A comparison of two empirical models for the enzymatic hydrolysis of pretreated poplar wood.

abstract：:A semimechanistic multi-reaction kinetic model was developed to describe the enzymatic hydrolysis of a lignocellulosic biomass, creeping wild ryegrass (CWR; Leymus triticoides). This model incorporated one homogeneous reaction of cellobiose-to-glucose and two heterogeneous reactions of cellulose-to-cellobiose and cell...

journal_title：Biotechnology and bioengineering

authors： Zheng Y,Pan Z,Zhang R,Jenkins BM

更新日期：2009-04-15 00:00:00

abstract：:A model for cometabolism is verified experimentally for a defined methanotrophic mixed culture. The model includes the effects of cell growth, endogenous cell decay, product toxicity, and competitive inhibition with the assumption that cometabolic transformation rates are enhanced by reducing power obtained from oxida...

journal_title：Biotechnology and bioengineering

authors： Chang WK,Criddle CS

更新日期：1997-12-05 00:00:00

abstract：:Screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that Coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. Treatment of the same effluent with this fungus, immobilized in beads...

journal_title：Biotechnology and bioengineering

authors： Livernoche D,Jurasek L,Desrochers M,Dorica J,Veliky IA

更新日期：1983-08-01 00:00:00

abstract：:Advanced cell and gene therapies such as chimeric antigen receptor T-cell immunotherapies (CAR-T), present a novel therapeutic modality for the treatment of acute and chronic conditions including acute lymphoblastic leukemia and non-Hodgkin lymphoma. However, the development of such immunotherapies requires the manufa...

journal_title：Biotechnology and bioengineering

authors： Costariol E,Rotondi M,Amini A,Hewitt CJ,Nienow AW,Heathman TRJ,Micheletti M,Rafiq QA

更新日期：2019-10-01 00:00:00

abstract：:Mathematical models to describe transcription (Arnold et al. (2001); Biotech Bioeng 72:548-561) and translation (Mehra et al. (2003); Biotech Bioeng 84:822-841) in bacteria are modified in order to improve reaction kinetics and to include the number of polymerase molecules that are active on the DNA, as well as to inc...

journal_title：Biotechnology and bioengineering

authors： Kremling A

更新日期：2007-03-01 00:00:00

abstract：:Rifamycin B is an important polyketide antibiotic used in the treatment of tuberculosis and leprosy. We present results on medium optimization for Rifamycin B production via a barbital insensitive mutant strain of Amycolatopsis mediterranei S699. Machine-learning approaches such as Genetic algorithm (GA), Neighborhood...

journal_title：Biotechnology and bioengineering

authors： Bapat PM,Wangikar PP

更新日期：2004-04-20 00:00:00

abstract：:A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, ...

journal_title：Biotechnology and bioengineering

authors： Senerovic L,Stankovic N,Spizzo P,Basso A,Gardossi L,Vasiljevic B,Ljubijankic G,Tisminetzky S,Degrassi G

更新日期：2006-02-05 00:00:00

abstract：:The effect of different ammonium concentrations and glucosamine on baby hamster kidney (BHK)-21 cell cultures grown in continuously perfused double membrane bioreactors was investigated with respect to the final carbohydrate structures of a secretory recombinant glycoprotein. The human interleukin-2 (IL-2) mutant glyc...

journal_title：Biotechnology and bioengineering

authors： Gawlitzek M,Valley U,Wagner R

更新日期：1998-03-05 00:00:00

abstract：:A conceptual model describing the relative roles of sorption and precipitation processes for metals in solid-solution suspensions is presented. The model performance is demonstrated using experimental data on sorption and precipitation of metals in samples of activated sludge mixed liquor. Based on the experimental re...

journal_title：Biotechnology and bioengineering

authors： Kodukula PS,Patterson JW,Surampalli RY

更新日期：1994-04-15 00:00:00

abstract：:A successful procedure for attaching cellulase to 45 microm iron oxide particles with a high-molecular-weight (1000) ligand has been developed. Mass and activity balances were calculated for immobilized enzymes with different loadings. The highest specific activity of the immobilized enzyme was 5.9 mmol glucose/g boun...

