A general kinetic model for biological nutrient removal activated sludge systems: model development.

abstract：:Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined i...

journal_title：Biotechnology and bioengineering

authors： Kimura M,Michizoe J,Oakazaki SY,Furusaki S,Goto M,Tanaka H,Wariishi H

更新日期：2004-11-20 00:00:00

abstract：:The heterotrophic marine alga Crypthecodinium cohnii is known to produce docosahexaenoic acid (DHA), a polyunsaturated fatty acid with food and pharmaceutical applications, during batch cultivation on complex media containing sea salt, yeast extract, and glucose. In the present study, fed-batch cultivation was studied...

journal_title：Biotechnology and bioengineering

authors： De Swaaf ME,Sijtsma L,Pronk JT

更新日期：2003-03-20 00:00:00

abstract：:The treatment of large segmental bone defects remains a challenge as infection, delayed union, and nonunion are common postoperative complications. A three-dimensional printed bioresorbable and physiologically load-sustaining graft substitute was developed to mimic native bone tissue for segmental bone repair. Fabrica...

journal_title：Biotechnology and bioengineering

authors： Chung R,Kalyon DM,Yu X,Valdevit A

更新日期：2018-09-01 00:00:00

abstract：:Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO2 . To quantify and understand how these process parameters affect yeast physiology, this study investigates in...

journal_title：Biotechnology and bioengineering

authors： Hakkaart X,Liu Y,Hulst M,El Masoudi A,Peuscher E,Pronk J,van Gulik W,Daran-Lapujade P

更新日期：2020-03-01 00:00:00

abstract：:The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

journal_title：Biotechnology and bioengineering

authors： Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

更新日期：2006-08-20 00:00:00

abstract：:The increasing accessibility of mass isotopomer data via GC-MS and NMR technology has necessitated the use of a systematic and reliable method to take advantage of such data for flux analysis. Here we applied a nonlinear, optimization-based method to study substrate metabolism in cardiomyocytes using (13)C data from p...

journal_title：Biotechnology and bioengineering

authors： Vo TD,Palsson BO

更新日期：2006-12-05 00:00:00

abstract：:Buffered aqueous two-phase systems are effective extraction systems for separating amphoteric hydrocarbons like, for example, polypeptides from aqueous phases. The design and basic engineering of such processes requires the knowledge of the liquid-liquid equilibrium. The study presented here aims to contribute to the ...

journal_title：Biotechnology and bioengineering

authors： Grossmann C,Tintinger R,Zhu J,Maurer G

更新日期：1998-12-20 00:00:00

abstract：:In this study we report the isolation of four denitrifying bacteria from a batch reactor, where the progress of hydrogenotrophic denitrification was examined. Only three of the strains had the ability to use hydrogen as electron donor. In the present work, kinetic batch experiments were carried out in order to study t...

journal_title：Biotechnology and bioengineering

authors： Vasiliadou IA,Siozios S,Papadas IT,Bourtzis K,Pavlou S,Vayenas DV

更新日期：2006-10-20 00:00:00

abstract：:Membrane chromatography (MC) systems are finding increasing use in downstream processing trains for therapeutic proteins due to the unique mass-transfer characteristics they provide. As a result, there is increased need for model-based methods to scale-up MC units using data collected on a scaled-down unit. Here, a st...

journal_title：Biotechnology and bioengineering

authors： Ghosh P,Lin M,Vogel JH,Choy D,Haynes C,von Lieres E

更新日期：2014-08-01 00:00:00

abstract：:The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...

journal_title：Biotechnology and bioengineering

authors： Shimizu H,Araki K,Shioya S,Suga K

更新日期：1991-06-20 00:00:00

abstract：:An experimental device was constructed to allow nearly simultaneous measurements to be made on temperature and gas composition at different depths in a solid-substrate fermentation bed. The time-dependent values of temperature, mol % O(2) and mol % CO(2) were measured at five positions in beds 6.35 cm (2.5 in.) deep. ...

journal_title：Biotechnology and bioengineering

authors： Rathbun BL,Shuler ML

更新日期：1983-04-01 00:00:00

abstract：:At the laboratory scale, sudden step increases from 30 to 42 degrees C can be readily accomplished when expressing heterologous proteins in heat-inducible systems. However, for large scale-cultures only slow ramp-type increases in temperature are possible due to heat transfer limitations, where the heating rate decrea...

journal_title：Biotechnology and bioengineering

authors： Caspeta L,Flores N,Pérez NO,Bolívar F,Ramírez OT

更新日期：2009-02-01 00:00:00

abstract：:A specially designed model reactor based on a 42-L laboratory fermentor was equipped with six stirrers (Rushton turbines) and five cylindrical disks. In this model reactor, the mixing time, Theta(90), turned out to be 13 times longer compared with the 42-L standard laboratory fermentor fitted with two Rushton turbines...

journal_title：Biotechnology and bioengineering

authors： Schilling BM,Pfefferle W,Bachmann B,Leuchtenberger W,Deckwer W

更新日期：1999-09-05 00:00:00

abstract：:A three-pool growth model of an individual Escherichia coli cell is described herein. The model is based on a previously developed chemically structured complex single cell growth model. The reduction in model complexity and the identification of the essential modes of motion, over the time scale of growth, is achieve...

