Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae.

abstract：:This paper presents an experimental analysis of matrix bead size distribution and voidage variations with axial height in an expanded bed adsorption system. Use of a specially constructed expanded bed with side ports has enabled sampling from within the expanded bed along the vertical axis. Particles removed from with...

journal_title：Biotechnology and bioengineering

authors： Willoughby NA,Hjorth R,Titchener-Hooker NJ

更新日期：2000-09-20 00:00:00

abstract：:Lipase (EC 3.1.1.3., from Pseudomonas sp.) was entrapped in collagen membrane containing liquid crystal (4-methoxybenzilidene-4'-n-butylaniline). The activity of the lipase-liquid crystal membrane at an applied voltage of 4 V was 3.4 compared to a membrane tested without imposition of an external electric field. A lin...

journal_title：Biotechnology and bioengineering

authors： Karube I,Yugeta Y,Suzuki S

更新日期：1977-10-01 00:00:00

abstract：:Non-destructive label-free continuous monitoring of in vitro tissue culture is an unmet demand in tissue engineering. Noting that different compositions of cartilage lead to different electrical tissue properties, we propose a new method to measure the electrical conductivity and its anisotropy during in vitro chondro...

journal_title：Biotechnology and bioengineering

authors： Oh TI,Kim C,Karki B,Son Y,Lee E,Woo EJ

更新日期：2015-02-01 00:00:00

abstract：:Several experimental data on continuous cultures of hybridoma cells show that monoclonal antibody productivity is a decreasing function of dilution rate. It has been suggested that this unusual behavior may be due to the arrest of a fraction of cycling cells at a critical point of Phase G(1). Although this hypothesis ...

journal_title：Biotechnology and bioengineering

authors： Cazzador L,Mariani L

更新日期：1993-12-01 00:00:00

abstract：:Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

journal_title：Biotechnology and bioengineering

authors： Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

更新日期：2008-01-01 00:00:00

abstract：:Remarkable increases in enzyme catalytic stability resulting from addition of charged water-soluble polymers have recently been reported, suggesting that use of these polymers may be an attractive general strategy for enzyme stabilization. To test the proposed hypothesis that coulombic forces between water-soluble pol...

journal_title：Biotechnology and bioengineering

authors： Foreman TM,Khalil M,Meier P,Brainard JR,Vanderberg LA,Sauer NN

更新日期：2001-11-01 00:00:00

abstract：:Enzyme engineering is a powerful tool to fine-tune the enzymes. It is a technique by which the stability, activity, and specificity of the enzymes can be altered. The characteristic properties of an enzyme can be amended by immobilization and protein engineering. Among them, protein engineering is the most promising, ...

journal_title：Biotechnology and bioengineering

authors： Ali M,Ishqi HM,Husain Q

更新日期：2020-06-01 00:00:00

abstract：:Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved...

journal_title：Biotechnology and bioengineering

authors： Yoshimoto N,Ikeda Y,Tatematsu K,Iijima M,Nakai T,Okajima T,Tanizawa K,Kuroda S

更新日期：2016-08-01 00:00:00

abstract：:Herein, we report the development of a microbial bioprocess for high-level production of 5-aminolevulinic acid (5-ALA), a valuable non-proteinogenic amino acid with multiple applications in medical, agricultural, and food industries, using Escherichia coli as a cell factory. We first implemented the Shemin (i.e., C4) ...

journal_title：Biotechnology and bioengineering

authors： Miscevic D,Mao JY,Kefale T,Abedi D,Moo-Young M,Perry Chou C

更新日期：2021-01-01 00:00:00

abstract：:The effect of hydrogen and carbon dioxide partial pressure on the growth of the extremely thermophilic archaebacterium Pyrodictium brockii at 98 degrees C was investigated. Previous work with this bacterium has been done using an 80:20 hydrogen-carbon dioxide gas phase with a total pressure of 4 atm; no attempt has be...

