Abstract:
:The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. The reporter bacterium, HK44, harbors a reporter plasmid, pUTK21, which contains a transcriptional fusion between the nahG gene in the lower pathway of the catabolic plasmic NAH7 and a luxCDABE gene cassette. The upper nah pathway and the lux pathway in pUTK21 are induced by salicylate. The lux enzymes catalyze the light reaction. HK44 demonstrated a quantitative relationship between salicylate concentration and degradation. Light intensity mimicked salicylate concentration, whereas degradation was first order in biomass and first order in salicylate concentration, with a degradation constant of 2.23 x 10(-2) dm(3) g(-1) min(-1).
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GSdoi
10.1002/(SICI)1097-0290(19970605)54:5<491::AID-BITsubject
Has Abstractpub_date
1997-06-05 00:00:00pages
491-502issue
5eissn
0006-3592issn
1097-0290journal_volume
54pub_type
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