Immobilized phenylalanine hydroxylase through the SH groups.

abstract：:We have produced Bioglass coatings for Orthopedic implants by using a novel coating technique, CoBlast. The two resultant surfaces, designated BG and hydroxyapatite (HA)/BG, were compared with their HA counterpart, OsteoZip in terms of osteoblastic cell attachment, adhesion, proliferation, differentiation, and growth ...

journal_title：Biotechnology and bioengineering

authors： Tan F,Naciri M,Al-Rubeai M

更新日期：2011-02-01 00:00:00

abstract：:The whole cell tyrosine phenol-lyase (TPL, E.C. 4.1.99.2) activity of Erwinia herbicola (ATCC 21434) was microen-capsulated. We studied the use of this for the conversion of ammonia and pyruvate along with phenol or catechol, respectively, into L-tyrosine or dihydroxyphenyl-L-alanine (L-dopa). The reactions are releva...

journal_title：Biotechnology and bioengineering

authors： Lloyd-George I,Chang TM

更新日期：1995-12-20 00:00:00

abstract：:The reconstruction of functioning human tissues ex vivo is becoming an important part of biotechnology. There are compelling scientific, clinical, and biotechnological reasons for fully or partially reconstituting human tissues such as skin, bone marrow, and liver ex vivo. In particular, bone marrow is a tissue of muc...

journal_title：Biotechnology and bioengineering

authors： Koller MR,Palsson BO

更新日期：1993-10-01 00:00:00

abstract：:A perfusion culture system was developed to investigate the oxygenation of high-density hybridoma cell cultures. The culture system was composed of a stirred-tank bioreactor and an external microfiltration hollow fiber cartridge for medium perfusion. Cell growth and antibody production were examined with large bubble ...

journal_title：Biotechnology and bioengineering

authors： Zhang S,Handa-Corrigan A,Spier RE

更新日期：1993-03-25 00:00:00

abstract：:The DeltaSf of one unit carbon formula weight of Escherichia coli K-12 cells, when grown on succinic acid, was calculated to be -80.13 J/deg. This value could then be used to calculate the entropy change accompanying the anabolism and metabolism of succinic acid to be 30.82 J/deg and 32.40 J/mol deg, respectively. The...

journal_title：Biotechnology and bioengineering

authors： Battley EH

更新日期：1993-02-20 00:00:00

abstract：:This article provides the analysis on biomass yields and energetic yields of the oleaginous yeasts. The biomass yields of the oleaginous yeasts are consistently lower than nonoleaginous microorganisms, whereas their energetic yields are higher. Data inconsistencies of literature are explained by the variation of energ...

journal_title：Biotechnology and bioengineering

authors： Pan JG,Rhee JS

更新日期：1986-01-01 00:00:00

abstract：:Capitalizing on components evolved to metabolize ethanol in Aspergillus nidulans, we previously designed the first molecular gas-gene expression interface using gaseous acetaldehyde as the major inducer. This fungus-derived acetaldehyde-inducible gene regulation (AIR) system operated perfectly and enabled precise and ...

journal_title：Biotechnology and bioengineering

authors： Weber W,Spielmann M,Daoud El-Baba M,Keller B,Aubel D,Fussenegger M

更新日期：2005-06-30 00:00:00

abstract：:Batch fermentations of glucose to ethanol by Z. Mobilis.(ATCC 10988) were examined in several semidefined nutrient media. The measurement of acid produced by the microorganism was used to study its transient fermentation characteristics. Limitation of nitrogen source in the semidefined medium of Rogers and coworkers(2...

journal_title：Biotechnology and bioengineering

authors： Veeramallu U,Agrawal P

更新日期：1988-05-20 00:00:00

abstract：:Galactose oxidase (E.C. 1.1.3.9) was covalently immobilized to chemically modified porous silica particles by reaction of the native enzyme with pendant benzoyl azide groups on the carrier. The enzyme loading on the carrier was 100-150 units per milliliter. The immobilized enzyme was incorporated into a hardware assem...

