The influence of dissolved oxygen tension on the synthesis of the antibiotic difficidin by bacillus subtilis.

abstract：:Penicillinase (beta-lactamase I, EC 3.5.2.6) secreted by Bacillus cereus, strain 569/H, was covalently attached to aminoethyl cellulose via glutaraldehyde. The immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. T...

journal_title：Biotechnology and bioengineering

authors： Klemes Y,Citri N

更新日期：1979-05-01 00:00:00

abstract：:In regenerative medicine, computer models describing bioreactor processes can assist in designing optimal process conditions leading to robust and economically viable products. In this study, we started from a (3D) mechanistic model describing the growth of neotissue, comprised of cells, and extracellular matrix, in a...

journal_title：Biotechnology and bioengineering

authors： Mehrian M,Guyot Y,Papantoniou I,Olofsson S,Sonnaert M,Misener R,Geris L

更新日期：2018-03-01 00:00:00

abstract：:Copolymers of N-isopropylacrylamide and N-acryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (Ki) of these polymers for trypsin de...

journal_title：Biotechnology and bioengineering

authors： Vaidya AA,Lele BS,Kulkarni MG,Mashelkar RA

更新日期：1999-08-20 00:00:00

abstract：:The effect of hydrogen and carbon dioxide partial pressure on the growth of the extremely thermophilic archaebacterium Pyrodictium brockii at 98 degrees C was investigated. Previous work with this bacterium has been done using an 80:20 hydrogen-carbon dioxide gas phase with a total pressure of 4 atm; no attempt has be...

journal_title：Biotechnology and bioengineering

authors： Parameswaran AK,Schicho RN,Soisson JP,Kelly RM

更新日期：1988-08-05 00:00:00

abstract：:Static and dynamic characteristics of continuous cultures of recombinant methylotrophs, which are designed to improve the selectivity of plasmid-bearing cells and the plasmid stability, are investigated in detail. Operational regions in which coexistence (survival of plasmid-bearing and plasmid-free cells) operation i...

journal_title：Biotechnology and bioengineering

authors： Parulekar SJ,Chang YK,Modak JM,Lim HC

更新日期：1987-06-01 00:00:00

abstract：:Bamboo carbohydrates were hydrolyzed with commercial amylases and a mixture of fungal culture broths containing cellulolytic and hemicellulolytic enzymes. The effects of cooking temperature and the size of fiber particles were also investigated. It was found that the higher the cooking temperature, the higher the rate...

journal_title：Biotechnology and bioengineering

authors： De Menezes TJ,Dos Santos CL,Azzini A

更新日期：1983-04-01 00:00:00

abstract：:The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...

journal_title：Biotechnology and bioengineering

authors： Dehghani F,Annabi N,Titus M,Valtchev P,Tumilar A

更新日期：2009-02-01 00:00:00

abstract：:Many microorganisms are capable of sequestering and concentrating heavy metals from their aqueous environment. While much research has beep carried out on the uptake of single species of metal ions, little attention seems to have been given to the study of multimetal ion systems. A mathematical model has previously be...

journal_title：Biotechnology and bioengineering

authors： Ting YP,Prince IG,Lawson F

更新日期：1991-03-05 00:00:00

abstract：:The clustered regularly interspaced short palindromic repeats (CRISPR) system-associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering...

journal_title：Biotechnology and bioengineering

authors： Pazmiño-Ibarra V,Mengual-Martí A,Targovnik AM,Herrero S

更新日期：2019-11-01 00:00:00

abstract：:Protein-protein interactions were measured for ovalbumin and for lysozyme in aqueous salt solutions. Protein-protein interactions are correlated with a proposed potential of mean force equal to the free energy to desolvate the protein surface that is made inaccessible to the solvent due to the protein-protein interact...

journal_title：Biotechnology and bioengineering

authors： Curtis RA,Ulrich J,Montaser A,Prausnitz JM,Blanch HW

更新日期：2002-08-20 00:00:00

abstract：:The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral dow...

journal_title：Biotechnology and bioengineering

authors： Nestola P,Peixoto C,Silva RR,Alves PM,Mota JP,Carrondo MJ

更新日期：2015-05-01 00:00:00

abstract：:A titration bioassay, designed to accurately determine the activity of acetoclastic methanogens, is described that also allows evaluation of inhibition due to potential toxicants on the active biomass. The instrument is made of a pH-stat connected to an anaerobic batch reactor. Acetate is blended and mixed with anaero...

journal_title：Biotechnology and bioengineering

authors： Rozzi A,Castellazzi L,Speece RE

更新日期：2002-01-05 00:00:00

abstract：:The laminate consisted of several polymer layers, aluminium, and one cellulose-based layer containing the active enzymatic system (e.g., glucose oxidase, catalase, glucose, and CaCO3). During the industrial lamination process, the enzyme layer was exposed to three temperature spikes up to 325 degrees C without signifi...

journal_title：Biotechnology and bioengineering

authors： Andersson M,Andersson T,Adlercreutz P,Nielsen T,Hörnsten EG

更新日期：2002-07-05 00:00:00

abstract：:The effector functions elicited by IgG antibodies strongly depend on the carbohydrate moiety linked to the Fc region of the protein. Therefore several approaches have been developed to rationally manipulate these glycans and improve the biological functions of the antibody. Overexpression of recombinant beta1,4-N-acet...

journal_title：Biotechnology and bioengineering

authors： Ferrara C,Brünker P,Suter T,Moser S,Püntener U,Umaña P

更新日期：2006-04-05 00:00:00

abstract：:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...

