Abstract:
:A transient, two-culture model simulating methane production from biomass has been developed. The simulator, based partially on the work by Andrews and McCarty, is capable of calculating the hydrolysis products of several common organic materials, accommodating various substrate feeding modes, and simulating the transient physico-biochemical transport and conversion processes occurring in the biological, liquid, and gaseous phases of a well-mixed reactor. The mathematical representation of this bioconversion system consists of a set of 11 coupled, nonlinear first-order rate equations based on the principles of mass conservation and biochemical reaction kinetics. The model can be used in conjunction with laboratory investigations and as a simulator for evaluating process control strategies and cost developments.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Kleinstreuer C,Poweigha Tdoi
10.1002/bit.260240903subject
Has Abstractpub_date
1982-09-01 00:00:00pages
1941-51issue
9eissn
0006-3592issn
1097-0290journal_volume
24pub_type
杂志文章abstract::Crossflow nanofiltration experiments were performed to investigate the factors influencing the removal of amino acids by a commercially available polymeric thin-film composite membrane. The removals of five monoprotic (Ala, Val, Leu, Gly, and Thr), one diprotic (Asp), and one dibasic (Arg) amino acids in a range of pe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21395
更新日期:2007-10-01 00:00:00
abstract::A dynamic model for the degradation of phenol in a two-phase partitioning bioreactor has been developed based on mechanistic balances around the bioreactor. The key process characteristics including substrate transfer between the organic and aqueous phases, substrate inhibition, oxygen limitation, and cell entrainment...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000120)67:2<224::aid-bit
更新日期:2000-01-20 00:00:00
abstract::Protein switches have a variety of potential applications in biotechnology and medicine that motivate efforts to accelerate their development. Switches can be built by the proper fusion of two proteins with the prerequisite input and output functions. However, the exact fusion geometry for switch creation, which typic...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25852
更新日期:2016-04-01 00:00:00
abstract::In vivo kinetics of Saccharomyces cerevisiae are studied, in a time window of 150 s, by analyzing the response of O(2) and CO(2) in the fermentor off-gas after perturbation of chemostat cultures by metabolite pulses. Here, a new mathematical method is presented for the estimation of the in vivo oxygen uptake rate (OUR...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10480
更新日期:2003-02-20 00:00:00
abstract::We have developed a dual-chambered bioreactor (DCB) that incorporates a membrane to study stratified 3D cell populations for skin tissue engineering. The DCB provides adjacent flow lines within a common chamber; the inclusion of the membrane regulates flow layering or mixing, which can be exploited to produce layers o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27164
更新日期:2019-12-01 00:00:00
abstract::A stimulus-response tracer technique has been used to characterize packed beds of untreated, as well as acid prehydrolyzed, and enzymatically hydrolyzed aspen wood chips. Glucose was used as the tracer. Bulk liquid phase dispersion, interphase mass transfer, and intraparticle diffusion coefficients were determined for...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410310
更新日期:1993-02-05 00:00:00
abstract::A strategy to determine effective diffusion coefficients of proteins in chromatographic gels is presented in this article. An experimental methodology based on frontal liquid chromatography was combined with a numerical methodology based on a mathematical model describing the chromatographic process including the extr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20738
更新日期:2006-03-05 00:00:00
abstract::A two-member methane-utilizing mixed culture of bacteria, formed by combining two pure cultures isolated from a naturally occurring methane-utilizing mixed culture, was studied in continuous culture. From the nutritional requirements and substrate ranges of the pure cultures, a mechanism for the interspecific interact...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260221010
更新日期:1980-10-01 00:00:00
abstract::An analysis of the radial flow cell is presented to show that the assumption of creeping laminar flow should be used cautiously. Simple models which account for the influence of fluid inertial forces over most of the width of the plate are reviewed. A modified Reynolds number is introduced which may be used to test th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270407
更新日期:1985-04-01 00:00:00
abstract::Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27536
更新日期:2020-08-18 00:00:00
abstract::In vitro gliding assay, microtubule translocation by kinesin motor proteins on a surface, has been used as an engineering tool in analyte detection, molecular cargo transport, and other applications. Although controlling the moving direction is often necessary to realize these applications, current direction control m...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26933
更新日期:2019-05-01 00:00:00