journal_title：Biotechnology and bioengineering

authors： Garcia A 3rd,Oh S,Engler CR

更新日期：1989-01-15 00:00:00

abstract：:A simple approach was developed to determine the half-saturation coefficient for dissolved oxygen (K(DO)) for three bacteria by maintaining a constant oxygen concentration in continuous culture, and employing a dynamic method to obtain the specific growth rate (mu) for each species. Measurement of mu at selected disso...

journal_title：Biotechnology and bioengineering

authors： Hao OJ,Richard MG,Jenkins D,Blanch HW

更新日期：1983-02-01 00:00:00

abstract：:We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...

journal_title：Biotechnology and bioengineering

authors： Fu J,VanDusen WJ,Kolodin DG,O'Keefe DO,Herber WK,George HA

更新日期：1996-03-05 00:00:00

abstract：:This research investigates the effect of solids retention time (SRT) on the acid-phase anaerobic digestion of primary sludge. A series of experiments were conducted using two continuous-flow 3-L units with the following configuration: a completely mixed reactor (CMR) with clarifier and solids recycle and an upflow ana...

journal_title：Biotechnology and bioengineering

authors： Elefsiniotis P,Oldham WK

更新日期：1994-06-05 00:00:00

abstract：:Among the host of substratum properties that affect animal cell behavior, surface morphology has received relatively little attention. The earliest effect of surface morphology on animal cells was discovered almost a century ago when it was found that cells became oriented in response to the underlying topography. Thi...

journal_title：Biotechnology and bioengineering

authors： Singhvi R,Stephanopoulos G,Wang DI

更新日期：1994-04-05 00:00:00

abstract：:Hexanal is a key organoleptic element of green-note that is found in both fragrances and flavors. We report a novel process for the production of hexanal using immobilized enzyme templates extracted from different plant sources in combination with hollow-fiber ultrafiltration for in situ separation. Enzyme templates, ...

journal_title：Biotechnology and bioengineering

authors： Schade F,Thompson JE,Legge RL

更新日期：2003-11-05 00:00:00

abstract：:Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and gro...

journal_title：Biotechnology and bioengineering

authors： Domingues L,Lima N,Teixeira JA

更新日期：2001-03-05 00:00:00

abstract：:CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...

journal_title：Biotechnology and bioengineering

authors： deZengotita VM,Schmelzer AE,Miller WM

更新日期：2002-02-15 00:00:00

abstract：:Wastewater-fed microbial fuel cells (MFCs) are a promising technology to treat low-organic carbon wastewater and recover part of the chemical energy in wastewater as electrical power. However, the interactions between electrochemically active and fermentative microorganisms cannot be easily studied in wastewater-fed M...

journal_title：Biotechnology and bioengineering

authors： Bourdakos N,Marsili E,Mahadevan R

更新日期：2014-04-01 00:00:00

abstract：:Hydroxycinnamic acids are known to inhibit microbial growth during fermentation of lignocellulosic biomass hydrolysates, and the ability to diminish hydroxycinnamic acid toxicity would allow for more effective biological conversion of biomass to fuels and other value-added products. In this work, we provide a proof-of...

journal_title：Biotechnology and bioengineering

authors： Tomek KJ,Saldarriaga CR,Velasquez FP,Liu T,Hodge DB,Whitehead TA

更新日期：2015-03-01 00:00:00

abstract：:The antibiotic, difficidin, and its hydroxylated derivative oxydifficidin, were synthesized by cultures of Bacillus subtilis grown on a complex medium. Maximum titers of about 200 and 130 mg/L, respectively, were obtained. In fermentations where the dissolved oxygen tension (DOT) was controlled, the maximum specific g...

