Calculation of entropy change accompanying growth of Escherichia coli K-12 on succinic acid.

abstract：:With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin d...

journal_title：Biotechnology and bioengineering

authors： Najjar M,Manzoli V,Abreu M,Villa C,Martino MM,Molano RD,Torrente Y,Pileggi A,Inverardi L,Ricordi C,Hubbell JA,Tomei AA

更新日期：2015-09-01 00:00:00

abstract：:We have developed a new cell surface display system using a major outer membrane protein of Pseudomonas aeruginosa OprF as an anchoring motif. Pseudomonas fluorescens SIK W1 lipase gene was fused to the truncated oprF gene by C-terminal deletion fusion strategy. The truncated OprF-lipase fusion protein was successfull...

journal_title：Biotechnology and bioengineering

authors： Lee SH,Choi JI,Han MJ,Choi JH,Lee SY

更新日期：2005-04-20 00:00:00

abstract：:In vitro engineering systems can be powerful tools for studying tissue development in response to biophysical stimuli as well as for evaluating the functionality of engineered tissue grafts. It has been challenging, however, to develop systems that adequately integrate the application of biomimetic mechanical strain t...

journal_title：Biotechnology and bioengineering

authors： Cook CA,Huri PY,Ginn BP,Gilbert-Honick J,Somers SM,Temple JP,Mao HQ,Grayson WL

更新日期：2016-08-01 00:00:00

abstract：:Expression of recombinant proteins in Escherichia coli often results in the formation of insoluble inclusion bodies, In case of expression of eukaryotic proteins containing cysteine, which may form disulfide bonds in the native active protein, often nonnative inter- and intramolecular disulfide bonds exist in the incl...

journal_title：Biotechnology and bioengineering

authors： Fischer B,Sumner I,Goodenough P

更新日期：1993-01-05 00:00:00

abstract：:Small interfering RNAs (siRNAs) are invaluable research tools for studying gene functions in mammalian cells. siRNAs are mainly produced by chemical synthesis or by enzymatic digestion of double-stranded RNA (dsRNA) produced in vitro. Recently, bacterial cells, engineered with ectopic plant viral siRNA binding protein...

journal_title：Biotechnology and bioengineering

authors： Kaur G,Cheung HC,Xu W,Wong JV,Chan FF,Li Y,McReynolds L,Huang L

更新日期：2018-09-01 00:00:00

abstract：:A conceptual model describing the relative roles of sorption and precipitation processes for metals in solid-solution suspensions is presented. The model performance is demonstrated using experimental data on sorption and precipitation of metals in samples of activated sludge mixed liquor. Based on the experimental re...

journal_title：Biotechnology and bioengineering

authors： Kodukula PS,Patterson JW,Surampalli RY

更新日期：1994-04-15 00:00:00

abstract：:This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...

journal_title：Biotechnology and bioengineering

authors： Saucedo VM,Karim MN

更新日期：1997-07-20 00:00:00

abstract：:Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusi...

journal_title：Biotechnology and bioengineering

authors： Wayman FM,Mattey M

更新日期：2000-02-20 00:00:00

abstract：:In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

journal_title：Biotechnology and bioengineering

authors： van der Pol L,Bakker WA,Tramper J

更新日期：1992-06-05 00:00:00

abstract：:The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

journal_title：Biotechnology and bioengineering

authors： Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

更新日期：2006-08-20 00:00:00

abstract：:Shikimic acid is one of several industrially interesting chiral starting materials formed in the aromatic amino acid pathway of plants and microorganisms. In this study, the physiology of a shikimic acid producing strain of Escherichia coli (derived from W3110) deleted in aroL (shikimic acid kinase II gene), was compa...

journal_title：Biotechnology and bioengineering

authors： Johansson L,Lindskog A,Silfversparre G,Cimander C,Nielsen KF,Lidén G

更新日期：2005-12-05 00:00:00

abstract：:A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...

journal_title：Biotechnology and bioengineering

authors： Sadana A,Henley JP

更新日期：1987-10-20 00:00:00

abstract：:Neural stem cells (NSCs) are undifferentiated, primitive cells with important potential applications including the replacement of neural tissue lost due to neurodegenerative diseases, including Parkinson's disease, as well as brain and spinal cord injuries, including stroke. We have developed methods to rapidly expand...

journal_title：Biotechnology and bioengineering

authors： Alam S,Sen A,Behie LA,Kallos MS

更新日期：2004-11-05 00:00:00

abstract：:The second messenger cyclic dimeric (3'→5') GMP (cyclic di-GMP or c-di-GMP) has been implicated in the transition between motile and sessile lifestyles in bacteria. In this study, we demonstrate that biofilm formation, cellular aggregation or flocculation, and cellular buoyancy are under the control of c-di-GMP in Syn...

journal_title：Biotechnology and bioengineering

authors： Agostoni M,Waters CM,Montgomery BL

更新日期：2016-02-01 00:00:00

abstract：:To build complex genetic networks with predictable behaviors, synthetic biologists use libraries of modular parts that can be characterized in isolation and assembled together to create programmable higher-order functions. Characterization experiments and computational models for gene regulatory parts operating in iso...

journal_title：Biotechnology and bioengineering

authors： Westbrook A,Tang X,Marshall R,Maxwell CS,Chappell J,Agrawal DK,Dunlop MJ,Noireaux V,Beisel CL,Lucks J,Franco E

更新日期：2019-05-01 00:00:00

abstract：:The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5-norbornene-2.3-dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with ...

