Abstract:
:In the first part of our work, we combined compositional analysis, pretreatment and enzyme hydrolysis for fractionated pith, rind, and leaf tissues from a hybrid stay-green corn, in order to identify the role of structural characteristics on enzyme hydrolysis of cell walls. Hydrolysis experiments coupled with chemical analysis of the different fractions of corn stover showed significant differences in cell wall structure before and after liquid hot water pretreatment. The extent of enzyme hydrolysis followed the sequence rind < leaves < pith with 90% conversion of cellulose to glucose in 24 h in the best cases. Since similar lignin contents remained after liquid hot water pretreatment of leaves, rind, and pith, our results indicated that the amount of lignin alone is not sufficient to explain the different enzymatic hydrolysis characteristics of the fractions. While the role of structural characteristics on enzyme hydrolysis of cell walls is measured as described in part I, the SEM images presented in this part II of our work show that sugar yields from enzymatic hydrolysis of corn fractions correlate with changes in plant cell wall structure both before and after liquid hot water pretreatment.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Zeng M,Ximenes E,Ladisch MR,Mosier NS,Vermerris W,Huang CP,Sherman DMdoi
10.1002/bit.23335subject
Has Abstractpub_date
2012-02-01 00:00:00pages
398-404issue
2eissn
0006-3592issn
1097-0290journal_volume
109pub_type
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