Fluorescent silica particles for monitoring oxygen levels in three-dimensional heterogeneous cellular structures.

abstract：:Downstream processing operations are often carried out blind in the process timescale since product monitoring on-line is not common. Knowledge of the location and concentration of the product and key contaminants is complementary to other process information for process development and, if available on-line in conjun...

journal_title：Biotechnology and bioengineering

authors： Holwill IJ,Chard SJ,Flanagan MT,Hoare M

更新日期：1997-01-05 00:00:00

abstract：:In this study, the fragmentation activities of Thermomonospora fusca cellulases E(2), E(3), E(5), Trichoderma reesei CBHI, and their mixtures were measured to study synergism in fragmentation. Fragmentation studies revealed that only two pure cellulases, T. fusca E(2) and E(5) had significant fragmentation activity. T...

journal_title：Biotechnology and bioengineering

authors： Walker LP,Wilson DB,Irvin DC,McQuire C,Price M

更新日期：1992-11-01 00:00:00

abstract：:A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs...

journal_title：Biotechnology and bioengineering

authors： O'Connell PJ,Varley J

更新日期：2001-08-05 00:00:00

abstract：:Batch fermentations of glucose to ethanol by Z. Mobilis.(ATCC 10988) were examined in several semidefined nutrient media. The measurement of acid produced by the microorganism was used to study its transient fermentation characteristics. Limitation of nitrogen source in the semidefined medium of Rogers and coworkers(2...

journal_title：Biotechnology and bioengineering

authors： Veeramallu U,Agrawal P

更新日期：1988-05-20 00:00:00

abstract：:Immobilized enzyme hollow fibers may be useful in the purification or treatment of whole blood under clinical conditions. In this study, catalytically pure heparinase was immobilized to cellulose to analyze the feasibility for the removal of heparin's anticoagulant activity from whole blood. The kinetics of catalytica...

journal_title：Biotechnology and bioengineering

authors： Comfort AR,Albert E,Langer R

更新日期：1989-12-20 00:00:00

abstract：:The effect of trehalose (0.5 M) on the thermal stability of cutinase in the alkaline pH range was studied. The thermal unfolding induced by increasing temperature was analyzed in the absence and in the presence of trehalose according to a two-state model (which assumes that only the folded and unfolded states of cutin...

journal_title：Biotechnology and bioengineering

authors： Baptista RP,Cabral JM,Melo EP

更新日期：2000-12-20 00:00:00

abstract：:Many biochemical processes consist of a sequence of operations for which optimal operating conditions (setpoints) have to be determined. If such optimization is performed for each operation separately with respect to objectives defined for each operation individually, overall process performance is likely to be subopt...

journal_title：Biotechnology and bioengineering

authors： Groep ME,Gregory ME,Kershenbaum LS,Bogle ID

更新日期：2000-02-05 00:00:00

abstract：:A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...

journal_title：Biotechnology and bioengineering

authors： Corchero JL,Villaverde A

更新日期：1998-06-20 00:00:00

abstract：:Fed-batch cultures of recombinant microorganisms have attracted attention as they can separate cell growth stage from cloned-gene expression phase during fermentations. In this work, the effect of different glucose feeding strategies on cell growth and cloned gene expression was studied during aerobic fed-batch fermen...

journal_title：Biotechnology and bioengineering

authors： Patkar A,Seo JH

更新日期：1992-06-05 00:00:00

abstract：:In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charg...

journal_title：Biotechnology and bioengineering

authors： Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

更新日期：2002-07-20 00:00:00

abstract：:Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...

journal_title：Biotechnology and bioengineering

authors： Dudley BT,Howgrave-Graham AR,Bruton AG,Wallis FM

更新日期：1993-07-01 00:00:00

abstract：:A microarray presenting glycerol nanodroplets of fluorogenic peptide substrates was used as a biosensor for the detection of multiple enzyme activities within human plasma. Using 10 different plasma proteases (kallikrein, factor XIIa, factor XIa, factor IXa, factor VIIa, factor Xa, thrombin, activated protein C, uPA a...

journal_title：Biotechnology and bioengineering

authors： Gosalia DN,Denney WS,Salisbury CM,Ellman JA,Diamond SL

更新日期：2006-08-20 00:00:00

abstract：:Steam explosion after sulphur dioxide impregnation of wood chips is an effective method for improving the enzymatic digestibility of cellulose in the softwood Pinus radiata. Digestibility of pretreated fiber was progressively increased by altering the conditions of steam explosion. With increasing digestibility, there...

journal_title：Biotechnology and bioengineering

authors： Wong KK,Deverell KF,Mackie KL,Clark TA,Donaldson LA

更新日期：1988-04-05 00:00:00

abstract：:One unique to detect cytotoxicity is to utilize reporter gene assays for promoters that respond to stress-induced effects. In the present study, we discovered that the DNA sequence from nt -287 to +110 of the heat shock protein 70B' (HSP70B') gene could be used as a functional promoter to detect cytotoxicity of cadmiu...

journal_title：Biotechnology and bioengineering

authors： Wada K,Taniguchi A,Xu L,Okano T

更新日期：2005-11-20 00:00:00

abstract：:Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a ...

journal_title：Biotechnology and bioengineering

authors： Liu Y,Kim YJ,Siriwon N,Rohrs JA,Yu Z,Wanga P

更新日期：2018-06-01 00:00:00

abstract：:In this study, we assessed the importance of cytoskeleton organization in the mammalian cells used to produce therapeutic proteins. Two cytoskeletal genes, Actin alpha cardiac muscle 1 (ACTC1) and a guanosine triphosphate GTPase-activating protein (TAGAP), were found to be upregulated in highly productive therapeutic ...

