Protein A-based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme-linked immunosorbent assay.


:Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity of ELISA in poly-(methyl methacrylate) (PMMA) microchannels for interferon-gamma (IFN-gamma) assay. To effectively immobilize the target antibodies, oxygen plasma was first used to activate the inert PMMA. This is followed by poly(ethyleneimine) (PEI) coating, an amine-containing functional polymer. For comparison, protein A was also immobilized through the commonly used amine-glutaraldehyde (GA) chemistry. Oxygen plasma treatment effectively increased the amount of PEI attachment and subsequent binding efficiency of the primary antibody. The antibody immobilized via TR-catalyzed protein A was able to provide much better specific antigen capture efficiency than GA chemistry due to the optimal spacing and orientation. Consequently, by using this new method, the detection signal and the signal-to-noise ratio of the ELISA immunoassay in microdevices were all significantly improved. In comparison to the standard assay carried out in the 96-well microtiter plate, the treated microchannels exhibited a broader detection range and a shorter detection time. And the detection limit was also decreased to 20 pg/mL, much lower than that obtained in other microdevices.


Biotechnol Bioeng


Yuan Y,He H,Lee LJ




Has Abstract


2009-02-15 00:00:00












  • Lewis cell studies to determine reactor design data for two-liquid-phase bacterial and enzymic reactions.

    abstract::Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Woodley JM,Brazier AJ,Lilly MD

    更新日期:1991-01-20 00:00:00

  • Cellulase production from immobilized growing cell composites prepared by radiation polymerization.

    abstract::The cellulase production from immobilized Trichoderma reesei composites prepared by radiation polymerization at low temperature was studied. The production of cellulase from the cells irradiated by radiation was slightly retarded at the initial stage of the culture, but was immediately recovered. The production of cel...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kumakura M,Kaetsu I,Nisizawa K

    更新日期:1984-01-01 00:00:00

  • Cascading effect in bioprocessing-The impact of mild hypothermia on CHO cell behavior and host cell protein composition.

    abstract::A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Goey CH,Tsang JMH,Bell D,Kontoravdi C

    更新日期:2017-12-01 00:00:00

  • Temperature control of growth and productivity in mutant Chinese hamster ovary cells synthesizing a recombinant protein.

    abstract::The use of a temperature switch to control the growth and productivity of temperature-sensitive (ts) mutants was investigated to extend the productive life span of recombinant Chinese hamster ovary (CHO) cells in batch culture. Bromodeoxyuridine was used at 39 degrees C to select mutagenized CHO-K1 cells, which result...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jenkins N,Hovey A

    更新日期:1993-11-05 00:00:00

  • Measurement of heat evolution and correlation with oxygen consumption during microbial growth. Reprinted from Biotechnology and Bioengineering, Vol. XI, Issue 3, Pages 269-281 (1968).

    abstract::A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected...

    journal_title:Biotechnology and bioengineering

    pub_type: 传,古典文章,历史文章,杂志文章


    authors: Cooney CL,Wang DI,Mateles RI

    更新日期:2000-03-20 00:00:00

  • A combined cell-cycle and metabolic model for the growth of hybridoma cells in steady-state continuous culture.

    abstract::The Model presented in this work demonstrates the combination of cell-cycle model with a model describing the growth and conversion kinetics of hybridoma cells in a steady-state continuous culture. The cell-cycle model is based upon a population balance model as described by Cazzador et al. and assumes the existence o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Martens DE,Sipkema EM,de Gooijer CD,Beuvery EC,Tramper J

    更新日期:1995-10-05 00:00:00

  • Synthesis of aspartame precursor: alpha-L-aspartyl-L-phenylalanine methyl ester in ethyl acetate using thermolysin entrapped in polyurethane.

    abstract::Cross-linked polyurethane (PU) was prepared for entrapping thermolysin. Using the immobilized thermolysin (IT), Z-L-aspartic acid (ZA) was reacted with -Lphenylalanine methyl ester (L-PM) in water-saturated ethyl acetate to give only alpha-Z-L-aspartylL-phenylalanine methyl ester (alpha-ZAPM). Ninety-four percent conv...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yang CP,Su CS