journal_title：Biotechnology and bioengineering

authors： Suphantharika M,Ison AP,Lilly MD,Buckland BC

更新日期：1994-10-01 00:00:00

abstract：:A whole cell-based optical sensing system for copper was developed based on Saccharomyces cerevisiae cells harboring plasmid pYEX-GFPuv. The basis of this system was the ability of the transcriptional activator protein Ace1 present in S. cerevisiae to control the expression of the reporter protein, GFPuv. When copper ...

journal_title：Biotechnology and bioengineering

authors： Shetty RS,Deo SK,Liu Y,Daunert S

更新日期：2004-12-05 00:00:00

abstract：:Recombinant cell growth and protein synthesis by a recombinant Escherichia coli under various inducing conditions are compared to the predictions of a mathematical model. The mathematical model used was a combination of two literature models: (1) an empirical kinetic model for recombinant growth and product formation ...

journal_title：Biotechnology and bioengineering

authors： Betenbaugh MJ,Dhurjati P

更新日期：1990-06-20 00:00:00

abstract：:Pichia pastoris, a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanol-induced AOX1 promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes glycerol and biomass is accumulated; in th...

journal_title：Biotechnology and bioengineering

authors： Trinh LB,Phue JN,Shiloach J

更新日期：2003-05-20 00:00:00

abstract：:Affinity chromatography using immobilized S-protein was used for the screening of affinity peptide ligands from two soluble peptide libraries. Peptide library I consisted of octamers with glycine (G) at both termini of each peptide, i.e. GXXXXXXG. The six center positions were constructed using random sequences of six...

journal_title：Biotechnology and bioengineering

authors： Huang PY,Carbonell RG

更新日期：1999-06-20 00:00:00

abstract：:The Model presented in this work demonstrates the combination of cell-cycle model with a model describing the growth and conversion kinetics of hybridoma cells in a steady-state continuous culture. The cell-cycle model is based upon a population balance model as described by Cazzador et al. and assumes the existence o...

journal_title：Biotechnology and bioengineering

authors： Martens DE,Sipkema EM,de Gooijer CD,Beuvery EC,Tramper J

更新日期：1995-10-05 00:00:00

abstract：:The production of beta-galactosidase from the E. coli phoA-lacZ fusion gene was studied to compare the gene expression behavior of two cloning methods: insertion to multicopy plasmids and integration into host cell's chromosome. The chromosome-integrating strain showed more tight control of fusion gene expression leve...

journal_title：Biotechnology and bioengineering

authors： Shin PK,Seo JH

更新日期：1990-12-20 00:00:00

abstract：:Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...

journal_title：Biotechnology and bioengineering

authors： Torisawa YS,Nashimoto Y,Yasukawa T,Shiku H,Matsue T

更新日期：2007-06-15 00:00:00

abstract：:The heterogeneous nature of hollow fiber reactors for cell cultivation requires special considerations for proper design and operation. Downstream concentration of high-molecular-weight proteins has been measured in the shell side of ultrafiltration hollow fiber bioreactors. This distribution resulted from shell-side ...

journal_title：Biotechnology and bioengineering

authors： Piret JM,Cooney CL

更新日期：1990-11-01 00:00:00

abstract：:Cell lines derived from Trichoplusia ni (Tn) are widely used as hosts in the baculovirus-insect cell system (BICS). One advantage of Tn cell lines is they can produce recombinant proteins at higher levels than cell lines derived from other insects. However, Tn cell lines are persistently infected with an alphanodaviru...

journal_title：Biotechnology and bioengineering

authors： Maghodia AB,Geisler C,Jarvis DL

更新日期：2020-07-14 00:00:00

abstract：:In glutamic acid fermentation, the molasses feeding policy and time of penicillin addition significantly affected glutamic acid production, and a fuzzy supervisory control system was developed for their quasi-optimal regulation.From the trend of the experimental data, production rules and membership functions of fuzzy...

journal_title：Biotechnology and bioengineering

authors： Kitsuta Y,Kishimoto M

更新日期：1994-06-05 00:00:00