journal_title：Biotechnology and bioengineering

authors： Büttner W,Becker M,Rupprich C,Boeden HF,Henklein P,Loth F,Dautzenberg H

更新日期：1989-01-05 00:00:00

abstract：:The growth and product formation of a Savinase-producing Bacillus clausii were investigated in high-cell-density fed-batch cultivations with both linear and exponential feed profiles. The highest specific productivity of Savinase was observed shortly after the end of the initial batch phase for all feed profiles appli...

journal_title：Biotechnology and bioengineering

authors： Christiansen T,Michaelsen S,Wümpelmann M,Nielsen J

更新日期：2003-08-05 00:00:00

abstract：:A uniquely simple model is developed to describe the NO(3) (-) and NO(2) (-) concentration profiles within a dentrification fluidized bed biological reactor. This simple model is compared to experimental data, and to a more complex model similar to those previously proposed in the literature. The simple model fits the...

journal_title：Biotechnology and bioengineering

authors： Brosilow BJ,Schnitzer M,Tarre S,Green M

更新日期：1997-06-20 00:00:00

abstract：:Agility to schedule and execute cell culture manufacturing campaigns quickly in a multi-product facility will play a key role in meeting the growing demand for therapeutic proteins. In an effort to shorten campaign timelines, maximize plant flexibility and resource utilization, we investigated the initiation of cell c...

journal_title：Biotechnology and bioengineering

authors： Seth G,Hamilton RW,Stapp TR,Zheng L,Meier A,Petty K,Leung S,Chary S

更新日期：2013-05-01 00:00:00

abstract：:Oral delivery of insulin to diabetic patients is highly desirable because it would be non-invasive and more closely mimic normal physiology, but this route of administration typically results in low bioavailability due to low pH and enzymatic degradation along the gastrointestinal tract. To explore an alternative appr...

journal_title：Biotechnology and bioengineering

authors： Ng DT,Sarkar CA

更新日期：2011-08-01 00:00:00

abstract：:The activities of lipase from Candida cylindracea and Rhizopus delemar have been investigated in water/AOT/iso-octane reverse micellar media through the use of two esterification reactions: fatty acid-alcohol esterification and glyceride synthesis. Such media promotes the occurrence of these two lipase-catalyzed react...

journal_title：Biotechnology and bioengineering

authors： Hayes DG,Gulari E

更新日期：1990-04-05 00:00:00

abstract：:A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

journal_title：Biotechnology and bioengineering

authors： Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

更新日期：2016-04-01 00:00:00

abstract：:Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...

journal_title：Biotechnology and bioengineering

authors： Bohle K,Ross A

更新日期：2011-09-01 00:00:00

abstract：:Plant cell walls are composed primarily of cellulose, hemicelluloses, lignins, and pectins. Of these components, lignins exhibit unique chemistry and physiological functions. Although lignins can be used as a product feedstock or as a fuel, lignins are also generally seen as a barrier to efficient enzymatic breakdown ...

journal_title：Biotechnology and bioengineering

authors： Donohoe BS,Decker SR,Tucker MP,Himmel ME,Vinzant TB

更新日期：2008-12-01 00:00:00

abstract：:A facile methodology has been developed for covalently derivatizing the surfaces of common materials with a designed antibacterial polycation, poly(vinyl-N-pyridinium bromide), wherein the first, key step involves surface coating with a nanolayer of silica. Various commercial synthetic polymers derivatized in this man...

journal_title：Biotechnology and bioengineering

authors： Tiller JC,Lee SB,Lewis K,Klibanov AM

更新日期：2002-08-20 00:00:00

abstract：:Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern...

journal_title：Biotechnology and bioengineering

authors： Yigit S,Dinjaski N,Kaplan DL

更新日期：2016-05-01 00:00:00

abstract：:Two general models for batch simultaneous enzymatic and microbial reaction (SEMR) processes are presented, the second derived from and simpler than the first and accounting for enzyme denaturation. Using the second model and parameter values from the literature, simulation was used to examine a range of enzyme additio...

journal_title：Biotechnology and bioengineering

authors： Asenjo JA,Sun WH,Spencer JL

更新日期：1991-05-01 00:00:00

abstract：:Formaldehyde is a prevalent environmental toxin and a key intermediate in single carbon metabolism. The ability to monitor formaldehyde concentration is, therefore, of interest for both environmental monitoring and for metabolic engineering of native and synthetic methylotrophs, but current methods suffer from low sen...

journal_title：Biotechnology and bioengineering

authors： Woolston BM,Roth T,Kohale I,Liu DR,Stephanopoulos G

更新日期：2018-01-01 00:00:00

abstract：:To widen the biological control function of a genetically modified Bacillus thuringiensis subsp leesis strain BMB-005, an acyl homoserine lactonase (AHL lactonase) gene aiiA transcribed by the promoter of insecticidal crystal protein coding gene cry3A, was transformed into strain BMB-005. The amount of AHL lactonase p...

journal_title：Biotechnology and bioengineering

authors： Zhu C,Yu Z,Sun M

更新日期：2006-10-20 00:00:00

abstract：:The results of an initial study of enzymatic catalysis in metastable supersaturated solutions of carbohydrates are presented. It has been shown that such solutions, formed in the presence of small amounts of water and alcohol as plasticizers, are sufficiently stable under ambient conditions to enable enzymatic transfo...

journal_title：Biotechnology and bioengineering

authors： Millqvist-Fureby A,Gill IS,Vulfson EN

更新日期：1998-10-20 00:00:00