Activation of lignin peroxidase in organic media by reversed micelles.

Abstract:

:Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined in this study; however, LIP was catalytically active by being entrapped in the AOT reversed micellar solution. LIP activity in the reversed micelle was enhanced by optimizing either the preparation or the operation conditions, such as water content and pH in water pools of the reversed micelle and the reaction temperature. Stable activity was obtained in isooctane because of the stability of the reversed micelle. The optimal pH was 5 in the reversed micellar system, which shifted from pH 3 in the aqueous solution. The degradation reaction of several environmental pollutants was attempted using LIP hosted in the AOT reversed micelle. Degradation achieved after a 1-h reaction reached 81%, 50%, and 22% for p-nonylphenol, bisphenol A, and 2,4-dichlorophenol, respectively. This is the first report on the utilization of LIP in organic media.

journal_name

Biotechnol Bioeng

authors

Kimura M,Michizoe J,Oakazaki SY,Furusaki S,Goto M,Tanaka H,Wariishi H

doi

10.1002/bit.20277

subject

Has Abstract

pub_date

2004-11-20 00:00:00

pages

495-501

issue

4

eissn

0006-3592

issn

1097-0290

journal_volume

88

pub_type

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