journal_title：Biotechnology and bioengineering

authors： Dahodwala SK,Weibel MK,Humphrey AE

更新日期：1976-12-01 00:00:00

abstract：:A spinning cylindrical filter is often used to retain mammalian cells in a continuous perfusion bioreactor. This device, known as a spinfilter, has typically been with pore size smaller than the cell particles (single cells or aggregates) in order to achieve cell separation. For single cells in suspension, such an ope...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1992-10-20 00:00:00

abstract：:10-Deacetylabaccatin III (10 DAB), an important precursor for paclitaxel semisynthesis, is enhanced in yew extracts using C10-deacetylase and C13-deacylase enzymes.(4) C10-deacetylase is an intracellular enzyme produced by the fermentation of a soil microorganism, Nocardioides luteus (SC 13912). During the fermentatio...

journal_title：Biotechnology and bioengineering

authors： Nanduri VB,Hanson RL,Laporte TL,Ko RY,Patel RN,Szarka LJ

更新日期：1995-12-05 00:00:00

abstract：:We have developed a simple and robust transient expression system utilizing the 25 kDa branched cationic polymer polyethylenimine (PEI) as a vehicle to deliver plasmid DNA into suspension-adapted Chinese hamster ovary cells synchronized in G2/M phase of the cell cycle by anti-mitotic microtubule disrupting agents. The...

journal_title：Biotechnology and bioengineering

authors： Tait AS,Brown CJ,Galbraith DJ,Hines MJ,Hoare M,Birch JR,James DC

更新日期：2004-12-20 00:00:00

abstract：:The insect larvae/baculovirus protein production process was dramatically simplified by expressing fusion proteins containing green fluorescent protein (GFP) and the product-of-interest. In this case, human interleukin-2 (hIL-2) and chloramphenicol acetyl-transferase (CAT) were model products. Specifically, our fusion...

journal_title：Biotechnology and bioengineering

authors： Cha HJ,Dalal NG,Pham MQ,Vakharia VN,Rao G,Bentley WE

更新日期：1999-11-05 00:00:00

abstract：:Human skin-derived precursor cells (hSKPs) are multipotent adult stem cells found in the dermis of human skin. Incorporation of hSKPs into split-thickness skin grafts (STSGs), the current gold standard to treat severe burns or tissue resections, has been proposed as a treatment option to enhance skin wound healing and...

journal_title：Biotechnology and bioengineering

authors： Surrao DC,Boon K,Borys B,Sinha S,Kumar R,Biernaskie J,Kallos MS

更新日期：2016-12-01 00:00:00

abstract：:A kinetic model describing the cephalosporin biosynthesis in Streptomyces clavuligerus was developed. Using previously reported kinetic data of biosynthetic enzymes, we examined the kinetics of cephalosporin production. The predicted time profile of the specific production rate during a batch culture parallels that of...

journal_title：Biotechnology and bioengineering

authors： Malmberg LH,Hu WS

更新日期：1991-10-20 00:00:00

abstract：:A two-dimensional model for quantitative evaluation of the effect of convective and diffusive substrate transport on biofilm heterogeneity was developed. The model includes flow computation around the irregular biofilm surface, substrate mass transfer by convection and diffusion, biomass growth, and biomass spreading....

journal_title：Biotechnology and bioengineering

authors： Picioreanu C,Van Loosdrecht MC,Heijnen JJ

更新日期：2000-09-05 00:00:00

abstract：:Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time course studies the rea...

journal_title：Biotechnology and bioengineering

authors： Medve J,Karlsson J,Lee D,Tjerneld F

更新日期：1998-09-05 00:00:00

abstract：:CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...

journal_title：Biotechnology and bioengineering

authors： deZengotita VM,Schmelzer AE,Miller WM

更新日期：2002-02-15 00:00:00

abstract：:Melanoma is now the fifth most common type of cancer in North America. At present, there is no optimal treatment for this cancer. However, the lowering of the tyrosine level can inhibit the growth of melanoma. Unfortunately, this diet restriction cannot be humanly tolerated and causes vomiting, nausea, and severe body...

journal_title：Biotechnology and bioengineering

authors： Yu B,Chang TM

更新日期：2004-06-30 00:00:00

abstract：:Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...