journal_title：Biotechnology and bioengineering

authors： Pourcel L,Buron F,Arib G,Le Fourn V,Regamey A,Bodenmann I,Girod PA,Mermod N

更新日期：2020-04-01 00:00:00

abstract：:A stable Tn-5B1-4 insect cell line co-expressing the recombinant GFPuv-beta1,3-N-acetylglucosaminyltransferase 2 (GFPuv-beta3GnT2) protein fused to a melittin signal sequence with a lectin-like molecular chaperone, human calnexin (hCNX) or human calreticulin (hCRT), was constructed. The expression of either of these m...

journal_title：Biotechnology and bioengineering

authors： Kato T,Murata T,Usui T,Park EY

更新日期：2005-02-20 00:00:00

abstract：:Three-dimensional (3D) tumor models have been established in various microfluidic systems for drug delivery and resistance studies in vitro. However, one of the main drawbacks of these models is non-uniform distribution of cells, leaving regions with very low cell density within the 3D structures. As a result, molecul...

journal_title：Biotechnology and bioengineering

authors： Elliott NT,Yuan F

更新日期：2012-05-01 00:00:00

abstract：:Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression-liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. H...

journal_title：Biotechnology and bioengineering

authors： Kamiya K,Tsumoto K,Arakawa S,Shimizu S,Morita I,Yoshimura T,Akiyoshi K

更新日期：2010-12-01 00:00:00

abstract：:Although microalgae are considered as a promising feedstock for biofuels, the energy efficiency of the production process needs to be significantly improved. Due to their small size and low concentration in the culture medium, cost-efficient harvesting of microalgae is a major challenge. In this study, the use of elec...

journal_title：Biotechnology and bioengineering

authors： Vandamme D,Pontes SC,Goiris K,Foubert I,Pinoy LJ,Muylaert K

更新日期：2011-10-01 00:00:00

abstract：:Based on batch cultivation data for the production of L-glutamic acid from glucose, a comparative evaluation was made for repeated batch cultivations using one and more fermentors. The problem was formulated as maximizing the productivity of metabolic product with the specified conversion with respect to the cell age ...

journal_title：Biotechnology and bioengineering

authors： Shimizu K,Kobayashi T,Nagara A,Matsubara M

更新日期：1985-05-01 00:00:00

abstract：:Vascular leak is a key driver of organ injury in diseases, and strategies that reduce enhanced permeability and vascular inflammation are promising therapeutic targets. Activation of the angiopoietin-1 (ANG1)-Tie2 tyrosine kinase signaling pathway is an important regulator of vascular quiescence. Here we describe the ...

journal_title：Biotechnology and bioengineering

authors： Liu P,Ryczko M,Xie X,Baardsnes J,Lord-Dufour S,Duroche Y,Hicks EA,Taiyab A,Sheardown H,Quaggin SE,Jin J

更新日期：2021-01-01 00:00:00

abstract：:Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...

journal_title：Biotechnology and bioengineering

authors： Stone GP,Mernaugh R,Haselton FR

更新日期：2005-09-20 00:00:00

abstract：:After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...

journal_title：Biotechnology and bioengineering

authors： Marek M,Valentová O,Kás J

更新日期：1984-10-01 00:00:00

abstract：:Zwittermicin A (ZwA), a hybrid polyketide- peptide antibiotic, and N-acyl -homoserine-lactonase (AHL-lactonase), a kind of quorum-quenching enzyme, have the potential to prevent the virulence of Erwinia carotovora, which is a major pathogen of soft rot. The purpose of this study was to combine the two agents in one ho...

journal_title：Biotechnology and bioengineering

authors： Zhao C,Zeng H,Yu Z,Sun M

更新日期：2008-06-15 00:00:00

abstract：:Plasmid gene product accumulation in a cell population depends on the fraction of plasmid-containing cells and the distribution of single-cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single-cell leve...

journal_title：Biotechnology and bioengineering

authors： Hjortso MA,Bailey JE

更新日期：1984-05-01 00:00:00

abstract：:Batch hydrolysis kinetics of paper birch (Betula papyrifera) xylan and its associated acetyl groups in dilute sulfuric acid have been measured for acid concentrations of between 0.04 and 0.18M and temperatures of between 100 and 170 degrees C. Only 5% of the cellulose was hydrolyzed for up to 85% xylan removal. Rate d...

journal_title：Biotechnology and bioengineering

authors： Maloney MT,Chapman TW,Baker AJ

更新日期：1985-03-01 00:00:00

abstract：:As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...

journal_title：Biotechnology and bioengineering

authors： Calikoglu Koyuncu AC,Nayman AH,Telci D,Torun Kose G

更新日期：2020-06-01 00:00:00

abstract：:In an attempt to extend and intensify the productive periods of bioprocesses, a self-cleaning tangential filtration device was examined. Built into a special-design bioreactor, its cell retention was evaluated for continuous-flow operation with selected examples of bacteria (Escherichia coli), yeasts (Sacharomyces cer...

journal_title：Biotechnology and bioengineering

authors： Naja G,Volesky B,Schnell A

更新日期：2006-11-05 00:00:00

abstract：:Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce alpha-galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05-0.2 liter/kg/min), rpm (0.15-...

journal_title：Biotechnology and bioengineering

authors： Silman RW

更新日期：1980-02-01 00:00:00