Abstract:
:Studies have been conducted in a gas circulation type fermentor in order to characterize the ethanol fermentation of uncooked cassava starch with Rhizopus koji. Results showed that ethanol concentration reached 13-14% (v/v) in 4-day broth, and the maximum productivity of ethanol was 2.3 g ethanol/L broth h. This productivity was about 50% compared to the productivity of a glucose-yeast system. Ethanol yield reached 83.5-72.3% of the theoretical yield for the cassava starch used. The fermentor used in the present work has been proven by experiment to be suitable for ethanol fermentation of the broth with solid substrate.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Fujio Y,Ogata M,Ueda Sdoi
10.1002/bit.260270823subject
Has Abstractpub_date
1985-08-01 00:00:00pages
1270-3issue
8eissn
0006-3592issn
1097-0290journal_volume
27pub_type
杂志文章abstract::The Ff filamentous bacteriophages show potential as a new class of therapeutics, displaying utility in materials science as well as pharmaceutical applications. These phages are produced by the infection of E. coli, a Gram-negative bacterium which unavoidably sheds endotoxins into the extracellular space during growth...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25571
更新日期:2015-08-01 00:00:00
abstract::Porcine pancreatic lipase and Fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390415
更新日期:1992-02-20 00:00:00
abstract::An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22250
更新日期:2009-06-01 00:00:00
abstract::A modified mixing plane approach for steady state simulation of flow field in fully baffled biological reactor is presented and discussed. Without requiring any experimental input, this approach of dividing the vessel into suitable number of connected and disconnected zones; solving steady state equation separately in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21030
更新日期:2006-11-05 00:00:00
abstract::A model was developed to evaluate the effects of cells and surfactants on oxygen transfer in surface-aerated bioreactors. The model assumed the presence of serial layers of adsorbed surfactants and microorganisms directly adjacent to the gas-liquid interface due to their surface activities, followed by a stagnant liqu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260401108
更新日期:1992-12-20 00:00:00
abstract::A kinetic model describing the cephalosporin biosynthesis in Streptomyces clavuligerus was developed. Using previously reported kinetic data of biosynthetic enzymes, we examined the kinetics of cephalosporin production. The predicted time profile of the specific production rate during a batch culture parallels that of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380815
更新日期:1991-10-20 00:00:00
abstract::High hydrostatic pressure (HHP) stabilized glucose oxidase (GOx) against thermal inactivation. The apparent first-order kinetics of inactivation of GOx were investigated at 0.1-300 MPa and 58.8-80.0°C. At 240 MPa and 74.5°C, GOx inactivated at a rate 50 times slower than at atmospheric pressure at the same temperature...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26185
更新日期:2017-03-01 00:00:00
abstract::A titration bioassay, designed to accurately determine the activity of acetoclastic methanogens, is described that also allows evaluation of inhibition due to potential toxicants on the active biomass. The instrument is made of a pH-stat connected to an anaerobic batch reactor. Acetate is blended and mixed with anaero...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10088
更新日期:2002-01-05 00:00:00
abstract::As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27311
更新日期:2020-06-01 00:00:00
abstract::The application of rational design in reallocating metabolic flux to accumulate desired chemicals is always restricted by the native regulatory network. In this study, recombinant Pichia pastoris was constructed for malic acid production from sole methanol through rational redistribution of metabolic flux. Different m...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27575
更新日期:2021-01-01 00:00:00
abstract::Microbial kinetics and energetics are discussed in connection with the formulation of unstructured growth models. The development of microbial energetics and the use of macroscopic methods in the study of microbial growth are briefly evaluated. The general approach to the modelling of microbial growth has been critica...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251202
更新日期:1983-12-01 00:00:00
abstract::Mechanical forces regulate the structure and function of many tissues in vivo; recent results indicate that the mechanical environment can decisively influence the development of engineered tissues cultured in vitro. To investigate the effects of the hydrodynamic environment on tissue-engineered cartilage, primary bov...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20000220)72:4<402::aid-bit1002>3
更新日期:2001-02-20 00:00:00
abstract::Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22765
更新日期:2010-08-15 00:00:00
abstract::The hollow fiber enzyme reactor with pulsation developed by Kim and Chang (1983) was operated in a differential mode by recycling a substrate solution, in order to assess the efficiency of ultrafiltration swing. The rates of lactose conversion by beta-galactosidase contained in the shell side of the reactor were measu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270813
更新日期:1985-08-01 00:00:00
