Abstract:
:The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5-norbornene-2.3-dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with the amino groups takes place with a 10-fold higher rate than the hydrolysis of the carbonate groups, and high coupling yields can be reached. It is shown that the activated supports are well suitable for the preparation of carriers for affinity chromatography or the immobilization of enzymes.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Büttner W,Becker M,Rupprich C,Boeden HF,Henklein P,Loth F,Dautzenberg Hdoi
10.1002/bit.260330105subject
Has Abstractpub_date
1989-01-05 00:00:00pages
26-31issue
1eissn
0006-3592issn
1097-0290journal_volume
33pub_type
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