Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography.

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00

abstract：:Modern large-scale agricultural practices that incorporate high density farming with subtherapeutic antibiotic dosing are considered a major contributor to the rise of antibiotic-resistant bacterial infections of humans with species of Salmonella being a leading agriculture-based bacterial infection. Microcin J25, a p...

journal_title：Biotechnology and bioengineering

authors： Ritter SC,Yang ML,Kaznessis YN,Hackel BJ

更新日期：2018-10-01 00:00:00

abstract：:Immobilized enzyme hollow fibers may be useful in the purification or treatment of whole blood under clinical conditions. In this study, catalytically pure heparinase was immobilized to cellulose to analyze the feasibility for the removal of heparin's anticoagulant activity from whole blood. The kinetics of catalytica...

journal_title：Biotechnology and bioengineering

authors： Comfort AR,Albert E,Langer R

更新日期：1989-12-20 00:00:00

abstract：:The feasibility of anaerobic methanethiol (MT) degradation at elevated sodium concentrations was investigated in a mesophilic (30 degrees C) lab-scale upflow anaerobic sludge bed (UASB) reactor, inoculated with estuarine sediment originating from the Wadden Sea (The Netherlands). MT was almost completely degraded (>95...

journal_title：Biotechnology and bioengineering

authors： van Leerdam RC,de Bok FA,Lens PN,Stams AJ,Janssen AJ

更新日期：2007-09-01 00:00:00

abstract：:The diffusivities of glucose and ethanol in cell-free and cell-occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol...

journal_title：Biotechnology and bioengineering

authors： Hannoun BJ,Stephanopoulos G

更新日期：1986-06-01 00:00:00

abstract：:Formaldehyde is a prevalent environmental toxin and a key intermediate in single carbon metabolism. The ability to monitor formaldehyde concentration is, therefore, of interest for both environmental monitoring and for metabolic engineering of native and synthetic methylotrophs, but current methods suffer from low sen...

journal_title：Biotechnology and bioengineering

authors： Woolston BM,Roth T,Kohale I,Liu DR,Stephanopoulos G

更新日期：2018-01-01 00:00:00

abstract：:The treatment of large segmental bone defects remains a challenge as infection, delayed union, and nonunion are common postoperative complications. A three-dimensional printed bioresorbable and physiologically load-sustaining graft substitute was developed to mimic native bone tissue for segmental bone repair. Fabrica...

journal_title：Biotechnology and bioengineering

authors： Chung R,Kalyon DM,Yu X,Valdevit A

更新日期：2018-09-01 00:00:00

abstract：:We report on a simple method to rapidly generate very large libraries of genes encoding mutant proteins without the use of DNA amplification, and the application of this methodology in the construction of synthetic immunoglobulin variable heavy (V(H)) and light (V(kappa)) libraries. Four high quality, chemically synth...

journal_title：Biotechnology and bioengineering

authors： Ge X,Mazor Y,Hunicke-Smith SP,Ellington AD,Georgiou G

更新日期：2010-06-15 00:00:00

abstract：:Porcine pancreatic lipase and Fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrat...

journal_title：Biotechnology and bioengineering

authors： Parvaresh F,Robert H,Thomas D,Legoy MD

更新日期：1992-02-20 00:00:00

abstract：:We present a mathematical model which includes TNF-alpha initiated survival and apoptotic cascades, as well as nuclear transcription of IkappaB. These pathways play a crucial role in deciding cell fate in response to inflammation and infection. Our model incorporates known specific protein-protein interactions as iden...

journal_title：Biotechnology and bioengineering

authors： Rangamani P,Sirovich L

更新日期：2007-08-01 00:00:00

abstract：:The development of a lentiviral system to deliver genes to specific cell types could improve the safety and the efficacy of gene delivery. Previously, we have developed an efficient method to target lentivectors to specific cells via an antibody-antigen interaction in vitro and in vivo. We report herein a targeted len...

journal_title：Biotechnology and bioengineering

authors： Froelich S,Ziegler L,Stroup K,Wang P

更新日期：2009-09-01 00:00:00

abstract：:The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5-norbornene-2.3-dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with ...

journal_title：Biotechnology and bioengineering

authors： Büttner W,Becker M,Rupprich C,Boeden HF,Henklein P,Loth F,Dautzenberg H

更新日期：1989-01-05 00:00:00

abstract：:Three micronization techniques, based on the use of supercritical carbon dioxide, were investigated to produce microspheres of a natural biocompatible polysaccharide. Particles smaller than 20 mum were obtained by means of the rapid expansion of a supercritical solution method (RESS), both with and without cosolvents....

journal_title：Biotechnology and bioengineering

authors： Benedetti L,Bertucco A,Pallado P

更新日期：1997-01-20 00:00:00

abstract：:In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charg...

journal_title：Biotechnology and bioengineering

authors： Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

更新日期：2002-07-20 00:00:00

abstract：:The effective diffusivity of oxygen, D(e), in Ca-alginate and PVA-SbQ gels was measured using a two-chamber vessel with a membrane between the two chambers. The effect of cell density, C(c), on D(e) in Ca-alginate gels was studied. The effective diffusivity of oxygen decreased with increasing cell density, to C(c) = 1...

