Activation of lignin peroxidase in organic media by reversed micelles.


:Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined in this study; however, LIP was catalytically active by being entrapped in the AOT reversed micellar solution. LIP activity in the reversed micelle was enhanced by optimizing either the preparation or the operation conditions, such as water content and pH in water pools of the reversed micelle and the reaction temperature. Stable activity was obtained in isooctane because of the stability of the reversed micelle. The optimal pH was 5 in the reversed micellar system, which shifted from pH 3 in the aqueous solution. The degradation reaction of several environmental pollutants was attempted using LIP hosted in the AOT reversed micelle. Degradation achieved after a 1-h reaction reached 81%, 50%, and 22% for p-nonylphenol, bisphenol A, and 2,4-dichlorophenol, respectively. This is the first report on the utilization of LIP in organic media.


Biotechnol Bioeng


Kimura M,Michizoe J,Oakazaki SY,Furusaki S,Goto M,Tanaka H,Wariishi H




Has Abstract


2004-11-20 00:00:00












  • H2 production by photofermentation in an innovative plate-type photobioreactor with meandering channels.

    abstract::Hydrogen production by Rhodobacter capsulatus is an anaerobic, photobiological process requiring specific mixing conditions. In this study, an innovative design of a photobioreactor is proposed. The design is based on a plate-type photobioreactor with an interconnected meandering channel to allow culture mixing and H2...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Turon V,Ollivier S,Cwicklinski G,Willison JC,Anxionnaz-Minvielle Z

    更新日期:2020-12-15 00:00:00

  • Novel electrochemical-enzymatic model which quantifies the effect of the solution Eh on the kinetics of ferrous iron oxidation with Acidithiobacillus ferrooxidans.

    abstract::The influence of solution Eh on the rate of ferrous iron oxidation by Acidithiobacillus ferrooxidans is characterized. The experimental approach was based on the use of a two-chamber bioelectrochemical cell, which can determine the ferrous iron oxidation rate at controlled potential. Results enabled the formulation of...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Meruane G,Salhe C,Wiertz J,Vargas T

    更新日期:2002-11-05 00:00:00

  • Analysis of metabolic networks of Streptomyces leeuwenhoekii C34 by means of a genome scale model: Prediction of modifications that enhance the production of specialized metabolites.

    abstract::The first genome scale model (GSM) for Streptomyces leeuwenhoekii C34 was developed to study the biosynthesis pathways of specialized metabolites and to find metabolic engineering targets for enhancing their production. The model, iVR1007, consists of 1,722 reactions, 1,463 metabolites, and 1,007 genes, it includes th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Razmilic V,Castro JF,Andrews B,Asenjo JA

    更新日期:2018-07-01 00:00:00

  • Use of lipophilic anions for estimation of biomass and cell viability.

    abstract::A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB(-)) to the cells. This method can also be used for the analysis of a complex population ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Daugelavicius R,Bakiene E,Berzinskiene J,Bamford DH

    更新日期:2000-01-01 00:00:00

  • Trehalase immobilization on aminopropyl glass for analytical use.

    abstract::Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

    更新日期:1997-04-05 00:00:00

  • Cellulase immobilization on Fe3O4 and characterization.

    abstract::A successful procedure for attaching cellulase to 45 microm iron oxide particles with a high-molecular-weight (1000) ligand has been developed. Mass and activity balances were calculated for immobilized enzymes with different loadings. The highest specific activity of the immobilized enzyme was 5.9 mmol glucose/g boun...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Garcia A 3rd,Oh S,Engler CR

    更新日期:1989-01-15 00:00:00

  • Oxygen-responsive genetic circuits constructed in Synechocystis sp. PCC 6803.

    abstract::As photoautotrophic prokaryotes, cyanobacteria are promising platforms for producing value-added bioproducts. However, few regulatory genetic parts and devices (e.g., inducible promoters and regulatory circuits) have been developed for these potential hosts. Furthermore, the devices that have been created respond only...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Immethun CM,Ng KM,DeLorenzo DM,Waldron-Feinstein B,Lee YC,Moon TS

    更新日期:2016-02-01 00:00:00

  • Increased recombinant protein production in Escherichia coli strains with overexpressed water-forming NADH oxidase and a deleted ArcA regulatory protein.

    abstract::Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vemuri GN,Eiteman MA,Altman E

    更新日期:2006-06-20 00:00:00

  • Comparative studies on enzyme preparations and role of cell components for (R)-phenylacetylcarbinol production in a two-phase biotransformation.

