengineering

authors： Srinivas S,Cronan JE

更新日期：2019-07-01 00:00:00

abstract：:Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening and to provide useful information about various cell-chemical interactions. A computer aided an...

journal_title：Biotechnology and bioengineering

authors： Li XM,Liang BS,Wang HY

更新日期：1988-02-20 00:00:00

abstract：:In our preceding article, we demonstrated a procedure based upon enzymic flow microcalorimetry using an enzyme thermistor (ET) to characterize the microkinetic properties of an immobilized enzyme (IME) and its further application in the screening of IMEs. To consider the ET method (single ET unit, ET system 1) as stan...

journal_title：Biotechnology and bioengineering

authors： Gemeiner P,Docolomansky P,Nahálka J,Stefuca V,Danielsson B

更新日期：1996-01-05 00:00:00

abstract：:The activities of lipase from Candida cylindracea and Rhizopus delemar have been investigated in water/AOT/iso-octane reverse micellar media through the use of two esterification reactions: fatty acid-alcohol esterification and glyceride synthesis. Such media promotes the occurrence of these two lipase-catalyzed react...

journal_title：Biotechnology and bioengineering

authors： Hayes DG,Gulari E

更新日期：1990-04-05 00:00:00

abstract：:Affinity chromatography using immobilized S-protein was used for the screening of affinity peptide ligands from two soluble peptide libraries. Peptide library I consisted of octamers with glycine (G) at both termini of each peptide, i.e. GXXXXXXG. The six center positions were constructed using random sequences of six...

journal_title：Biotechnology and bioengineering

authors： Huang PY,Carbonell RG

更新日期：1999-06-20 00:00:00

abstract：:Recombinant Escherichia coli harboring the medium-chain-length (MCL) polyhydroxyalkanoate (PHA) synthase gene has been shown to accumulate MCL-PHAs from fatty acids when FadB is inactive. However, the enzymes in fadB mutant E. coli responsible for channeling the beta-oxidation intermediates to PHA biosynthesis have no...

journal_title：Biotechnology and bioengineering

authors： Park SJ,Yup Lee S

更新日期：2004-06-20 00:00:00

abstract：:An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Koshy G,Ramseier M

更新日期：1989-01-05 00:00:00

abstract：:The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentrat...

journal_title：Biotechnology and bioengineering

authors： López JC,Porca E,Collins G,Pérez R,Rodríguez-Alija A,Muñoz R,Quijano G

更新日期：2017-03-01 00:00:00

abstract：:Nitrifying bacteria, cyanobacteria, and algae are important microorganisms in open pond wastewater treatment systems. Nitrification involving the sequential oxidation of ammonia to nitrite and nitrate, mainly due to autotrophic nitrifying bacteria, is essential to biological nitrogen removal in wastewater and global n...

journal_title：Biotechnology and bioengineering

authors： Choi O,Das A,Yu CP,Hu Z

更新日期：2010-12-15 00:00:00

abstract：:For clinical trials, large amounts of high-titer retroviral supernatants are required. However, retroviral concentration is relatively low compared with other viral vectors. Moreover, less than half of retroviral vectors suspended in a collected supernatant are infectious because of their short half-lives. In this stu...

journal_title：Biotechnology and bioengineering

authors： Kwon YJ,Peng CA

更新日期：2005-05-05 00:00:00

abstract：:Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

journal_title：Biotechnology and bioengineering

authors： Siano SA

更新日期：1996-12-20 00:00:00

abstract：:In order to evaluate the sensitivity of hepatocellular cultures to variations in both substrate stiffness and bioactive ligand presentation, hepatocytes were cultured on differentially compliant polyacrylamide gel discs functionalized with varying amounts of the ECM ligand, fibronectin (FN). Subconfluent cell cultures...

journal_title：Biotechnology and bioengineering

authors： Semler EJ,Lancin PA,Dasgupta A,Moghe PV

更新日期：2005-02-05 00:00:00

abstract：:Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell sorting (FACS). For comparison, cell pools and cell lines were al...

journal_title：Biotechnology and bioengineering

authors： Oberbek A,Matasci M,Hacker DL,Wurm FM

更新日期：2011-03-01 00:00:00

abstract：:Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig...