    更新日期:1988-08-20 00:00:00

  • Influence of water activity in the synthesis of galactooligosaccharides produced by a hyperthermophilic beta-glycosidase in an organic medium.

    abstract::The present study evaluated the influence of water activity and lactose concentration on the synthesis of galactooligosaccharides (GOS), by means of a hyperthermophilic beta-glycosidase in an organic system. The production of GOS gradually grew as water activity increased in the reaction system; later, their synthesis...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cruz-Guerrero AE,Gómez-Ruiz L,Viniegra-González G,Bárzana E,García-Garibay M

    更新日期:2006-04-20 00:00:00

  • Co-immobilization of dextransucrase and dextranase for the facilitated synthesis of isomalto-oligosaccharides: Preparation, characterization and modeling.

    abstract::Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Erhardt FA,Kügler J,Chakravarthula RR,Jördening HJ

    更新日期:2008-07-01 00:00:00

  • Gas transfer characteristics of a novel membrane bioreactor.

    abstract::We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

    更新日期:1991-12-05 00:00:00

  • Integration of mixing, heat transfer, and biochemical reaction kinetics in anaerobic methane fermentation.

    abstract::An extensive investigation of anaerobic methane fermentation requires identifying the relationship between the physical environment and biological process. In this study, a computational fluid dynamics (CFD) technique was used to characterize bacterial fermentation mechanisms intertwined with mixing and heat transfer ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wu B

    更新日期:2012-11-01 00:00:00

  • Application of cellulases from an alkalothermophilic Thermomonospora sp. in biopolishing of denims.

    abstract::Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Anish R,Rahman MS,Rao M

    更新日期:2007-01-01 00:00:00

  • Production of ethanol by Clostridium thermosaccharolyticum: I. Effect of cell recycle and environmental parameters.

    abstract::The direct microbial conversion (DMC) process for the production of ethanol from lignocellulosic biomass is limited by low volumetric ethanol production rates due to the low cell densities of Clostridium thermosaccharolyticum which is a key organism for ethanol production in this process. Hence, this study focuses on ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mistry FR,Cooney CL

    更新日期:1989-12-05 00:00:00

  • High concentrations of clavulanic acid but not of its degradation products decrease glycerol consumption and oxygen uptake rates in cultures of Streptomyces clavuligerus.

    abstract::Product inhibition of biological production systems is a widely observed phenomenon with prominent implications for the design and ultimately the success of biotechnological processes. In order to investigate whether such effects could limit the maximal concentrations in the production of the beta-lactamase inhibitor ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Brethauer S,Held M,Panke S

    更新日期:2008-06-15 00:00:00

  • Non-invasive self-referencing electrochemical sensors for quantifying real-time biofilm analyte flux.

    abstract::Current techniques for characterizing biofilm physiology lack the signal filtering capability required for quantifying signals associated with real time biologically active transport. Though a great deal was learned from previous investigations, no results have been reported on the characterization of in vivo, real ti...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: McLamore ES,Porterfield DM,Banks MK

    更新日期:2009-02-15 00:00:00

  • Characterization of hybridoma cell responses to elevated pCO(2) and osmolality: intracellular pH, cell size, apoptosis, and metabolism.

    abstract::CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: deZengotita VM,Schmelzer AE,Miller WM

    更新日期:2002-02-15 00:00:00

  • Pulsed addition of limiting-carbon during Aspergillus oryzae fermentation leads to improved productivity of a recombinant enzyme.

    abstract::Fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. The objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bhargava S,Wenger KS,Marten MR

    更新日期:2003-04-05 00:00:00

  • Determination of the cytosolic free NAD/NADH ratio in Saccharomyces cerevisiae under steady-state and highly dynamic conditions.

    abstract::The coenzyme NAD plays a major role in metabolism as a key redox carrier and signaling molecule but current measurement techniques cannot distinguish between different compartment pools, between free and protein-bound forms and/or between NAD(H) and NADP(H). Local free NAD/NADH ratios can be determined from product/su...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Canelas AB,van Gulik WM,Heijnen JJ

    更新日期:2008-07-01 00:00:00

  • 3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs.

