Abstract:
:This communication demonstrates that two-phase aqueous mixed (nonionic/ionic) micellar systems have the potential for improving the separation of proteins from viruses. Specifically, two separation experiments were performed to show that the addition of the anionic surfactant sodium dodecyl sulfate (SDS) to the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C(10)E(4)) micellar system increases the yield of a model net positively charged protein, lysozyme, in the micelle-rich phase from 75 to 95%, while still maintaining approximately the same yield of a model net negatively charged virus, bacteriophage P22, in the micelle-poor phase (97% vs. 98%).
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Kamei DT,King JA,Wang DI,Blankschtein Ddoi
10.1002/bit.10377subject
Has Abstractpub_date
2002-10-20 00:00:00pages
233-6issue
2eissn
0006-3592issn
1097-0290journal_volume
80pub_type
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