CRISPR interference of nucleotide biosynthesis improves production of a single-domain antibody in Escherichia coli.

abstract：:A cybernetic framework is presented which views microbial response in multiple substrate environments as a judicious investment of cellular resources in synthesizing different key proteins according to an optimal regulatory strategy. A mathematical model is developed within the cybernetic framework for the diauxic gro...

journal_title：Biotechnology and bioengineering

authors： Dhurjati P,Ramkrishna D,Flickinger MC,Tsao GT

更新日期：1985-01-01 00:00:00

abstract：:The use of cell numbers rather than mass to quantify the size of the biotic phase in animal cell cultures causes several problems. First, the cell size varies with growth conditions, thus yields expressed in terms of cell numbers cannot be used in the normal mass balance sense. Second, experience from microbial system...

journal_title：Biotechnology and bioengineering

authors： Nielsen LK,Reid S,Greenfield PF

更新日期：1997-11-20 00:00:00

abstract：:The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...

journal_title：Biotechnology and bioengineering

authors： Karlsen LG,Villadsen J

更新日期：1984-12-01 00:00:00

abstract：:Acidic Golgi pH plays an important role in protein glycosylation, one of the critical quality attributes of therapeutic proteins. To determine the intracellular Golgi pH during culture, stable Chinese hamster ovary (CHO) cell clones expressing pHluorin2, a ratiometric pH-sensitive fluorescent protein (FP), in the cis-...

journal_title：Biotechnology and bioengineering

authors： Lee JH,Kim J,Park JH,Heo WD,Lee GM

更新日期：2019-05-01 00:00:00

abstract：:Uranium competitive uptake experiments by Rhizopus arrhizus were carried out at three different solution pH levels and in the presence of different concentrations of competing aluminum ions in order to examine the competing ion effect. The coion effect became more pronounced as the coion concentration in solution and ...

journal_title：Biotechnology and bioengineering

authors： Tsezos M,Georgousis Z,Remoudaki E

更新日期：1997-07-05 00:00:00

abstract：:High methanol concentrations have a negative effect on the growth rate and the biomass yield of growth transients induced by methanol pulses in continuous cultures of Methylomonas L3. The physiological basis of this effect is investigated by measuring the effect of the methanol pulse on the cell energy charge (EC) and...

journal_title：Biotechnology and bioengineering

authors： Chu IM,Papoutsakis ET

更新日期：1987-01-01 00:00:00

abstract：:Equilibrium isotherms for adsorption of bovine serum albumin (BSA) on a new adsorbent, a strongly basic crosslinked chitosan (Chitopearl 2503), which is hard and is not compressed by pressure in a column, have been presented and compared with diethylaminoethyl (DEAE) Sepharose Fast Flow (hard gel). In Chitopearl 2503,...

journal_title：Biotechnology and bioengineering

authors： Yoshida H,Nishihara H,Kataoka T

更新日期：1994-05-01 00:00:00

abstract：:While elastin-like polypeptides and peptides (ELPs) have been used for various stimulus-responsive applications, details of their switching remain unclear. We therefore constructed a novel series of filamentous phage particles displaying a high surface density of short ELPs. The surface display of ELPs did not disrupt...

journal_title：Biotechnology and bioengineering

authors： Hathorne AP,Bermudez H

更新日期：2013-07-01 00:00:00

abstract：:Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...

journal_title：Biotechnology and bioengineering

authors： Chen CI,Taylor RT

更新日期：1995-12-20 00:00:00

abstract：:The development of autoantibodies against factor VIII represents one of the major complications in the treatment of hemophilia A patients. We have employed a novel library system to obtain peptides that specifically neutralize the interaction between factor VIII and these inhibitors. The random peptides are presented ...

journal_title：Biotechnology and bioengineering

authors： Mersich C,Billes W,Pabinger I,Jungbauer A

更新日期：2007-09-01 00:00:00

abstract：:The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption...

journal_title：Biotechnology and bioengineering

authors： Gilbert PA,Kamen A,Bernier A,Garnier A

更新日期：2007-09-01 00:00:00

abstract：:Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a co...

journal_title：Biotechnology and bioengineering

authors： Mehra S,Hu WS

更新日期：2005-09-30 00:00:00

abstract：:A comparative study of antimicrobial activity is done using three different electrospun nanofibers namely-CA, PAN, and PVC used as control and with various amounts of AgNO(3) being treated with UV-irradiation leading to the enhancement of silver nanoparticles. DMF is used as the common solvent which helps to undergo s...

journal_title：Biotechnology and bioengineering

authors： Lala NL,Ramaseshan R,Bojun L,Sundarrajan S,Barhate RS,Ying-Jun L,Ramakrishna S

更新日期：2007-08-15 00:00:00

abstract：:CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...

journal_title：Biotechnology and bioengineering

authors： deZengotita VM,Schmelzer AE,Miller WM

更新日期：2002-02-15 00:00:00

abstract：:The sec-alcohol dehydrogenase activity of whole cells of Rhodococcus ruber DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH...

journal_title：Biotechnology and bioengineering

authors： Stampfer W,Kosjek B,Kroutil W,Faber K

更新日期：2003-03-30 00:00:00

abstract：:Natural oxygen gradients occur in tissues of biological organisms and also in the context of three-dimensional (3D) in vitro cultivation. Oxygen diffusion limitation and metabolic oxygen consumption by embedded cells produce areas of hypoxia in the tissue/matrix. However, reliable systems to detect oxygen gradients an...