journal_title：Biotechnology and bioengineering

authors： Parameswaran AK,Schicho RN,Soisson JP,Kelly RM

更新日期：1988-08-05 00:00:00

abstract：:The diffusion characteristics of sucrose, a nutrient, and yohimbine, a secondary metabolite, in alginate gel beads, with or without entrapped periwinkle (Catharanthus roseus) or apple (Malus domestica) cells, were investigated. Effective diffusivities of both solutes in the gel beads were determined by two different m...

journal_title：Biotechnology and bioengineering

authors： Pu HT,Yang RY

更新日期：1988-09-20 00:00:00

abstract：:The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of...

journal_title：Biotechnology and bioengineering

authors： Dols-Lafargue M,Willemot RM,Monsan PF,Remaud-Simeon M

更新日期：2001-11-05 00:00:00

abstract：:Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor ...

journal_title：Biotechnology and bioengineering

authors： Birnbaum S,Bailey JE

更新日期：1991-04-05 00:00:00

abstract：:Two metabolic engineering tools, namely gene inactivation and gene overexpression, were employed to examine the effects of two genetic modifications on the fermentation characteristics of Clostridium acetobutylicum. Inactivation of the butyrate kinase gene (buk) was examined using strain PJC4BK, while the combined eff...

journal_title：Biotechnology and bioengineering

authors： Harris LM,Desai RP,Welker NE,Papoutsakis ET

更新日期：2000-01-05 00:00:00

abstract：:The microbial degradation of quinoline by Comamonas acidovorans was studied in a laboratory scale stirred tank reactor. In continuous culture experiments using quinoline as a sole source of carbon and nitrogen, it was shown by means of mass balances that quinoline was converted completely to biomass, carbon dioxide, a...

journal_title：Biotechnology and bioengineering

authors： Miethling R,Hecht V,Deckwer WD

更新日期：1993-08-20 00:00:00

abstract：:Cartilage implants for potential in vivo use for joint repair or reconstructive surgery can be created in vitro by growing chondrocytes on biodegradable polymer scaffolds. Implants 1 cm in diameter by 0.176 cm thick were made using isolated calf chondrocytes and polyglucolic acid (PGA). By 6 weeks, the total amount of...

journal_title：Biotechnology and bioengineering

authors： Freed LE,Marquis JC,Langer R,Vunjak-Novakovic G,Emmanual J

更新日期：1994-03-25 00:00:00

abstract：:Alginate microparticles produced by emulsification/internal gelation were investigated as a promising carrier for insulin delivery. The procedure involves the dispersion of alginate solution containing insulin protein, into a water immiscible phase. Gelation is triggered in situ by instantaneous release of ionic calci...

journal_title：Biotechnology and bioengineering

authors： Reis CP,Ribeiro AJ,Neufeld RJ,Veiga F

更新日期：2007-04-01 00:00:00

abstract：:Reducing viscosities of concentrated solutions of therapeutic proteins is important for their subcutaneous and intravenous delivery. Although inorganic salts and optimizing the pH were previously reported to dramatically lower the viscosity of a monoclonal antibody solution, herein we have determined these effects not...

journal_title：Biotechnology and bioengineering

authors： Du W,Klibanov AM

更新日期：2011-03-01 00:00:00

abstract：:A mathematical model has been employed to examine the interplay of reaction and mass transfer in immobilized enzyme systems involving reaction-generated enzyme poisions. Deactivation rates can be significantly reduced in some cases by catalyzing a purification reaction in which the poison is transformed into an innocu...

journal_title：Biotechnology and bioengineering

authors： Yeung SY,Cho Y,Bailey JE

更新日期：1978-08-01 00:00:00

abstract：:The mixed-culture anaerobic conversion of lactose to organic acids in a bench-scale continuous-flow stirredtank fermentor is considered. The major acidogenic end-product distribution with respect to the dilution rate are presented. A Monod chemostat model is employed to describe a microbial growth, and the influence o...

