Immobilization of yeast cells containing beta-galactosidase.


:Cultural conditions optimum for beta-galactosidase production by Saccharomyces anamensis are pH 4.5, temperature 26 +/- 2 degrees C, and 30 h of incubation period. Addition of lactose at 24 h fermentation greatly increase the level of enzyme. Optimum pHl, temperature, pH stability, and thermostability of yeast beta-galactosidase are negligibly affected by immobilization. The K(m) values of enzyme in the native and immobilized cells are 102mM and 148mM, respectively. Glucose noncompetitively inhibits the enzyme activity. Addition of substances such as dithioerythritol, glutathione, and bovine serum albumin to the native cell during assay procedure and immobilized cell prior to immobilization have stimulatory effects on enzyme activity. Metal ions like Ca(2+), Mg(2+) enhance the beta-galactosidase activity for both intact and bound cells. Immobilized cells retain 68.6% of the beta-galactosidase activity of intact cells and there is no significant loss of activity on storage at 4 degrees C for 28 days.


Biotechnol Bioeng


Banerjee M,Chakrabarty A,Majumdar SK




Has Abstract


1982-08-01 00:00:00












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    journal_title:Biotechnology and bioengineering

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    authors: Graf von der Schulenburg DA,Holland DJ,Paterson-Beedle M,Macaskie LE,Gladden LF,Johns ML

    更新日期:2008-03-01 00:00:00

  • Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest.

    abstract::We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...

    journal_title:Biotechnology and bioengineering

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    authors: Droz X,Harraghy N,Lançon E,Le Fourn V,Calabrese D,Colombet T,Liechti P,Rida A,Girod PA,Mermod N

    更新日期:2017-08-01 00:00:00

  • Development of waxy cassava with different Biological and physico-chemical characteristics of starches for industrial applications.

    abstract::The quality of cassava starch, an important trait in cassava breeding programs, determines its applications in various industries. For example, development of waxy (having a low level of amylose) cassava is in demand. Amylose is synthesized by granule-bound starch synthase I (GBSSI) in plants, and therefore, down-regu...

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    authors: Zhao SS,Dufour D,Sánchez T,Ceballos H,Zhang P

    更新日期:2011-08-01 00:00:00

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    journal_title:Biotechnology and bioengineering

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    authors: Grosz R,Stephanopoulos G

    更新日期:1990-12-05 00:00:00

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    abstract::The yeast Pachysolen tannophilus was entrapped in calcium alginate beads to ferment D-xylose on a continous basis in the presence of high cell densities. Experimental operating variables included the feed D-xylose concentration, the dilution rate, and the fermentor biomass concentration. Under favorable operating cond...

    journal_title:Biotechnology and bioengineering

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    authors: Slininger PJ,Bothast RJ,Black LT,McGhee JE

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    authors: Scott CD,Hancher CW

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    abstract::Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

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    authors: Johansson L,Lindskog A,Silfversparre G,Cimander C,Nielsen KF,Lidén G

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    pub_type: 杂志文章


    authors: Vasiliev VB,Vavilin VA

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