journal_title：Biotechnology and bioengineering

authors： Schmitz C,Pepelanova I,Seliktar D,Potekhina E,Belousov VV,Scheper T,Lavrentieva A

更新日期：2020-07-15 00:00:00

abstract：:Protein localization in Saccharomyces cerevisiae was studied with two plasmid systems used as a model: one containing the SUC2 structural gene fused with the MFalpha1 (alpha-factor) promoter and signal-sequence, the other containing the entire SUC2 gene. Special emphasis was placed on the effect of promoter/signal-seq...

journal_title：Biotechnology and bioengineering

authors： Marten MR,Seo JH

更新日期：1989-11-01 00:00:00

abstract：:Recombinant Escherichia coli JM101 was used for the in vivo biocatalytic synthesis of 3-tert-butyl- catechol. The bacterial strain synthesized the laboratory-evolved variant HbpA(T2) of 2-hydroxybiphenyl 3-monooxygenase (HbpA, EC 1.14.13.44) from Pseudomonas azelaica HBP1. The mutant enzyme HbpA(T2) is able to hydroxy...

journal_title：Biotechnology and bioengineering

authors： Meyer A,Held M,Schmid A,Kohler HP,Witholt B

更新日期：2003-03-05 00:00:00

abstract：:This article provides the analysis on biomass yields and energetic yields of the oleaginous yeasts. The biomass yields of the oleaginous yeasts are consistently lower than nonoleaginous microorganisms, whereas their energetic yields are higher. Data inconsistencies of literature are explained by the variation of energ...

journal_title：Biotechnology and bioengineering

authors： Pan JG,Rhee JS

更新日期：1986-01-01 00:00:00

abstract：:Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

journal_title：Biotechnology and bioengineering

authors： Davidson AJ,Ellis MJ,Chaudhuri JB

更新日期：2010-08-15 00:00:00

abstract：:The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...

journal_title：Biotechnology and bioengineering

authors： Speed MA,King J,Wang DI

更新日期：1997-05-20 00:00:00

abstract：:Efficient xylose utilization will facilitate microbial conversion of lignocellulosic sugar mixtures into valuable products. In Escherichia coli, xylose catabolism is controlled by carbon catabolite repression (CCR). However, in E. coli such as the succinate-producing strain KJ122 with disrupted CCR, xylose utilization...

journal_title：Biotechnology and bioengineering

authors： Kurgan G,Sievert C,Flores A,Schneider A,Billings T,Panyon L,Morris C,Taylor E,Kurgan L,Cartwright R,Wang X

更新日期：2019-08-01 00:00:00

abstract：:The simultaneous saccharification and co-fermentation (SSCF) kinetic model described in the companion paper can predict batch and fed batch fermentations well at solids concentrations up to 62.4 g/L cellulose paper sludge but not in batch fermentation at 82.0 g/L cellulose paper sludge. Four hypotheses for the discrep...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Shao X,Lynd LR

更新日期：2009-12-01 00:00:00

abstract：:Zymomonas mobilis is a very important gram-negative bacterium having a potential application to simultaneous co-production of biofuel and other high value-added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its ap...

journal_title：Biotechnology and bioengineering

authors： Dong HW,Bao J,Ryu DD,Zhong JJ

更新日期：2011-07-01 00:00:00

abstract：:Actively growing Escherichia coli C600(pBR322), immobilized within the macroporous matrix of asymmetric-wall hollow-fiber membranes, has been propagated to extremely high densities, typically more than 10(12) cells/mL of accessible void volume, in some regions cells accounting for nearly 100% of the available macrovoi...

journal_title：Biotechnology and bioengineering

authors： Inloes DS,Smith WJ,Taylor DP,Cohen SN,Michaels AS,Robertson CR

更新日期：1983-11-01 00:00:00

abstract：:Regioselectivity of glycosyltransferases offers an important means to overcome the limitations of chemical synthesis of small molecule glycosides. In this study we explore a large multigene family of UDP-glucose:glycosyltransferases of Arabidopsis for their potential as novel biocatalysts for in vitro synthesis and wh...

journal_title：Biotechnology and bioengineering

authors： Lim EK,Ashford DA,Hou B,Jackson RG,Bowles DJ

更新日期：2004-09-05 00:00:00

abstract：:The effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) scaffolds with varying pore geometries (square and hexagonal channels) were investigated. The scaffold with sq...

journal_title：Biotechnology and bioengineering

authors： Aliabouzar M,Lee SJ,Zhou X,Zhang GL,Sarkar K

更新日期：2018-02-01 00:00:00

abstract：:Human skin-derived precursor cells (hSKPs) are multipotent adult stem cells found in the dermis of human skin. Incorporation of hSKPs into split-thickness skin grafts (STSGs), the current gold standard to treat severe burns or tissue resections, has been proposed as a treatment option to enhance skin wound healing and...

journal_title：Biotechnology and bioengineering

authors： Surrao DC,Boon K,Borys B,Sinha S,Kumar R,Biernaskie J,Kallos MS

更新日期：2016-12-01 00:00:00

abstract：:For clinical trials, large amounts of high-titer retroviral supernatants are required. However, retroviral concentration is relatively low compared with other viral vectors. Moreover, less than half of retroviral vectors suspended in a collected supernatant are infectious because of their short half-lives. In this stu...

journal_title：Biotechnology and bioengineering

authors： Kwon YJ,Peng CA

更新日期：2005-05-05 00:00:00

abstract：:Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

journal_title：Biotechnology and bioengineering

authors： Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

更新日期：2012-01-01 00:00:00