abstract::Flow cytometry is a fast and sensitive method that allows monitoring of different cellular parameters on large samples of a population. Protein distributions give relevant information on growth dynamics, since they are related to the age distribution and depend on the law of growth of the population and the law of pro...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250510
更新日期:1983-05-01 00:00:00
abstract::Metabolically active resting (i.e., nongrowing) bacterial cells have a high potential in cofactor-dependent redox biotransformations. Where growing cells require carbon and energy for biomass production, resting cells can potentially exploit their metabolism more efficiently for redox biocatalysis allowing higher spec...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24404
更新日期:2012-05-01 00:00:00
abstract::It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25912
更新日期:2016-07-01 00:00:00
abstract::The analysis of a continuous, aerobic, fixed-film bioreactor is performed by simulating the behavior of penicillin production in a three-phase fluidized bed. Rigorous mathematical models are developed for a fluidized-bed fermentor in which bioparticles are fluidized by the liquid medium and air. The steady-state perfo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260509
更新日期:1984-05-01 00:00:00
abstract::A model is developed to predict batch and continuous culture behavior of fermentations on two different carbon and energy sources. The basic assumption of the model is that the permease for the favored substrate is constitutive, whereas the premease for the second substrate is subject to induction and catabolite repre...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260170905
更新日期:1975-09-01 00:00:00
abstract::A model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. This model accounts for both charge-independent and electrostatic effects. The determination of nonelectrostatic effects was based on the model of Eiteman and Gainer for uncharged solutes while the electrostat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19971105)56:3<248::AID-BIT
更新日期:1997-11-05 00:00:00
abstract::Air emissions from the pulp and paper industry frequently contain reduced sulfur compounds (RSC), such as dimethyl sulfide (DMS) mixed with volatile organic compounds (VOC) (e.g., methanol, MeOH) and it is desirable to treat either one or both of these groups of compounds. The objective of this study was to assess the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21033
更新日期:2006-11-05 00:00:00
abstract::We investigated the enzymic activity of bilirubin oxidase when it is suspended as a lyophilized powder in a low-water system. The enzyme required buffer salts and a source of water to show activity. This study investigated the complete range of water thermodynamic activity (a(w)) by combining the use of salt hydrates ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10103
更新日期:2002-03-20 00:00:00
abstract::Axial and radial oxygen depletion are believed to be critical scale-limiting factors in the design of cell culture hollow fiber bioreactors. A mathematical analysis of oxygen depletion has been performed in order to develop effectiveness factor plots to aid in the scaling of hollow fiber bioreactors with cells immobil...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370112
更新日期:1991-01-05 00:00:00
abstract::Saccharomyces cerevisiae (yeast) cells were employed as a source of alcohol dehydrogenase in the NAD(+)-to-NADH reaction. The cells were immobilized in calcium alginate monofilament fibers and used in a biological reactor. The alginate could not be heat sterilized since temperatures above 80 degrees C caused the polym...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360706
更新日期:1990-10-05 00:00:00
abstract::Macroencapsulation technology has been an attractive topic in the field of treatment for Type 1 diabetes due to mechanical stability, versatility, and retrievability of the macro-capsule design. Macro-capsules can be categorized into extravascular and intravascular devices, in which solute transport relies either on d...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.25895
更新日期:2016-07-01 00:00:00
abstract::A two-member methane-utilizing mixed culture of bacteria, formed by combining two pure cultures isolated from a naturally occurring methane-utilizing mixed culture, was studied in continuous culture. From the nutritional requirements and substrate ranges of the pure cultures, a mechanism for the interspecific interact...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260221010
更新日期:1980-10-01 00:00:00
abstract::Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10166
更新日期:2002-03-30 00:00:00
abstract::Remarkable increases in enzyme catalytic stability resulting from addition of charged water-soluble polymers have recently been reported, suggesting that use of these polymers may be an attractive general strategy for enzyme stabilization. To test the proposed hypothesis that coulombic forces between water-soluble pol...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1187
更新日期:2001-11-01 00:00:00
abstract::Recombinant cell growth and protein synthesis by a recombinant Escherichia coli under various inducing conditions are compared to the predictions of a mathematical model. The mathematical model used was a combination of two literature models: (1) an empirical kinetic model for recombinant growth and product formation ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360204
更新日期:1990-06-20 00:00:00
abstract::Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20277
更新日期:2004-11-20 00:00:00