Abstract:
:Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum pH and temperature were determined to be 5.5 and 55 degrees C, respectively. The stability of IC against change in the pH and temperature was improved by the immobilization. Solvolysis of 3N-NaOH-treated cellulose, with IC under the optimum conditions found here, led to the production of low-molecular-weight compounds.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Takeuchi T,Makino Kdoi
10.1002/bit.260290203subject
Has Abstractpub_date
1987-02-01 00:00:00pages
160-4issue
2eissn
0006-3592issn
1097-0290journal_volume
29pub_type
杂志文章abstract::Fast and efficient production of recombinant proteins for structural and functional studies is a crucial issue for research and for industry. To this end, we have developed an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant prot...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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更新日期:1984-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/bit.260280609
更新日期:1986-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-05-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:1987-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-08-01 00:00:00
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更新日期:2012-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.260460309
更新日期:1995-05-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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更新日期:1998-04-05 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2016-05-01 00:00:00
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更新日期:2015-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2001-02-20 00:00:00
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更新日期:2003-03-05 00:00:00
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pub_type: 杂志文章
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更新日期:1993-04-05 00:00:00