Abstract:
:We have used a high-throughput LC/MS/UV/ELSD method to rapidly determine the absolute quantity and purity of 42 organic compounds from seven lead discovery libraries. A general calibration curve generated from a different set of 42 compounds with seven different scaffolds was used in this analysis. We have also studied 33 organic compounds with different molecular weight (MW) by LC/MS/UV/ELSD to investigate the effect of MW on ELSD response and the accuracy for purity and quantity measurement using UV(214) and ELSD. A general ELSD calibration curve from these compounds was also generated to quantify 42 library compounds. Purity measurement by ELSD underestimates the amounts of impurities due to a reduced ELSD response from smaller molecular weight impurities often produced in library synthesis. Absolute quantity determination by ELSD is more accurate (RSD 28%) than that by UV(214) (48%) using a calibration curve generated from the same set of compounds with diverse MWs. Error assessment for the measurement of absolute quantity of a class of commercial compounds and a class of representing reference compounds from seven diverse lead discovery libraries shows that structurally related compounds should be used to generate calibration curves to sustain smaller deviation.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Fang L,Pan J,Yan Bdoi
10.1002/1097-0290(2000)71:2<162::aid-bit1006>3.0.csubject
Has Abstractpub_date
2000-01-01 00:00:00pages
162-71issue
2eissn
0006-3592issn
1097-0290pii
10.1002/1097-0290(2000)71:2<162::AID-BIT1006>3.0.Cjournal_volume
71pub_type
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