A continuous membrane bioreactor for ester synthesis in organic media: I. Operational characterization and stability.

abstract：:As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...

journal_title：Biotechnology and bioengineering

authors： Calikoglu Koyuncu AC,Nayman AH,Telci D,Torun Kose G

更新日期：2020-06-01 00:00:00

abstract：:The use of microalgae for biofuel production will be beneficial to society if we can produce biofuels at large scales with minimal mechanical energy input in the production process. Understanding micro-algal physiological responses under variable environmental conditions in bioreactors is essential for the optimizatio...

journal_title：Biotechnology and bioengineering

authors： Hansen TJ,Hondzo M,Mashek MT,Mashek DG,Lefebvre PA

更新日期：2013-01-01 00:00:00

abstract：:The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved ...

journal_title：Biotechnology and bioengineering

authors： Ménil S,Petit JL,Courvoisier-Dezord E,Debard A,Pellouin V,Reignier T,Sergent M,Deyris V,Duquesne K,de Berardinis V,Alphand V

更新日期：2019-11-01 00:00:00

abstract：:The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) strongly reduces their bioavailability in aged contaminated soils, thus limiting their bioremediation. The biodegradation of PAHs in soils can be enhanced by employing surface-active agents. However, chemical surfactants are often recalcitrant and exer...

journal_title：Biotechnology and bioengineering

authors： Fava F,Berselli S,Conte P,Piccolo A,Marchetti L

更新日期：2004-10-20 00:00:00

abstract：:This article deals with the modeling of the oxygen transfer in an industrial airlift fermentor used for lactic yeast production on whey substrates. The purpose of this study was to improve the understanding of the interactions among the various parameters that govern the oxygen transfer phenomena in this type of ferme...

journal_title：Biotechnology and bioengineering

authors： Pigache S,Dhoms P,Trystram G

更新日期：1992-04-15 00:00:00

abstract：:In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

journal_title：Biotechnology and bioengineering

authors： van der Pol L,Bakker WA,Tramper J

更新日期：1992-06-05 00:00:00

abstract：:Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (R...

journal_title：Biotechnology and bioengineering

authors： Ritter A,Rauschert T,Oertli M,Piehlmaier D,Mantas P,Kuntzelmann G,Lageyre N,Brannetti B,Voedisch B,Geisse S,Jostock T,Laux H

更新日期：2016-11-01 00:00:00

abstract：:Many sites of environmental concern contain groundwater contaminated with nonaqueous phase liquids (NAPL). In such sites interfacial processes may affect both the equilibrium and kinetic behavior of the system. In particular, insoluble hydrocarbon partitioning and microbial biodegradation of insoluble hydrocarbon are ...

journal_title：Biotechnology and bioengineering

authors： Sekelsky AM,Shreve GS

更新日期：1999-05-20 00:00:00

abstract：:The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...

journal_title：Biotechnology and bioengineering

authors： Beun JJ,Heijnen JJ,van Loosdrecht MC

更新日期：2001-10-05 00:00:00

abstract：:Cellulase was modified with amphilic copolymers made of alpha-allyl-omega-methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer....

journal_title：Biotechnology and bioengineering

authors： Park JW,Kajiuchi T

更新日期：1995-02-20 00:00:00

abstract：:Autoflocculation and bioflocculation are considered to be the most promising means for the economical harvesting of microalgae. We have therefore studied these phenomena with cultures of Scenedesmus obliquus produced during biological tertiary wastewater treatment. The quantity of extracellular polymers produced durin...

journal_title：Biotechnology and bioengineering

authors： Lavoie A,de la Noüe J

更新日期：1987-11-01 00:00:00

abstract：:Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...

journal_title：Biotechnology and bioengineering

authors： Li SW,Yu B,Byrne G,Wright M,O'Rourke S,Mesa K,Berman PW

更新日期：2019-09-01 00:00:00

abstract：:A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

journal_title：Biotechnology and bioengineering

authors： Wang M,Yu C,Zhao H

更新日期：2016-03-01 00:00:00

abstract：:Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...

journal_title：Biotechnology and bioengineering

authors： Kim Y,Arp DJ,Semprini L

更新日期：2002-12-05 00:00:00

abstract：:The S-System formalism provides a popular, versatile and mathematically tractable representation of metabolic pathways. At steady-state, after a logarithmic transformation, the S-System representation reduces into a system of linear equations. Thus, the maximization of a particular metabolite concentration or a flux s...

journal_title：Biotechnology and bioengineering

authors： Petkov SB,Maranas CD

更新日期：1997-10-20 00:00:00

abstract：:The anaerobic digestion of a cellulose-enriched fraction of domestic refuse by means of rumen microorganisms in an "artificial rumen" digester was studied. Various combinations of solid and liquid retention times and loading rates were applied to establish optimum conditions for the acidogenic phase digestion of the r...

journal_title：Biotechnology and bioengineering

authors： Gijzen HJ,Zwart KB,Teunissen MJ,Vogels GD

更新日期：1988-09-05 00:00:00

abstract：:Mucopolysaccharidosis (MPS) Type II is caused by mutations in the gene encoding the lysosomal enzyme, iduronate 2-sulfatase (IDS). The majority of MPSII cases affect the brain. However, enzyme replacement therapy with recombinant IDS does not treat the brain, because IDS is a large molecule drug that does not cross th...

