Abstract:
:The catalytic properties of bovine serum albumin (BSA) have been studied rather extensively. The protein has shown catalytic activities toward a variety of reactions in aqueous media. However, there appears to be no reports of reactions catalyzed exclusively in organic media. In this study, we report the catalytic property of BSA on the isomerization of 1,2-benzisoxazole in an organic solvent. Kinetics were performed using a continuous flow system in a spectrophotometer at 330 nm. The catalysis showed a Michaelis-Menten behavior with an estimated catalytic efficiency (k(cat)/K(M)) of 8.77 M(-1)S(-1). The influence of water content and inhibitors was also studied. The activity of BSA in acetonitrile is approximately 20% of that in water under similar conditions. Inhibition studies show that the active site may be the pyridoxal binding site involving Lys-220.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Liu XC,Lemma-Gray Pdoi
10.1002/bit.1144subject
Has Abstractpub_date
2001-09-20 00:00:00pages
524-7issue
6eissn
0006-3592issn
1097-0290pii
10.1002/bit.1144journal_volume
74pub_type
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