journal_title：Biotechnology and bioengineering

authors： Bohle K,Ross A

更新日期：2011-09-01 00:00:00

abstract：:Intracellular metabolite concentration and enzyme activity measurements were made to explain the new metabolic and growth phenomena seen in the micro-aerobic, continuous yeast cultures described in Part I. The results of these assays suggested mechanisms for the observed maximum in the specific ethanol productivity as...

journal_title：Biotechnology and bioengineering

authors： Grosz R,Stephanopoulos G

更新日期：1990-12-05 00:00:00

abstract：:The high commercial values of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have driven a strain-improvement program, aimed at increasing the content of those fatty acids in the microalga Pavlova lutheri (SMBA 60) as parent strain. After a round of mutation using UV-light as mutagenic agent, an isolate strain...

journal_title：Biotechnology and bioengineering

authors： Meireles LA,Guedes AC,Malcata FX

更新日期：2003-01-05 00:00:00

abstract：:Phosphite dehydrogenase represents a new enzymatic system for regenerating reduced nicotinamide cofactors for industrial biocatalysis. We previously engineered a variant of phosphite dehydrogenase with relaxed cofactor specificity and significantly increased activity and stability. Here we performed one round of rando...

journal_title：Biotechnology and bioengineering

authors： McLachlan MJ,Johannes TW,Zhao H

更新日期：2008-02-01 00:00:00

abstract：:The adsorption of Thiobacillus ferrooxidans to coal surfaces has been studied. Adsorption experiments were conducted on coal samples from eight different Eastern coal fields. In all cases the adsorption process was at least 90% complete within the first two minutes following inoculation. The results of these experimen...

journal_title：Biotechnology and bioengineering

authors： Bagdigian RM,Myerson AS

更新日期：1986-04-01 00:00:00

abstract：:Cost-effective production of biopharmaceuticals on a large scale can be carried out by perfusion cultures of mammalian cells. One problem with this mode of operation for submerged free-cell cultures is the requirement for an efficient cell separation device located in the effluent stream. The present work investigates...

journal_title：Biotechnology and bioengineering

authors： Docoslis A,Kalogerakis N,Behie LA,Kaler KV

更新日期：1997-05-05 00:00:00

abstract：:The inhibition of the maximum specific growth and fermentation rate of Zymomonas mobilis by ethanol was studied in turbidostat cultures at constant and stepwise changed ethanol concentrations. Up to 50 g/L ethanol, the inhibition kinetics can be approximated by a linear relationship between the specific growth rate an...

journal_title：Biotechnology and bioengineering

authors： Jöbses IM,Roels JA

更新日期：1986-04-01 00:00:00

abstract：:Two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguishable by differences in their surface topography. Immobilizations were per...

journal_title：Biotechnology and bioengineering

authors： Wasserman BP,Hultin HO,Jacobson BS

更新日期：1980-02-01 00:00:00

abstract：:Addition of Tween 85 to aqueous suspensions of Anabaena variabilis induced photosynthetic evolution of hydrogen over a time span of several weeks: As much as 148 nmol H(2)/h . mg dry weight was produced in the first week by a suspension containing 4.2 mg dry weight of cells and 77 mM Tween 85. The chemical structure o...

journal_title：Biotechnology and bioengineering

authors： Famiglietti M,Hochkoeppler A,Luisi PL

更新日期：1993-10-01 00:00:00

abstract：:A recombinant strain of Salmonella enterica serovar Typhimurium (mutant in propionate-activation activity) was metabolically engineered to control the composition of poly(3-hydroxybutyrate-co-3-hydroxy- valerate) (PHBV), a polyhydroxyalkanoate copolymer with commercially desirable properties. A gene (prpE) encoding pr...

journal_title：Biotechnology and bioengineering

authors： Aldor I,Keasling JD

更新日期：2001-09-01 00:00:00

abstract：:Retroviral-derived biopharmaceuticals (RV) target numerous therapeutic applications, from gene therapy to virus-like particle (rVLP)-based vaccines. During particle formation, beside the pseudotyped envelope proteins, RV can incorporate proteins derived from the virus producer cells (VPC). This may be detrimental by r...

journal_title：Biotechnology and bioengineering

authors： Rodrigues AF,Guerreiro MR,Santiago VM,Dalba C,Klatzmann D,Alves PM,Carrondo MJ,Coroadinha AS

更新日期：2011-11-01 00:00:00