abstract::Synthetic mixtures of predominant lignocellulosic hexose sugars were supplemented with separate aliquots of three inhibitory compounds (furfural, hydroxymethylfurfural (HMF), and acetic acid) in a series of concentrations and fermented by the spent sulfite liquor (SSL)-adapted yeast strain Tembec T1 and the natural is...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20838
更新日期:2006-04-20 00:00:00
abstract::Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420303
更新日期:1993-07-01 00:00:00
abstract::A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of cu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10111
更新日期:2002-01-20 00:00:00
abstract::Sugarcane bagasse is an abundant and sustainable resource, generated as a by-product of sugarcane milling. The cellulosic material within bagasse can be broken down into glucose molecules and fermented to produce ethanol, making it a promising feedstock for biofuel production. Mild acid pretreatment hydrolyses the hem...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24984
更新日期:2013-12-01 00:00:00
abstract::The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990720)64:2<240::aid-bit
更新日期:1999-07-20 00:00:00
abstract::High throughput pretreatment (HTPH) and enzymatic hydrolysis systems are now vital for screening large numbers of biomass samples to investigate biomass recalcitrance over various pretreatment and enzymatic hydrolysis conditions. Although hydrothermal pretreatment is currently being employed in most high throughput ap...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24951
更新日期:2013-11-01 00:00:00
abstract::Zymomonas mobilis is a very important gram-negative bacterium having a potential application to simultaneous co-production of biofuel and other high value-added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its ap...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23106
更新日期:2011-07-01 00:00:00
abstract::Coastal bermudagrass was pretreated by a low-temperature ammonia fiber explosion (AFEX) process, which soaked the grass in liquid ammonia and then explosively released the pressure. Saccharifying enzymes were systematically applied to the AFEX-treated grass corresponding to low, medium, and high loadings of cellulase/...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260440914
更新日期:1994-11-05 00:00:00
abstract::The kinetic properties of collagen-bound firefly luciferase have been investigated. Under definite hydrodynamic conditions with low agitation in the reaction medium, the observed behavior is modified compared to the enzyme free in solution: reducing the stirring rate decreases the observed enzymatic activity. But diff...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280804
更新日期:1986-08-01 00:00:00
abstract::Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260480609
更新日期:1995-12-20 00:00:00
abstract::The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360503
更新日期:1990-08-20 00:00:00
abstract::A model for radial flow mammalian cell culture has been developed. Two situations, one with permeable hollow fibers and the other with a porous matrix in the annulus, were considered. The hollow fibers were modeled as continuously distributed sinks. Numerical solutions are presented for the complete model as well as l...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290602
更新日期:1987-04-01 00:00:00
abstract::RNA viruses are capable of undergoing extremely rapid evolution due to their high rates of reproduction, small genome size, and a high frequency of spontaneous mutagenesis. Here we demonstrate that a virus-like, evolutionary state can be created by propagating a phagemid population in a hypermutator strain of Escheric...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000405)68:1<121::aid-bit
更新日期:2000-04-05 00:00:00
abstract::Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (R...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26009
更新日期:2016-11-01 00:00:00
abstract::Neural networks are formed by accurate connectivity of neurons and glial cells in the brain. These networks employ a three-dimensional bio-surface that both assigns precise coordinates to cells during development and facilitates their connectivity and functionality throughout life. Using specific topographic and chemi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20618
更新日期:2005-11-05 00:00:00
abstract::A low-density, porous chitosan/poly-(dl-lactide-co-glycolide) (PLGA) microparticle composite scaffold was produced by thermally induced phase separation followed by lyophilization, to provide a bicontinuous microstructure potentially suitable for tissue engineering and locally controlled drug release. PLGA particles w...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21796
更新日期:2008-07-01 00:00:00
abstract::The anaerobic digestion of a cellulose-enriched fraction of domestic refuse by means of rumen microorganisms in an "artificial rumen" digester was studied. Various combinations of solid and liquid retention times and loading rates were applied to establish optimum conditions for the acidogenic phase digestion of the r...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320604
更新日期:1988-09-05 00:00:00
abstract::A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20728
更新日期:2006-02-05 00:00:00
abstract::Phosphorus-31 nuclear magnetic resonance ((31)P NMR) was used to compare the anaerobic metabolism of glucose by suspended and gel-entrapped Saccharomyces bayanus cells. The fermentation of glucose was carried out in a reaction system with continuous circulation through the NMR sample tube. The intracellular pH and the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410607
更新日期:1993-03-15 00:00:00