Abstract:
:Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. This model does not recapitulate the complex process of absorption and metabolism of digestive lipids. Here, we introduce a gut-liver chip, which mimics the gut absorption and hepatic metabolism in a microfluidic chip. Absorption of fatty acids through gut layer and subsequent deposition within liver cells was demonstrated. Tumor necrosis factor-α, butyrate, and α-lipoic acid were chosen as model molecules that can affect hepatic steatosis via different mechanisms, and their effects were evaluated. Our results suggest that the gut-liver chip can mimic the absorption and accumulation of fatty acids in the gut and the liver.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Lee SY,Sung JHdoi
10.1002/bit.26793subject
Has Abstractpub_date
2018-11-01 00:00:00pages
2817-2827issue
11eissn
0006-3592issn
1097-0290journal_volume
115pub_type
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更新日期:2007-10-01 00:00:00
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更新日期:1994-02-20 00:00:00
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