Enzymatic synthesis of unsaturated fatty acid glucoside esters for dermo-cosmetic applications.

abstract：:This article describes a method for the chemical immobilization of concanavalin A (Con A) on the inside wall of a single hollow cellulose fiber for use in glucose affinity sensor. Periodate oxidation of cellulose fiber followed by a spacer for Con A attachment was deemed to be the most optimal procedure for achieving ...

journal_title：Biotechnology and bioengineering

authors： Srinivasan KR,Mansouri S,Schultz JS

更新日期：1986-02-01 00:00:00

abstract：:In this work the question was addressed if in nitrite-oxidizing activated sludge systems the environmental competition between Nitrobacter spp. and Nitrospira spp., which only recently has been discovered to play a role in these systems, is affected by the nitrite concentrations. Two parallel chemostats were inoculate...

journal_title：Biotechnology and bioengineering

authors： Nogueira R,Melo LF

更新日期：2006-09-05 00:00:00

abstract：:Cobalt chromium (CoCr) alloy is widely used in orthopedic implants but its functional longevity is susceptible to inflammation related complications. Reduction of the development of chronic inflammation on the biomaterial surface would enhance direct bone-implant bonding and improve implant survival and long-term resu...

journal_title：Biotechnology and bioengineering

authors： Tan HC,Poh CK,Cai Y,Soe MT,Wang W

更新日期：2013-03-01 00:00:00

abstract：:Cellulolytic fungi, such as Trichoderma reesei, T. lignorum, and Chaetomium cellulolyticum reach a low packing density of mycelia when grown on straw under conditions of solid substrate fermentation. The low packing density is shown to be caused partially by the geometric limitation of the growth of mycelia in the sub...

journal_title：Biotechnology and bioengineering

authors： Laukevics JJ,Apsite AF,Viesturs US,Tengerdy RP

更新日期：1985-12-01 00:00:00

abstract：:Batch hydrolysis kinetics of paper birch (Betula papyrifera) xylan and its associated acetyl groups in dilute sulfuric acid have been measured for acid concentrations of between 0.04 and 0.18M and temperatures of between 100 and 170 degrees C. Only 5% of the cellulose was hydrolyzed for up to 85% xylan removal. Rate d...

journal_title：Biotechnology and bioengineering

authors： Maloney MT,Chapman TW,Baker AJ

更新日期：1985-03-01 00:00:00

abstract：:The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...

journal_title：Biotechnology and bioengineering

authors： Prokop A,Ratcliffe HD,Fatayer MI,Al-Awadhi N,Khamis A,Murad M,Bond C,Hamdan IY

更新日期：1984-09-01 00:00:00

abstract：:James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Khosla C

更新日期：2002-09-05 00:00:00

abstract：:The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of...

journal_title：Biotechnology and bioengineering

authors： Dols-Lafargue M,Willemot RM,Monsan PF,Remaud-Simeon M

更新日期：2001-11-05 00:00:00

abstract：:Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

journal_title：Biotechnology and bioengineering

authors： Miyasaka Y,Rha C,Sinskey AJ

更新日期：1980-10-01 00:00:00

abstract：:Polymers produced by horseradish-peroxidase-catalyzed coupling of phenols have been explored as potential substitutes for phenol-formaldehyde resins. To overcome low substrate solubilities and product molecular weights in water, enzymatic polymerizations in aqueous-organic mixtures have been examined. Peroxidase vigor...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Marletta MA,Klibanov AM

更新日期：1987-07-01 00:00:00

abstract：:Integrated process concepts for enzymatic cephalexin synthesis were investigated by our group, and this article focuses on the integration of reactions and product removal during the reactions. The last step in cephalexin production is the enzymatic kinetic coupling of activated phenylglycine (phenylglycine amide or p...

journal_title：Biotechnology and bioengineering

authors： Schroën CG,Nierstrasz VA,Bosma R,Kroon PJ,Tjeerdsma PS,DeVroom E,VanderLaan JM,Moody HM,Beeftink HH,Janssen AE,Tramper J

更新日期：2002-10-20 00:00:00

abstract：:The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and rem...

journal_title：Biotechnology and bioengineering

authors： Evans LL,Burns MA

更新日期：1995-12-05 00:00:00

abstract：:High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation in this compartment. Periplasmic heat-sh...

journal_title：Biotechnology and bioengineering

authors： Wu MS,Pan KL,Chou CP

更新日期：2007-04-01 00:00:00

abstract：:A spinning cylindrical filter is often used to retain mammalian cells in a continuous perfusion bioreactor. This device, known as a spinfilter, has typically been with pore size smaller than the cell particles (single cells or aggregates) in order to achieve cell separation. For single cells in suspension, such an ope...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1992-10-20 00:00:00

abstract：:Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition, and function of the native enthesis. In the ligament-to-bone interface, this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradati...

journal_title：Biotechnology and bioengineering

authors： Harris E,Liu Y,Cunniffe G,Morrissey D,Carroll S,Mulhall K,Kelly DJ

更新日期：2017-10-01 00:00:00

abstract：:A detailed mechanistic model of enzymatic cellulose hydrolysis has been developed. The behavior of individual cellulase enzymes and parameters describing the cellulose surface properties are included. Results obtained for individual enzymes (T. reesei endoglucanase 2 and cellobiohydrolase I) and systems with both enzy...