journal_title：Biotechnology and bioengineering

authors： Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

更新日期：2008-08-01 00:00:00

abstract：:Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

journal_title：Biotechnology and bioengineering

authors： Vemuri GN,Eiteman MA,Altman E

更新日期：2006-06-20 00:00:00

abstract：:Embryonic stem cells (ESCs) can differentiate into all somatic cell types, but the development of effective strategies to direct ESC fate is dependent upon defining environmental parameters capable of influencing cell phenotype. ESCs are commonly differentiated via cell aggregates referred to as embryoid bodies (EBs),...

journal_title：Biotechnology and bioengineering

authors： Sargent CY,Berguig GY,Kinney MA,Hiatt LA,Carpenedo RL,Berson RE,McDevitt TC

更新日期：2010-02-15 00:00:00

abstract：:Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phas...

journal_title：Biotechnology and bioengineering

authors： Lander RJ,Winters MA,Meacle FJ,Buckland BC,Lee AL

更新日期：2002-09-30 00:00:00

abstract：:A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysis focuses on a tim...

journal_title：Biotechnology and bioengineering

authors： Rizzi M,Baltes M,Theobald U,Reuss M

更新日期：1997-08-20 00:00:00

abstract：:The thermophilic fungus Sporotrichum cellulophilum was immobilized with nonwoven materials for cellulase production. The cellulose powder concentration in the medium was an important factor controlling cellulase production. When the cellulose powder concentration in the nonwoven materials was more than 4%, cellulase p...

journal_title：Biotechnology and bioengineering

authors： Tamada M,Kasai N,Kumakura M,Kaetsu I

更新日期：1986-08-01 00:00:00

abstract：:The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that converts waste oils or fats into a series of value-added products. Our research employs the oleaginous yeast Yarrowia lipolytica as a case study for citric acid (CA) ...

journal_title：Biotechnology and bioengineering

authors： Liu N,Soong YV,Mirzaee I,Olsen A,Yu P,Wong HW,Xie D

更新日期：2021-01-20 00:00:00

abstract：:The redox control bioreactor (RCB) is a new hollow fiber membrane bioreactor (HFMBR) design in which oxygen and hydrogen gases are provided simultaneously through separate arrays of juxtaposed hollow fiber (HF) membranes. This study applied the RCB for completely autotrophic conversion of ammonia to N(2) through nitri...

journal_title：Biotechnology and bioengineering

authors： Smith DP,Rector T,Reid-Black K,Hummerick M,Strayer R,Birmele M,Roberts MS,Garland JL

更新日期：2008-03-01 00:00:00

abstract：:Bacterial magnetic particles (BMPs) were used for the identification of cyanobacterial DNA. Genus-specific oligonucleotide probes for the detection of Anabaena spp., Microcystis spp., Nostoc spp., Oscillatoria spp., and Synechococcus spp. were designed from the variable region of the cyanobacterial 16S rDNA of 148 str...

journal_title：Biotechnology and bioengineering

authors： Matsunaga T,Nakayama H,Okochi M,Takeyama H

更新日期：2001-06-05 00:00:00

abstract：:Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB pro...

journal_title：Biotechnology and bioengineering

authors： Tanadchangsaeng N,Yu J

更新日期：2012-11-01 00:00:00

abstract：:Chlorella sp. strain VJ79 was isolated from a total heterotrophic count of a wastewater collector. It grows autotrophically, heterotrophically, and mixotrophically on a variety of organic substrates. Glucose and serine promote a mixotrophic growth from which the yield is higher than the sum of autotrophic and heterotr...

journal_title：Biotechnology and bioengineering

authors： Lalucat J,Imperial J,Parés R

更新日期：1984-07-01 00:00:00

abstract：:A bubble column (0.05 m(3)) and an air-lift fermentor (1.2 m(3)) were used for the production of the exocellular microbial polysaccharide xanthan with Xanthomonas campestris in a synthetic medium. Upon oxygen depletion in the liquid, the xanthan production rate dropped sharply and then became a linear function of the ...

journal_title：Biotechnology and bioengineering

authors： Suh IS,Schumpe A,Deckwer WD

更新日期：1992-01-05 00:00:00

abstract：:Although several compelling benefits for bioprocess intensification have been reported, the need for a streamlined integration of perfusion cultures with capture chromatography still remains unmet. Here, a robust solution is established by conducting tangential flow filtration-based perfusion with a wide-surface pore ...

journal_title：Biotechnology and bioengineering

authors： Pinto NDS,Brower M

更新日期：2020-07-15 00:00:00

abstract：:This paper proposes a new metabolic model for acetate uptake by a mixed culture of phosphate- and glycogen-accumulating organisms (PAOs and GAOs) under anaerobic conditions. The model uses variable overall stoichiometry based on the assumption that PAOs may have the ability of using the glyoxylate pathway to produce t...

journal_title：Biotechnology and bioengineering

authors： Yagci N,Artan N,Cokgör EU,Randall CW,Orhon D

更新日期：2003-11-05 00:00:00

abstract：:The effects of dilution rates on the performance of a two-stage fermentation system for a recombinant Escherichia coli culture were studied. Dilution rate determines the apparent or averaged specific growth rate of a heterogeneous population of cells in the recombinant culture. The specific growth rate affects the gen...

journal_title：Biotechnology and bioengineering

authors： Hortacsu A,Ryu DD

更新日期：1991-10-20 00:00:00

abstract：:Intracellular delivery of functional proteins is of great interest for basic biological research as well as for clinical applications. Transfection is the most commonly used method, however, it is not applicable to large-scale manipulation and inefficient in important cell types implicated in biomedical applications, ...

journal_title：Biotechnology and bioengineering

authors： Li Z,Cai Z,Fu W,Liu Y,Tian C,Wang H,Fu T,Wu Z,Wu D,Jin Y,Cheng Z,Terada N,Liu L,Wu W,Jin S,Bai F

更新日期：2020-03-01 00:00:00