    abstract::Three-dimensional cell culture models, such as spheroids, can be used in the process of the development of new anticancer agents because they are able to closely mimic the main features of human solid tumors, namely their structural organization, cellular layered assembling, hypoxia, and nutrient gradients. These prop...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Nunes AS,Barros AS,Costa EC,Moreira AF,Correia IJ

    更新日期:2019-01-01 00:00:00

  • Photo inactivation of virus particles in microfluidic capillary systems.

    abstract::It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ren Y,Crump CM,Mackley MM,Li Puma G,Reis NM

    更新日期:2016-07-01 00:00:00

  • Plasmid maintenance in Escherichia coli recombinant cultures is dramatically, steadily, and specifically influenced by features of the encoded proteins.

    abstract::A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Corchero JL,Villaverde A

    更新日期:1998-06-20 00:00:00

  • Effect of precise control of flux ratio between the glycolytic pathways on mevalonate production in Escherichia coli.

    abstract::Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kamata K,Toya Y,Shimizu H

    更新日期:2019-05-01 00:00:00

  • Analysis of the coupled transport, reaction, and deactivation phenomena in the immobilized glucose oxidase and catalase system.

    abstract::In a previous paper, the overall or macrokinetics of the immobilized glucose oxidase--catalase system has been presented. In this paper a detailed analysis of the interaction of diffusion and reaction in this system will be presented. The mathematical treatment includes two consecutive reactions with two-substrate kin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Reuss M,Buchholz K

    更新日期:1979-11-01 00:00:00

  • Surfactant-Induced hydrogen production in cyanobacteria.

    abstract::Addition of Tween 85 to aqueous suspensions of Anabaena variabilis induced photosynthetic evolution of hydrogen over a time span of several weeks: As much as 148 nmol H(2)/h . mg dry weight was produced in the first week by a suspension containing 4.2 mg dry weight of cells and 77 mM Tween 85. The chemical structure o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Famiglietti M,Hochkoeppler A,Luisi PL

    更新日期:1993-10-01 00:00:00

  • High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture.

    abstract::Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nierode GJ,Gopal S,Kwon P,Clark DS,Schaffer DV,Dordick JS

    更新日期:2019-01-01 00:00:00

  • Mitigation of ammonia inhibition by internal dilution in high-rate anaerobic digestion of food waste leachate and evidences of microbial community response.

    abstract::A high-rate anaerobic digestion of food waste leachate were tested using intermittent continuously stirred tank reactors (iCSTRs) to evaluate how severe ammonia inhibition could be mitigated with internal dilution strategy, and to identify how bacterial and archaeal community respond in genus and species level. Experi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yun YM,Kim DH,Cho SK,Shin HS,Jung KW,Kim HW

    更新日期:2016-09-01 00:00:00

  • Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli.

    abstract::Small interfering RNAs (siRNAs) are invaluable research tools for studying gene functions in mammalian cells. siRNAs are mainly produced by chemical synthesis or by enzymatic digestion of double-stranded RNA (dsRNA) produced in vitro. Recently, bacterial cells, engineered with ectopic plant viral siRNA binding protein...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kaur G,Cheung HC,Xu W,Wong JV,Chan FF,Li Y,McReynolds L,Huang L

    更新日期:2018-09-01 00:00:00

  • Plasmid DNA production for pharmaceutical use: role of specific growth rate and impact on process design.

    abstract::Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bohle K,Ross A

    更新日期:2011-09-01 00:00:00

  • An ultrafiltration membrane bioreactor for the lipolysis of olive oil in reversed micellar media.

    abstract::The enzymatic hydrolysis of olive oil using Chromobacterium viscosum lipase B encapsulated in reversed micelles of dioctyl sodium sulfosuccinate (AOT) in isooctane was investigated in an ultrafiltration ceramic membrane reactor of tubular type, operating in a batch mode. Water concentration was found to be a critical ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Prazers DM,Garcia FA,Cabral JM

    更新日期:1993-04-05 00:00:00

  • Characteristics and role of dynamic membrane layer in anaerobic membrane bioreactors.

    abstract::A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

    更新日期:2016-04-01 00:00:00