    abstract::Whole cell pyruvate decarboxylase (PDC) from Candida utilis enhanced the enzymatic production of (R)-phenylacetylcarbinol (PAC) in an aqueous/octanol biotransformation compared to the partially purified PDC especially for a lower range of initial activities (0.3-2.5 U/mL). With an initial activity of 1.1 U/mL and at a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Satianegara G,Rogers PL,Rosche B

    更新日期:2006-08-20 00:00:00

  • Utilization of a palladium-metal oxide semiconductor (Pd-MOS) sensor for on-line monitoring of dissolved hydrogen in anaerobic digestion.

    abstract::The use of a hydrogen-sensitive palladium-metal oxide semiconductor (Pd-MOS) sensor in combination with a membrane for liquid-to-gas transfer for the detection of dissolved hydrogen was investigated. The system was evaluated with known concentrations of dissolved hydrogen in water. The lowest concentration detected wi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Björnsson L,Hörnsten EG,Mattiasson B

    更新日期:2001-04-05 00:00:00

  • Part II. Overexpression of bcl-2 family members enhances survival of mammalian cells in response to various culture insults.

    abstract::A number of bioreactor configurations have been developed for the manufacture of products from mammalian cell hosts. Even in the most efficient of these, however, problems such as nutrient exhaustion, growth factor deprivation, and toxin accumulations may arise. Consequently, the current effort focused on the feasibil...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mastrangelo AJ,Hardwick JM,Zou S,Betenbaugh MJ

    更新日期:2000-03-05 00:00:00

  • Biocatalytic process optimization based on mechanistic modeling of cholic acid oxidation with cofactor regeneration.

    abstract::Reduction and oxidation of steroids in the human gut are catalyzed by hydroxysteroid dehydrogenases of microorganisms. For the production of 12-ketochenodeoxycholic acid (12-Keto-CDCA) from cholic acid the biocatalytic application of the 12α-hydroxysteroid dehydrogenase of Clostridium group P, strain C 48-50 (HSDH) is...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Braun M,Link H,Liu L,Schmid RD,Weuster-Botz D

    更新日期:2011-06-01 00:00:00

  • Live cell imaging compatible immobilization of Chlamydomonas reinhardtii in microfluidic platform for biodiesel research.

    abstract::This paper describes a novel surface immobilization method for live-cell imaging of Chlamydomonas reinhardtii for continuous monitoring of lipid droplet accumulation. Microfluidics allows high-throughput manipulation and analysis of single cells in precisely controlled microenvironment. Fluorescence imaging based quan...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Park JW,Na SC,Nguyen TQ,Paik SM,Kang M,Hong D,Choi IS,Lee JH,Jeon NL

    更新日期:2015-03-01 00:00:00

  • Evaluation of selectivity changes in HIC systems using a preferential interaction based analysis.

    abstract::It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xia F,Nagrath D,Garde S,Cramer SM

    更新日期:2004-08-05 00:00:00

  • Production and characterization of liquid-core capsules made from cross-linked acrylamide copolymers for biotechnological applications.

    abstract::A novel chemistry has been developed for the production of capsules composed of a hydrophobic liquid core surrounded by a cross-linked polyacrylamide/alginate membrane. These liquid-core capsules may be used in capsular perstraction for the removal of inhibitory products from bioprocesses and bioconversions. They have...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wyss A,von Stockar U,Marison IW

    更新日期:2004-06-05 00:00:00

  • Random mutagenesis of global transcription factor cAMP receptor protein for improved osmotolerance.

    abstract::The naturally existing microbial hosts can rarely satisfy industrial requirements, thus there has always been an intense effort in strain engineering to meet the needs of these bioprocesses. Here, in this work, we want to prove the concept that engineering global transcription factor cAMP receptor protein (CRP) of Esc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang H,Chong H,Ching CB,Jiang R

    更新日期:2012-05-01 00:00:00

  • High cell density and high monoclonal antibody production through medium design and rational control in a bioreactor.

    abstract::A simple feeding strategy was developed and successfully employed for nutritional control in a 2-L fed-batch culture of hybridoma cells. A previously developed stoichiometric model for animal cell growth was used to design a supplemental medium for feeding. Undialyzed fetal bovine serum and trace metals (Fe(2+), SeO(3...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xie L,Wang DI

    更新日期:1996-09-20 00:00:00

  • Self-renaturing enzymes: design of an enzyme-chaperone chimera as a new approach to enzyme stabilization.

    abstract::Molecular chaperones in aqueous-organic mixtures can broaden the utility of biocatalysis by stabilizing enzymes in denaturing conditions. We have designed a self-renaturing enzyme-chaperone chimera consisting of penicillin amidase and a thermophilic chaperonin that functions in aqueous-organic mixtures. The flexible l...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bergeron LM,Gomez L,Whitehead TA,Clark DS