journal_title：Biotechnology and bioengineering

authors： Kissalita WS,Lo KV,Pinder KL

更新日期：1989-01-25 00:00:00

abstract：:Pseudomonads are serious candidates for siderophore production applied to toxic metal (TM) solubilization. The bioaugmentation of contaminated soils by these TM-solubilizing bacteria combined with phytoextraction is an emerging clean-up technology. Unfortunately, siderophore synthesis may be drastically reduced by sol...

journal_title：Biotechnology and bioengineering

authors： Braud A,Jézéquel K,Léger MA,Lebeau T

更新日期：2006-08-20 00:00:00

abstract：:In this article, a kinetic model is developed and presented for biological nutrient removal (BNR) activated sludge (BNRAS) systems in general, but for external nitrification (EN) BNRAS (ENBNRAS) systems in particular. The model is based on the UCTPHO model, but includes some significant modifications, such as anoxic P...

journal_title：Biotechnology and bioengineering

authors： Hu ZR,Wentzel MC,Ekama GA

更新日期：2007-12-15 00:00:00

abstract：:The effects of growing Clostridium acetobutylicum NCIB 8052 in a chemostat under conditions of glucose, NH(4) (+), PO(4) (3-), Mg(2+), and Fe(2+) limitation were examined. It was noted that limitation of any major nutrient resulted in the same fermentation pattern. Conditions where minor nutrient levels were reduced a...

journal_title：Biotechnology and bioengineering

authors： McNeil B,Kristiansen B

更新日期：1987-02-01 00:00:00

abstract：:Regioselectivity of glycosyltransferases offers an important means to overcome the limitations of chemical synthesis of small molecule glycosides. In this study we explore a large multigene family of UDP-glucose:glycosyltransferases of Arabidopsis for their potential as novel biocatalysts for in vitro synthesis and wh...

journal_title：Biotechnology and bioengineering

authors： Lim EK,Ashford DA,Hou B,Jackson RG,Bowles DJ

更新日期：2004-09-05 00:00:00

abstract：:An immobilized enzyme (pancreatic ribonuclease bound to porous titania) was investigated for the degradation of purified yeast ribonucleic acid as a substrate. The immobilized enzyme is active and stable in the pH range 4--8. Dependence of enzymatic activity on ionic strength, pH, temperature, fluid flow rate, and sub...

journal_title：Biotechnology and bioengineering

authors： Dale BE,White DH

更新日期：1979-09-01 00:00:00

abstract：:Overexpression of the cyclin-dependent kinase inhibitor p27 and exposure to low temperature (30 degrees C) represent two strategies to establish controlled proliferation processes for production of therapeutic proteins using Chinese hamster ovary (CHO) cells. Here we analyze the effect of growth inhibition on the qual...

journal_title：Biotechnology and bioengineering

authors： Kaufmann H,Mazur X,Marone R,Bailey JE,Fussenegger M

更新日期：2001-03-20 00:00:00

abstract：:Cell culture longevity in fed-batch culture of hybridomas is often limited by elevated medium osmolality caused by repeated nutrient feeding. Shotwise feeding of 10x Dulbecco's modified Eagle's medium (DMEM) concentrates elevated the osmolality of medium up to 540 mOsm/kg at the end of fed-batch culture of S3H5/gamma2...

journal_title：Biotechnology and bioengineering

authors： Ryu JS,Lee GM

更新日期：1999-01-05 00:00:00

abstract：:Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlacto...

journal_title：Biotechnology and bioengineering

authors： Xie S,Svec F,Fréchet JM

更新日期：1999-01-05 00:00:00

abstract：:The partitioning behavior of viruses in the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C10E4) micellar system cannot be fully explained by considering solely the repulsive, steric, excluded-volume interactions that operate between the viruses and the nonionic C10E4 micelles. Specifically, an excluded-vo...

journal_title：Biotechnology and bioengineering

authors： Kamei DT,Liu CL,Haase-Pettingell C,King JA,Wang DI,Blankschtein D

更新日期：2002-04-20 00:00:00

abstract：:Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...

journal_title：Biotechnology and bioengineering

authors： Bryers JD

更新日期：1985-05-01 00:00:00