Immobilization of yeast cells containing beta-galactosidase.

Abstract:

:Cultural conditions optimum for beta-galactosidase production by Saccharomyces anamensis are pH 4.5, temperature 26 +/- 2 degrees C, and 30 h of incubation period. Addition of lactose at 24 h fermentation greatly increase the level of enzyme. Optimum pHl, temperature, pH stability, and thermostability of yeast beta-galactosidase are negligibly affected by immobilization. The K(m) values of enzyme in the native and immobilized cells are 102mM and 148mM, respectively. Glucose noncompetitively inhibits the enzyme activity. Addition of substances such as dithioerythritol, glutathione, and bovine serum albumin to the native cell during assay procedure and immobilized cell prior to immobilization have stimulatory effects on enzyme activity. Metal ions like Ca(2+), Mg(2+) enhance the beta-galactosidase activity for both intact and bound cells. Immobilized cells retain 68.6% of the beta-galactosidase activity of intact cells and there is no significant loss of activity on storage at 4 degrees C for 28 days.

journal_name

Biotechnol Bioeng

authors

Banerjee M,Chakrabarty A,Majumdar SK

doi

10.1002/bit.260240810

subject

Has Abstract

pub_date

1982-08-01 00:00:00

pages

1839-50

issue

8

eissn

0006-3592

issn

1097-0290

journal_volume

24

pub_type

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