Abstract:
:Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the single-cell level. The distribution of cellular plasmid content and specific rates of plasmid gene expression have been obtained for several single-cell models of plasmid replication, partition, and gene expression. Plasmid replication kinetics during cell growth significantly influence the plasmid content distribution. In the case of transient growth of plasmid-containing and plasmid-free cells in partially selective medium, the degree of selection required for stable maintenance of plasmid-containing cells has been determined. Guidelines are presented for applicability of simpler, nonsegregated models and for evaluation of the parameters in these models based on single-cell mechanisms and associated parameters.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Seo JH,Bailey JEdoi
10.1002/bit.260270209subject
Has Abstractpub_date
1985-02-01 00:00:00pages
156-66issue
2eissn
0006-3592issn
1097-0290journal_volume
27pub_type
杂志文章abstract::The purpose of this article is to provide a few concrete examples of the potential to acceptably reduce the scope of validation and qualification testing based on scientific justification for the specific area of microbial fermentation. The key areas explored include: autoclave operational qualification (OQ) testing, ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1094
更新日期:2001-07-05 00:00:00
abstract::The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22059
更新日期:2009-02-01 00:00:00
abstract::Escherichia coli and Desulfovibrio desulfuricans reduce Tc(VII) (TcO(4)(-)) with formate or hydrogen as electron donors. The reaction is catalyzed by the hydrogenase component of the formate hydrogenlyase complex (FHL) of E. coli and is associated with a periplasmic hydrogenase activity in D. desulfuricans. Tc(VII) re...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::A fuzzy-logic light attenuation model was developed and validated for a stirred-tank photobioreactor. Based on this model, local light intensities were used to calculate local specific growth rates of the cyanobacteria Synechococcus PCC 7942. The light regime for maximization of biomass space-time yield in a batch pro...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21086
更新日期:2006-12-20 00:00:00
abstract::The glycosylation patterns of recombinant therapeutic glycoproteins can be engineered by overexpression of glycosyltransferases in the host cells used for glycoprotein production. Most prior glycosylation engineering experiments have involved constitutive expression of cloned glycosyltransferases. Here we use tetracyc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1999-12-05 00:00:00
abstract::We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26270
更新日期:2017-08-01 00:00:00
abstract::In Part II, the process utility of a thermotolerant methylotrophic bacterium is evaluated with respect to its dynamic response when various substrate or nutrient pulses are imposed on it while growing under steady-state conditions in a chemostat. The pulses investigated were methanol pulses on methanol-, methanol/form...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360811
更新日期:1990-10-20 00:00:00
abstract::We Propose a kinetic expression which accounts for the temperature dependence of ethanol yield losses in batch alcoholic fermentation. Moreover, the characteristic parameters of the microbial growth equation have been calculated for Saccharomyces cerevisiae under typical wine industry conditions. A substrate consumpti...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380708
更新日期:1991-10-05 00:00:00
abstract::A microarray targeting four sequenced strains in the Dehalococcoides (Dhc) genus was used to analyze gene expression in a robust long-term trichloroethene (TCE)-degrading microbial community (designated ANAS) during feeding cycles that involve conditions of periodic substrate supply. The Dhc transcriptome was examined...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24819
更新日期:2013-05-01 00:00:00
abstract::The effect of organic solvents on the equilibrium position of lipase-catalyzed esterification of glycerol and decanoic acid has been investigated. The reaction is carried out in an aqueous-organic two-phase system. In polar solvents, high mole fractions of monoacylglycerol and low mole fractions of triacylglycerol and...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410113
更新日期:1993-01-05 00:00:00
abstract::Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20882
更新日期:2006-07-05 00:00:00
abstract::The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1167
更新日期:2001-10-05 00:00:00
abstract::The application of enantioseparation methods alone can only yield up to 50% of the desired chiral product. Thus enantioseparation becomes more attractive when accompanied by the racemization of the counter-enantiomer. Here we present first results of dynamic kinetic resolution of L-asparagine (L-Asn) via preferential ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22498
更新日期:2009-12-15 00:00:00
abstract::The diffusivities of glucose and ethanol in cell-free and cell-occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280609
更新日期:1986-06-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260310714
更新日期:1988-05-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20166
更新日期:2004-08-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10756
更新日期:2003-10-05 00:00:00
abstract::Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20001020)70:2<187::aid-bit8>3.0.
更新日期:2000-10-20 00:00:00
abstract::Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400609
更新日期:1992-09-01 00:00:00
abstract::Chlorophyllide a was coupled with alpha-(3-aminopropyl)-omega-methoxypoly(oxyethylene) (PEG-NH2) to form a PEG-chlorophyllide conjugate through an acid-amide bond. The conjugate catalyzed the reduction of methylviologen in the presence of 2-mercaptoethanol. It also catalyzed the photoreduction of NADP+ or NAD+ in the ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1029
更新日期:2001-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20509
更新日期:2005-07-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27226
更新日期:2020-03-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26780
更新日期:2018-09-01 00:00:00
abstract::Anaerobic methanethiol (MT) degradation by mesophilic (30 degrees C) alkaliphilic (pH 10) communities was studied in a lab-scale Upflow Anaerobic Sludge Bed (UASB) reactor inoculated with a mixture of sediments from the Wadden Sea (The Netherlands), Soap Lake (Central Washington), and Russian soda lakes. MT degradatio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21933
更新日期:2008-11-01 00:00:00
abstract::In this study, an analysis of the transition between the inactive ("closed") and active ("open") conformations of Candida rugosa lipase in solution is performed using irreversible enzyme inhibitors, cyclic saligenin phosphates. It is shown that >90% inhibition of the enzyme activity toward water-soluble substrates (es...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20010105)72:1<108::aid-bit14>3.0
更新日期:2001-01-05 00:00:00
abstract::Experimental observations indicate that the rates of cometabolic transformation are linked to the consumption of growth substrate during growth and to the consumption of cell mass and/or energy substrate in the absence of growth substrate. Three previously proposed models (models 1 through 3) describing the kinetics o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260411107
更新日期:1993-05-01 00:00:00
abstract::A desirable feature of many therapeutic glycoprotein production processes is to maximize the final sialic acid content. In this study, the effect of applying a novel chemical analog of the sialic acid precursor N-acetylmannosamine (ManNAc) on the sialic acid content of cellular proteins and a model recombinant glycopr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26291
更新日期:2017-08-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22136
更新日期:2009-02-15 00:00:00
abstract::We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960305)49:5<578::AID-BIT
更新日期:1996-03-05 00:00:00
abstract::Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(e...
journal_title:Biotechnology and bioengineering
pub_type: 信件
doi:10.1002/bit.22997
更新日期:2011-04-01 00:00:00