Abstract:
:A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especially from Jerusalem artichoke extracts containing large amounts of high polyfructosans ("early" extracts).
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Guiraud JP,Bourgi J,Stervinou M,Claisse M,Galzy Pdoi
10.1002/bit.260290707subject
Has Abstractpub_date
1987-05-01 00:00:00pages
850-8issue
7eissn
0006-3592issn
1097-0290journal_volume
29pub_type
杂志文章abstract::Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACP...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22149
更新日期:2009-03-01 00:00:00
abstract::Potentiometric and amperometric measurements were made with microbial fuel cells containing E. coli or yeast as the anodic reducing agent and glucose as the oxidizable substrate. The catalytic effects of thionine and resorufin on the anode reaction were investigated. Results on the potentiometry, polarization, and cou...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250219
更新日期:1983-02-01 00:00:00
abstract::The dissociation constant, K(d) , is an important parameter for characterizing protein-protein interaction affinities. SUMOylation is one of the important protein post-translational modifications and it involves a multi-step enzymatic cascade reaction, resulting in peptide activation and substrate conjugation. Multipl...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24564
更新日期:2012-11-01 00:00:00
abstract::This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21823
更新日期:2008-08-01 00:00:00
abstract::In this article, we present the factors which influence the stability of the emulsion forming under the circumstances of the direct extraction of penicillin fermentation broth and the conditions of demulsification. A laboratory method has been developed for testing demulsifiers and a quantitative evaluation of the pro...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400208
更新日期:1992-06-20 00:00:00
abstract::Novel amphoteric particles are prepared by an interfacial polyaddition reaction of ethylene glycol diglycidyl ether (EGDGE) and L-lysine in a water-in-oil (W/O) miniemulsion system. The zeta-potential of the copolymer particle as a function of pH indicates a sigmoid curve with an isoelectric point at pH 8.7 due to the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20711
更新日期:2006-02-05 00:00:00
abstract::For the application of immobilized enzymes, fixed bed reactors are used almost exclusively. Fixed bed reactors have specific disadvantages, especially for processes with a deactivating catalyst. Therefore, we have studied a novel reactor type with continuous transport of the immobilized biocatalyst. Flow of biocatalys...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360407
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27113
更新日期:2019-11-01 00:00:00
abstract::This reports on the determination of the overall oxygen transfer coefficient in a mechanically agitated vessel using a randomly pulsed dynamic method. This method consists in exciting the system by randomly switching the inlet gas stream with air or nitrogen with an identical volumetric flow rate. A pseudo-random bina...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370914
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abstract::One of the major bottlenecks in the bioconversion of lignocelluosic feedstocks to liquid ethanol is the recalcitrance of residue following pretreatment, specifically softwood derived residues. Peroxide delignification has previously been shown to effectively aid in the removal of condensed lignaceous moieties from sub...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22768
更新日期:2010-08-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360513
更新日期:1990-08-20 00:00:00
abstract::The cell cycle dependency of foreign gene expression in recombinant mouse L cells was investigated. Two different recombinant mouse L cell lines having the glucocorticoid receptor-encoding gene and the lacZ reporter gene were used in this study. The lacZ gene expression was controlled by the glucocorticoid-inducible m...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1996-05-05 00:00:00
abstract::It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained comp...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260191204
更新日期:1977-12-01 00:00:00
abstract::Protein localization in Saccharomyces cerevisiae was studied with two plasmid systems used as a model: one containing the SUC2 structural gene fused with the MFalpha1 (alpha-factor) promoter and signal-sequence, the other containing the entire SUC2 gene. Special emphasis was placed on the effect of promoter/signal-seq...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340903
更新日期:1989-11-01 00:00:00
abstract::The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260170802
更新日期:1975-08-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260411009
更新日期:1993-04-25 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400218
更新日期:1992-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360204
更新日期:1990-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23058
更新日期:2011-06-01 00:00:00
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21711
更新日期:2008-04-15 00:00:00
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1997-01-20 00:00:00
abstract::The use of microalgae for biofuel production will be beneficial to society if we can produce biofuels at large scales with minimal mechanical energy input in the production process. Understanding micro-algal physiological responses under variable environmental conditions in bioreactors is essential for the optimizatio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23281
更新日期:2012-01-01 00:00:00
abstract::Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10142
更新日期:2002-02-15 00:00:00
abstract::A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs...
journal_title:Biotechnology and bioengineering
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abstract::The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260911
更新日期:1984-09-01 00:00:00
abstract::Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21535
更新日期:2008-01-01 00:00:00