journal_title：Biotechnology and bioengineering

authors： Sun Y,Furusaki S,Yamauchi A,Ichimura K

更新日期：1989-06-05 00:00:00

abstract：:The treatment of meta- and para-nitrobenzoic acid in an industrial wastestream by Comamonas sp. JS46 and Comamonas sp. JS47 is investigated. The most important feature of the wastestream is the constantly changing concentration ratio of the two isomers. The most extreme occurrence is considered here: the complete chan...

journal_title：Biotechnology and bioengineering

authors： Goodall JL,Thomas SM,Spain JC,Peretti SW

更新日期：1998-07-05 00:00:00

abstract：:Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the sin...

journal_title：Biotechnology and bioengineering

authors： Seo JH,Bailey JE

更新日期：1985-02-01 00:00:00

abstract：:In this report, alpha-Amylase originating from Bacillus subtilis (liquefying type) was immobilized on partially imidoesterized polyacrylonitrile (PAN) by covalent bonding. For the preparation of immobilized alpha-amylase, which has a high activity and high stability to repeated use, the optimum conditions for the prep...

journal_title：Biotechnology and bioengineering

authors： Handa T,Hirose A,Akino T,Watanabe K,Tsuchiya H

更新日期：1983-12-01 00:00:00

abstract：:We have identified an error in the published integral form of the modified Michaelis-Menten equation that accounts for endogenous substrate production. The correct solution is presented and the error in both the substrate concentration, S, and the kinetic parameters Vm , Km , and R resulting from the incorrect solutio...

journal_title：Biotechnology and bioengineering

authors： Goudar CT

更新日期：2011-10-01 00:00:00

abstract：:The emergence of medicinal indications for stem cell therapies has seen a need to develop the manufacturing capacity for adherent cells such as mesenchymal stem cells (MSCs). One such development is in the use of microcarriers, which facilitate enhanced cell densities for adherent stem cell cultures when compared with...

journal_title：Biotechnology and bioengineering

authors： Odeleye AOO,Castillo-Avila S,Boon M,Martin H,Coopman K

更新日期：2017-09-01 00:00:00

abstract：:The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion...

journal_title：Biotechnology and bioengineering

authors： Love KR,Panagiotou V,Jiang B,Stadheim TA,Love JC

更新日期：2010-06-01 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:Numerous studies on poly γ-d-glutamicacid (γ-PGA) production have investigated terrestrial renewable sources for reducing production costs, but there are no studies using waste marine resources so far. We aimed to develop a cost-effective production method of γ-d-PGA by Bacillus sp. SJ-10 using green macroalgae (Ulva ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Lee JM,Jang WJ,Park HD,Kim YO,Kim CH,Kong IS

更新日期：2019-07-01 00:00:00

abstract：:Lignin, one of the major components of lignocellulosic biomass, plays an inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the role of lignin in pretreated hardwood, where extents of cellulose hydrolysis decrease, rather than increase with increasing severity of liquid hot water pretreatment...

journal_title：Biotechnology and bioengineering

authors： Ko JK,Kim Y,Ximenes E,Ladisch MR

更新日期：2015-02-01 00:00:00

abstract：:Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Partic...

journal_title：Biotechnology and bioengineering

authors： Kaup BA,Ehrich K,Pescheck M,Schrader J

更新日期：2008-02-15 00:00:00

abstract：:We have developed a new cell surface display system using a major outer membrane protein of Pseudomonas aeruginosa OprF as an anchoring motif. Pseudomonas fluorescens SIK W1 lipase gene was fused to the truncated oprF gene by C-terminal deletion fusion strategy. The truncated OprF-lipase fusion protein was successfull...

journal_title：Biotechnology and bioengineering

authors： Lee SH,Choi JI,Han MJ,Choi JH,Lee SY

更新日期：2005-04-20 00:00:00

abstract：:Although benzene can be biodegraded when dissolved oxygen is sufficient, delivering oxygen is energy intensive and can lead to air stripping the benzene. Anaerobes can biodegrade benzene by using electron acceptors other than O2 , and this may reduce costs and exposure risks; the drawback is a remarkably slower growth...

journal_title：Biotechnology and bioengineering

authors： Liu Z,Zhou C,Ontiveros-Valencia A,Luo YH,Long M,Xu H,Rittmann BE

更新日期：2018-08-01 00:00:00

abstract：:Enterokinase and recombinant enterokinase light chain (rEK(L)) have been used widely to cleave fusion proteins with the target sequence of (Asp)(4)-Lys. In this work, we show that their utility as a site-specific cleavage agent is compromised by sporadic cleavage at other sites, albeit at low levels. Further degradati...

journal_title：Biotechnology and bioengineering

authors： Choi SI,Song HW,Moon JW,Seong BL

更新日期：2001-12-20 00:00:00

abstract：:Experimental and theoretical studies are conducted to understand the dynamic behavior of a continuous-flow fixed-bed reactor in which an esterification is catalyzed by an immobilized enzyme in an organic solvent medium. The experimental system consists of a commercial immobilized lipase preparation known as Lipozyme a...

journal_title：Biotechnology and bioengineering

authors： Mensah P,Gainer JL,Carta G

更新日期：1998-11-20 00:00:00

abstract：:The hypothesis that frequency and amplitude response can be used in a complicated metabolic pathway kinetics model for optimal parameter estimation, as speculated by its successful prior usage for a mechanical oscillator and a heterogeneous chemical system, is tested here. Given the complexity of the glycolysis model ...

journal_title：Biotechnology and bioengineering

authors： Zimmerman WB

更新日期：2005-10-05 00:00:00