journal_title：Biotechnology and bioengineering

authors： Joshi A,Palsson BO

更新日期：1988-02-05 00:00:00

abstract：:The gp41 polypeptide of human immunodeficiency virus (HIV) contains an immunosuppressive domain, an epitope which elicits specific cytolytic T cell responses to HIV, and a complement Clq interactive domain. In addition, a synthetic peptide called CS3, derived from gp41 (amino acids 576-593 of gp160) and contiguous wit...

journal_title：Biotechnology and bioengineering

authors： Kestler DP,Henderson LA,Noti JD

更新日期：1993-06-05 00:00:00

abstract：:Based on batch cultivation data for the production of L-glutamic acid from glucose, a comparative evaluation was made for repeated batch cultivations using one and more fermentors. The problem was formulated as maximizing the productivity of metabolic product with the specified conversion with respect to the cell age ...

journal_title：Biotechnology and bioengineering

authors： Shimizu K,Kobayashi T,Nagara A,Matsubara M

更新日期：1985-05-01 00:00:00

abstract：:Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO(2) NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction...

journal_title：Biotechnology and bioengineering

authors： Chen P,Kanehira K,Sonezaki S,Taniguchi A

更新日期：2012-12-01 00:00:00

abstract：:A model that was used to describe toxicity from high concentrations of chlorinated aliphatic hydrocarbons (CAHs) on reductively dechlorinating cultures in batch reactors (Sabalowsky and Semprini (in press)) was extended here to simulate observations in continuous flow suspended and attached growth reactors. The reduct...

journal_title：Biotechnology and bioengineering

authors： Sabalowsky AR,Semprini L

更新日期：2010-10-15 00:00:00

abstract：:The kinetic behavior of soluble and insoluble forms of dextransucrase from Leuconostoc mesenteroides NRRL B-1299 was investigated with sucrose as substrate and maltose as acceptor. To study the parameters involved, a kinetic model was applied that was previously developed for L. mesenteroides NRRL B-512F dextransucras...

journal_title：Biotechnology and bioengineering

authors： Dols M,Remaud-Simeon M,Willemot RM,Demuth B,Jordening HJ,Buchholz K,Monsan P

更新日期：1999-05-05 00:00:00

abstract：:The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...

journal_title：Biotechnology and bioengineering

authors： Petersen LAH,Lieven C,Nandy SK,Villadsen J,Jørgensen SB,Christensen I,Gernaey KV

更新日期：2019-11-01 00:00:00

abstract：:The initial steps of glycerol dissimilation and 1,3-propanediol (1, 3-PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during transient phases. The...

journal_title：Biotechnology and bioengineering

authors： Ahrens K,Menzel K,Zeng A,Deckwer W

更新日期：1998-09-05 00:00:00

abstract：:An in vitro toxicity testing system that combines perfusion of a membrane-supported tissue model with measurement of trans-epithelial electrical resistance (TER) is described. The system is fully automated and allows for continuous TER measurements during exposure to a test agent. The utility of this system is demonst...

journal_title：Biotechnology and bioengineering

authors： Pasternak AS,Miller WM

更新日期：1996-06-05 00:00:00

abstract：:Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids of chain lengths from C6 to C16 are propitious intermediates for the catalytic synthesis of industrial chemicals and diesel-like biofuels. The abundance of gen...

journal_title：Biotechnology and bioengineering

authors： Tee TW,Chowdhury A,Maranas CD,Shanks JV

更新日期：2014-05-01 00:00:00

abstract：:Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Caram HS,Humphrey AE

更新日期：1984-08-01 00:00:00

abstract：:The accumulation of inorganic phosphate inhibits protein synthesis in cell-free protein synthesis reactions that are energized by high-energy-phosphate-containing compounds. This study developed a new scheme for supplying energy using dual energy sources to enhance the regeneration of ATP and lower the rate of phospha...

journal_title：Biotechnology and bioengineering

authors： Kim TW,Oh IS,Keum JW,Kwon YC,Byun JY,Lee KH,Choi CY,Kim DM

更新日期：2007-08-15 00:00:00

abstract：:Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation. In the latter case, trapping efficiencies of up to 100% could be obtained. Activities ...

journal_title：Biotechnology and bioengineering

authors： Mosmuller EW,Franssen MC,Engbersen JF

更新日期：1993-06-20 00:00:00

abstract：:Trypsin inhibitor was converted to hydrophobic states by covalently combining cholesteryl groups using an acylation reaction, and was immobilized in reverse micelles composed of a nonionic surfactant. Using this reverse micellar phase containing trypsin inhibitor as an affinity ligand, trypsin was selectively separate...

journal_title：Biotechnology and bioengineering

authors： Adachi M,Yamazaki M,Harada M,Shioi A,Katch S

更新日期：1997-02-20 00:00:00

abstract：:Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

journal_title：Biotechnology and bioengineering

authors： Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

更新日期：2006-02-20 00:00:00

abstract：:Membrane morphology is compared to protein depostion under passive adsorption and ultrafiltration conditions. Solute resistance of protein deposits for membranes of varying roughness, structure, and permeability can vary dramatically with operating conditions. Using Brunauer-Emmett-Teller adsorption isotherm (BET), st...

journal_title：Biotechnology and bioengineering

authors： Chen V,Kim KJ,Fane AG

更新日期：1995-07-20 00:00:00

abstract：:Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disu...

journal_title：Biotechnology and bioengineering

authors： Baac H,Hajós JP,Lee J,Kim D,Kim SJ,Shuler ML

更新日期：2006-07-05 00:00:00