journal_title：Biotechnology and bioengineering

authors： Woodley JM,Brazier AJ,Lilly MD

更新日期：1991-01-20 00:00:00

abstract：:We describe a novel procedure to estimate the net growth rate of biofilms on multiple substrates. The approach is based on diffusion-reaction mass balances for chemical species in a continuum biofilm model with reaction kinetics corresponding to a Double-Monod expression. This analytical model considers a heterogeneou...

journal_title：Biotechnology and bioengineering

authors： Gonzo EE,Wuertz S,Rajal VB

更新日期：2014-11-01 00:00:00

abstract：:The enzymatic synthesis of polyesters from activated diesters and diols has been investigated. Differences between enzymatic synthesis and traditional chemical condensation processes are discussed. The disappearance of monomers during the initial phase of reaction indicates that enzyme has a higher specificity for tra...

journal_title：Biotechnology and bioengineering

authors： Chaudhary AK,Beckman EJ,Russell AJ

更新日期：1997-07-05 00:00:00

abstract：:Anerobic biofilms with dominantly acidogenic bacteria were grown in fixed-bed recycle reactors. The influence of calcium concentration in the culture medium on biofilm mass accumulation, immobilized calcium concentration, and biofilm-specific activity was investigated. The results indicate that the biofilm mass accumu...

journal_title：Biotechnology and bioengineering

authors： Huang J,Pinder KL

更新日期：1995-02-05 00:00:00

abstract：:Hydrogen production by Rhodobacter capsulatus is an anaerobic, photobiological process requiring specific mixing conditions. In this study, an innovative design of a photobioreactor is proposed. The design is based on a plate-type photobioreactor with an interconnected meandering channel to allow culture mixing and H2...

journal_title：Biotechnology and bioengineering

authors： Turon V,Ollivier S,Cwicklinski G,Willison JC,Anxionnaz-Minvielle Z

更新日期：2020-12-15 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) plays a fundamental role in the fungal ligninolytic secretome, acting as a supplier of H2 O2 . Despite its highly selective mechanism of action, the presence of this flavooxidase in different biotechnological settings has hitherto been hampered by the lack of appropriate heterologous express...

journal_title：Biotechnology and bioengineering

authors： Viña-Gonzalez J,Elbl K,Ponte X,Valero F,Alcalde M

更新日期：2018-07-01 00:00:00

abstract：:A method was developed for obtaining high signal-to-noise 13C NMR spectra of intracellular compounds in metabolically active cultured cells. The method allows TCA cycle labeling kinetics to be determined in real time without significant oxygen transport limitations. Cells were immobilized on the surface of nonporous m...

journal_title：Biotechnology and bioengineering

authors： Mancuso A,Beardsley NJ,Wehrli S,Pickup S,Matschinsky FM,Glickson JD

更新日期：2004-09-30 00:00:00

abstract：:Ultrafiltration through Carbosep M(4) mineral membrane of protein solutions of decreasing complexity (whey before and after centrifugation or clarification, beta-lactoglobulin) was studied. Mathematical models were used to explain variations in flux with time. Taking into account variations in protein retention and hy...

journal_title：Biotechnology and bioengineering

authors： Taddei C,Daufin G,Aimar P,Sanchez V

更新日期：1989-06-20 00:00:00

abstract：:The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain...

journal_title：Biotechnology and bioengineering

authors： Kunert R,Steinfellner W,Purtscher M,Assadian A,Katinger H

更新日期：2000-01-05 00:00:00

abstract：:Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liv...

journal_title：Biotechnology and bioengineering

authors： Skardal A,Devarasetty M,Forsythe S,Atala A,Soker S

更新日期：2016-09-01 00:00:00

abstract：:Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On th...

journal_title：Biotechnology and bioengineering

authors： Meyer F,Gehmlich I,Guthke R,Górak A,Knorre WA

更新日期：1998-07-20 00:00:00

abstract：:Several researchers have demonstrated that the presence of a heterologous protein in recombinant Escherichia coli elicits a response similar to the heat-shock response, which includes enhanced protease expression. The present work detects, quantifies, and characterizes intracellular protease activity in E. coli that a...

journal_title：Biotechnology and bioengineering

authors： Harcum SW,Bentley WE

更新日期：1993-09-05 00:00:00

abstract：:The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion...

journal_title：Biotechnology and bioengineering

authors： Love KR,Panagiotou V,Jiang B,Stadheim TA,Love JC

更新日期：2010-06-01 00:00:00