journal_title：Biotechnology and bioengineering

authors： Boado RJ,Ka-Wai Hui E,Zhiqiang Lu J,Pardridge WM

更新日期：2014-11-01 00:00:00

abstract：:The thickness variability of biofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, and the binary population combination of these two species was quantified. The experimental method involved cryoembedding biofilms with a commercial tissue embedding agent, sectioning, and applying image analysis to construct thick...

journal_title：Biotechnology and bioengineering

authors： Murga R,Stewart PS,Daly D

更新日期：1995-03-20 00:00:00

abstract：:A comparative study of the performance of solid and liquid non-aqueous phases (NAPs) to enhance the mass transfer and biodegradation of hexane by Pseudomonas aeruginosa in two-phase partitioning bioreactors (TPPBs) was undertaken. A preliminary NAP screening was thus carried out among the most common solid and liquid ...

journal_title：Biotechnology and bioengineering

authors： Hernández M,Quijano G,Thalasso F,Daugulis AJ,Villaverde S,Muñoz R

更新日期：2010-08-01 00:00:00

abstract：:Pseudomonas putida cells were grown in confined volumes in dual-membrane immobilized cell reactors constructed from microporous polyethylene hollow fibers and silicone rubber tubules as a model system for the study of mass transport in microbial aggregates. Local cell concentrations in the reactors reached 300 g dry m...

journal_title：Biotechnology and bioengineering

authors： Karel SF,Robertson CR

更新日期：1989-07-01 00:00:00

abstract：:Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids of chain lengths from C6 to C16 are propitious intermediates for the catalytic synthesis of industrial chemicals and diesel-like biofuels. The abundance of gen...

journal_title：Biotechnology and bioengineering

authors： Tee TW,Chowdhury A,Maranas CD,Shanks JV

更新日期：2014-05-01 00:00:00

abstract：:In analyzing microbial cellulose utilization, it would be useful to independently measure the mass concentration of cells and cellulase enzymes. Such measurements would allow investigation of the allocation of cellular resources between synthesis of cells and cellulase, in vivo cell- and cellulase-specific cellulose h...

journal_title：Biotechnology and bioengineering

authors： Lynd LR,Zhang Y

更新日期：2002-02-15 00:00:00

abstract：:Using our recently developed sensor reactor approach, lysine-producing, nongrowing Corynebacterium glutamicum MH20-22B cells were subjected to serial (13)C-labeling experiments for flux analysis during the leucine-limited fed-batch production phase in a 300-L bioreactor. Based on two-dimensional (2D) nuclear magnetic ...

journal_title：Biotechnology and bioengineering

authors： Drysch A,El Massaoudi M,Wiechert W,de Graaf AA,Takors R

更新日期：2004-03-05 00:00:00

abstract：:A fuzzy-logic light attenuation model was developed and validated for a stirred-tank photobioreactor. Based on this model, local light intensities were used to calculate local specific growth rates of the cyanobacteria Synechococcus PCC 7942. The light regime for maximization of biomass space-time yield in a batch pro...

journal_title：Biotechnology and bioengineering

authors： Franco-Lara E,Havel J,Peterat F,Weuster-Botz D

更新日期：2006-12-20 00:00:00

abstract：:Three-dimensional (3D) tumor models have been established in various microfluidic systems for drug delivery and resistance studies in vitro. However, one of the main drawbacks of these models is non-uniform distribution of cells, leaving regions with very low cell density within the 3D structures. As a result, molecul...

journal_title：Biotechnology and bioengineering

authors： Elliott NT,Yuan F

更新日期：2012-05-01 00:00:00

abstract：:Conventional wet chemistry methods to determine biomass composition are labor- and time-intensive and require larger amounts of biomass (300 mg) than is often available. To overcome these limitations and to support a high-throughput pretreatment and hydrolysis (HTPH) screening system, this article reports on the devel...

journal_title：Biotechnology and bioengineering

authors： DeMartini JD,Studer MH,Wyman CE

更新日期：2011-02-01 00:00:00

abstract：:Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy i...

journal_title：Biotechnology and bioengineering

authors： Martín Pérez L,Benítez Casanova L,Moreno Pérez AJ,Pérez Gómez D,Gavaldá Martín S,Ledesma-García L,Valbuena Crespo N,Díez García B,Reyes-Sosa FM

更新日期：2017-11-01 00:00:00

abstract：:In glutamic acid fermentation, the molasses feeding policy and time of penicillin addition significantly affected glutamic acid production, and a fuzzy supervisory control system was developed for their quasi-optimal regulation.From the trend of the experimental data, production rules and membership functions of fuzzy...

journal_title：Biotechnology and bioengineering

authors： Kitsuta Y,Kishimoto M

更新日期：1994-06-05 00:00:00

abstract：:Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening and to provide useful information about various cell-chemical interactions. A computer aided an...

journal_title：Biotechnology and bioengineering

authors： Li XM,Liang BS,Wang HY

更新日期：1988-02-20 00:00:00

abstract：:Human tissue-type plasminogen activator (t-PA) contains a variably occupied glycosylation site at Asn-184 in naturally produced t-PA and in t-PA produced in recombinant Chinese hamster ovary (CHO) cells. The presence of an oligosaccharide at this site has previously been shown to reduce specific activity and fibrin bi...

journal_title：Biotechnology and bioengineering

authors： Andersen DC,Bridges T,Gawlitzek M,Hoy C

更新日期：2000-10-05 00:00:00