Abstract:
:The potential of bacteriophage lambda as an expression vector for a large scale production of cloned-gene proteins was evaluated in batch and continuous bioreactors using a temperature-sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or lytic state. A temperature switch from 32 degrees C (or below) to 38 degrees C (or above) forces the phage to go from the lysogenic state to the lytic state. Temperature cycling and a two-reactor system were used for continuous cultures. For the latter the first reactor is maintained in the lysogenic state at a lower temperature to stably maintain the foreign DNA in the host cell, while the second reactor is maintained in the lytic state to force replication of the cloned-gene and overproduction of its products. The results are promising but suggest a greater potential for a mutant which lacks the Q gene which is responsible for host cell lysis and packaging of phage particles.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Park TH,Seo JH,Lim HCdoi
10.1002/bit.260370402subject
Has Abstractpub_date
1991-02-20 00:00:00pages
297-302issue
4eissn
0006-3592issn
1097-0290journal_volume
37pub_type
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