Abstract:
:Low-temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using glutaraldehyde as a linking agent. Enzyme activity was evaluated by the response of glucose sensor composed of the immobilized enzyme membrane and a dissolved oxygen electrode. The sensor response was found to depend on the kind of carrier membrane and to become maximum at suitable conditions of plasma treatment.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Kawakami M,Koya H,Gondo Sdoi
10.1002/bit.260320316subject
Has Abstractpub_date
1988-07-20 00:00:00pages
369-73issue
3eissn
0006-3592issn
1097-0290journal_volume
32pub_type
杂志文章abstract::Benzil was reduced stereospecifically to (S)-benzoin by Bacillus cereus strain Tim-r01. To isolate the gene responsible for asymmetric reduction, we constructed a library consisting of Escherichia coli clones that harbored plasmids expressing Bacillus cereus genes. The library was screened using the halo formation ass...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1191
更新日期:2001-12-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25946
更新日期:2016-08-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270311
更新日期:1985-03-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21031
更新日期:2006-10-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280905
更新日期:1986-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260440511
更新日期:1994-08-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1993-08-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390312
更新日期:1992-02-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26472
更新日期:2018-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2002-01-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19981105)60:3<283::aid-bit
更新日期:1998-11-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10826
更新日期:2003-12-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24413
更新日期:2012-06-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2000-10-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27504
更新日期:2020-07-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21080
更新日期:2006-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390711
更新日期:1992-03-25 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2003-03-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2002-11-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2007-11-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1144
更新日期:2001-09-20 00:00:00
abstract::Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10166
更新日期:2002-03-30 00:00:00
abstract::Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27016
更新日期:2019-09-01 00:00:00
abstract::The main processes involved in enhanced biological phosphorus removal (EBPR) under anaerobic and subsequently aerobic conditions are widely described in the literature. Polyphosphate accumulating organisms (PAO) are the organisms responsible for this process. However, the mechanisms of PAO are not fully established ye...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10868
更新日期:2004-03-30 00:00:00
abstract::It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25912
更新日期:2016-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400115
更新日期:1992-06-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1996-02-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260381106
更新日期:1991-12-20 00:00:00