journal_title：Biotechnology and bioengineering

authors： Levine SE,Fox JM,Blanch HW,Clark DS

更新日期：2010-09-01 00:00:00

abstract：:The inhibition of the maximum specific growth and fermentation rate of Zymomonas mobilis by ethanol was studied in turbidostat cultures at constant and stepwise changed ethanol concentrations. Up to 50 g/L ethanol, the inhibition kinetics can be approximated by a linear relationship between the specific growth rate an...

journal_title：Biotechnology and bioengineering

authors： Jöbses IM,Roels JA

更新日期：1986-04-01 00:00:00

abstract：:It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

journal_title：Biotechnology and bioengineering

authors： Xia F,Nagrath D,Garde S,Cramer SM

更新日期：2004-08-05 00:00:00

abstract：:To build complex genetic networks with predictable behaviors, synthetic biologists use libraries of modular parts that can be characterized in isolation and assembled together to create programmable higher-order functions. Characterization experiments and computational models for gene regulatory parts operating in iso...

journal_title：Biotechnology and bioengineering

authors： Westbrook A,Tang X,Marshall R,Maxwell CS,Chappell J,Agrawal DK,Dunlop MJ,Noireaux V,Beisel CL,Lucks J,Franco E

更新日期：2019-05-01 00:00:00

abstract：:Steam explosion after sulphur dioxide impregnation of wood chips is an effective method for improving the enzymatic digestibility of cellulose in the softwood Pinus radiata. Digestibility of pretreated fiber was progressively increased by altering the conditions of steam explosion. With increasing digestibility, there...

journal_title：Biotechnology and bioengineering

authors： Wong KK,Deverell KF,Mackie KL,Clark TA,Donaldson LA

更新日期：1988-04-05 00:00:00

abstract：:Molecular chaperones in aqueous-organic mixtures can broaden the utility of biocatalysis by stabilizing enzymes in denaturing conditions. We have designed a self-renaturing enzyme-chaperone chimera consisting of penicillin amidase and a thermophilic chaperonin that functions in aqueous-organic mixtures. The flexible l...

journal_title：Biotechnology and bioengineering

authors： Bergeron LM,Gomez L,Whitehead TA,Clark DS

更新日期：2009-04-01 00:00:00

abstract：:An integrated solvent (ABE) fermentation and product removal process was investigated. A stable solvent productivity of 3.5 g/L h was achieved by using cells of Clostridium acetobutylicum immobilized onto a packed bed of bonechar, coupled with continuous product removal by pervaporation. Using a concentrated feed solu...

journal_title：Biotechnology and bioengineering

authors： Friedl A,Qureshi N,Maddox IS

更新日期：1991-08-20 00:00:00

abstract：:The emissions of nitrous oxide (N(2)O) and nitric oxide (NO) from biological nitrogen removal (BNR) operations via nitrification and denitrification is gaining increased prominence. While many factors relevant to the operation of denitrifying reactors can influence N(2)O and NO emissions from them, the role of differe...

journal_title：Biotechnology and bioengineering

authors： Lu H,Chandran K

更新日期：2010-06-15 00:00:00

abstract：:In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unkno...

journal_title：Biotechnology and bioengineering

authors： Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC

更新日期：2020-01-01 00:00:00

abstract：:Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Approximately 8 of 14 lysines per trypsin molecule were modified. This derivative (EG trypsin) was more stable than native between 30 degrees and 70 degrees C: T50 values were 59 degrees C and 46 degrees C, respec...

journal_title：Biotechnology and bioengineering

authors： Murphy A,O Fágáin C

更新日期：1998-05-20 00:00:00

abstract：:Whole cell pyruvate decarboxylase (PDC) from Candida utilis enhanced the enzymatic production of (R)-phenylacetylcarbinol (PAC) in an aqueous/octanol biotransformation compared to the partially purified PDC especially for a lower range of initial activities (0.3-2.5 U/mL). With an initial activity of 1.1 U/mL and at a...

journal_title：Biotechnology and bioengineering

authors： Satianegara G,Rogers PL,Rosche B

更新日期：2006-08-20 00:00:00

abstract：:Genetically transformed shooty teratomas of Atropa belladonna and a Duboisia leichhardtii x D. myoporoides hybrid were studied for biotransformation of tropane alkaloids in shake flasks and bioreactors. Although de novo synthesis of hyoscyamine and scopolamine was limited, shoots of both species were able to transloca...

journal_title：Biotechnology and bioengineering

authors： Subroto MA,Kwok KH,Hamill JD,Doran PM

更新日期：1996-03-05 00:00:00

abstract：:Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of the succinate of the desig...

journal_title：Biotechnology and bioengineering

authors： Tokuyama K,Toya Y,Horinouchi T,Furusawa C,Matsuda F,Shimizu H

更新日期：2018-06-01 00:00:00

abstract：:The present study evaluated the influence of water activity and lactose concentration on the synthesis of galactooligosaccharides (GOS), by means of a hyperthermophilic beta-glycosidase in an organic system. The production of GOS gradually grew as water activity increased in the reaction system; later, their synthesis...

journal_title：Biotechnology and bioengineering

authors： Cruz-Guerrero AE,Gómez-Ruiz L,Viniegra-González G,Bárzana E,García-Garibay M

更新日期：2006-04-20 00:00:00

abstract：:The results of an initial study of enzymatic catalysis in metastable supersaturated solutions of carbohydrates are presented. It has been shown that such solutions, formed in the presence of small amounts of water and alcohol as plasticizers, are sufficiently stable under ambient conditions to enable enzymatic transfo...

journal_title：Biotechnology and bioengineering

authors： Millqvist-Fureby A,Gill IS,Vulfson EN

更新日期：1998-10-20 00:00:00