    更新日期:2009-04-01 00:00:00

  • Fed-batch culture of Bacillus thuringiensis for thuringiensin production in a tower type bioreactor.

    abstract::Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose c...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jong JZ,Hsiun DY,Wu WT,Tzeng YM

    更新日期:1995-11-05 00:00:00

  • Apparent zero-order kinetics of phenol biodegradation by substrate-inhibited microbes at low substrate concentrations.

    abstract::The reaction kinetics for phenol biodegradation at low substrate concentrations can be estimated based on the analysis of changes in the dissolved oxygen concentration in the bulk liquid during biodegradation. The measured oxygen concentration changes with an interesting behavior as biodegradation proceeds. The oxygen...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shishido M,Toda M

    更新日期:1996-06-20 00:00:00

  • Diversity matters: dynamic simulation of distributed bacterial states in suspended growth biological wastewater treatment systems.

    abstract::A MATLAB-based program was developed to simulate the distributions of states and behaviors of microbial storage product-accumulating bacteria in suspended growth systems. Currently available computer simulators of these systems predict dynamic behaviors by numerically solving differential biokinetic equations using av...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schuler AJ

    更新日期:2005-07-05 00:00:00

  • EGFR-targeted Chimeras of Pseudomonas ToxA released into the extracellular milieu by attenuated Salmonella selectively kill tumor cells.

    abstract::Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Quintero D,Carrafa J,Vincent L,Bermudes D

    更新日期:2016-12-01 00:00:00

  • Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis.

    abstract::Metabolic carbon labelling experiments enable a large amount of extracellular fluxes and intracellular carbon isotope enrichments to be measured. Since the relation between the measured quantities and the unknown intracellular metabolic fluxes is given by bilinear balance equations, flux determination from this data s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wiechert W,Siefke C,de Graaf AA,Marx A

    更新日期:1997-07-05 00:00:00

  • In situ determination of kinetic parameters for biofilms: Isolation and characterization of oligotrophic biofilms.

    abstract::This article develops and utilizes an in situ technique to estimate the Monod half-maximum rate concentration, K(s) and the maximum specific utilization rate constant, k, for biofilms. The technique employs a curve-matching method with kinetic results from several short-term experiments with completely mixed biofilm r...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rittmann BE,Crawford L,Tuck CK,Namkung E

    更新日期:1986-11-01 00:00:00

  • Selective adsorption of plant products.

    abstract::The results from this study demonstrate that neutral resins can selectively concentrate specific indole alkaloids from dilute aqueous mixtures. Adsorption was observed to provide a one to two order of magnitude improvement in concentrating these alkaloids, as compared to an equivalent single-staged extraction. Since t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Payne GF,Shuler ML

    更新日期:1988-06-05 00:00:00

  • Nitrogen polishing in a fully anoxic anammox MBBR treating mainstream nitritation-denitritation effluent.

    abstract::As nitrogen discharge limits are becoming more stringent, short-cut nitrogen systems and tertiary nitrogen polishing steps are gaining popularity. For partial nitritation or nitritation-denitritation systems, anaerobic ammonia oxidation (anammox) polishing may be feasible to remove residual ammonia and nitrite from th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Regmi P,Holgate B,Miller MW,Park H,Chandran K,Wett B,Murthy S,Bott CB

    更新日期:2016-03-01 00:00:00

  • Algal autoflocculation--verification and proposed mechanism.

    abstract::Biomass autoflocculation in outdoor algal cultures was found to be associated with increases of culture pH levels, due to CO(2) consumption by the algal photosynthetic activity. Under these alkaline conditions, some medium chemical ions precipitated together with the algal biomass. The chemical substances involved wit...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sukenik A,Shelef G

    更新日期:1984-02-01 00:00:00

  • Kinetics of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions.

    abstract::This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang QQ,Zhu JY,Hunt CG,Zhan HY

    更新日期:2012-08-01 00:00:00

  • Application of temperature-sensitive mutants for single-cell protein production.

    abstract::Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Miyasaka Y,Rha C,Sinskey AJ

    更新日期:1980-10-01 00:00:00

  • Studies on the stability of immobilized xanthine oxidase and urate oxidase.

    abstract::The stability of immobilized preparations of xanthine oxidase and urate oxidase was studied, and optimized, because of the potential joint use of both enzymes in clinical analysis. Xanthine oxidase was immobilized on cellulose, Sepharose, hornblende, Enzacryl-TIO, and porous glass. Thehalf-lives of these preparations ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Johnson DB,Coughlan MP

    更新日期:1978-07-01 00:00:00